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Warner Cell Electrophysiology & Cell Biology Research
Hugo Sachs Elektronic Isolated Organ and Tissue Systems
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Harvard Apparatus
 

New Products in Physiology and Regenerative Medicine!


Products on this page are for Research Use Only. Not for use in humans unless proper investigational device regulations have been followed.







NEW Bi-Ventricular Working Heart System



  • Rat, Guinea Pig, Rabbit
  • Dual Flow up to 500 ml/min
  • Study Right Heart Damage (RVH) and more
  • Simultaneous RV and LV PV Loop
  • Transitions from Langendorff to Working Left Heart to full Biventricular Modes are accomplished by a simple turn of a stopcock
  • Solid State Physiological Perfusion Circuit (S2P2C) ensures repeatable, fully laminar flow
The Hugo Sachs Elektronik- Harvard Apparatus Bi-Ventricular Isolated Working Heart perfusion system utilizes patented flow resistance and compliance chambers to faithfully mimic the in-vivo cardiac preloads and afterloads for both normal and diseased states in mid-sized rodent models (rat, guinea pig, rabbit). The exclusive features of the system create the most physiological isolated heart environment closely resembling the actual resistance of the peripheral vasculature while allowing the entire heart to work as it does in vivo, setting a new standard for Isolated Heart research. This fully-integrated system allows for in-depth ex vivo studies of the effects of Pulmonary Artery Hypertension (PAH), Hypertrophy (RVH), COPD, ARCV, Emphysema and a variety of other diseases characterized by pulmonary vascular dysfunction and right heart pathophysiology.

The system allows measurement of a wide range of cardiovascular parameters with unsurpassed fidelity: Create a Bi-Ventricular Cardiac Electrophysiology Workstation complete with Wilson and Einthoven-Goldberger leads and up to 8 Cardiac Action Potential measurements. Integrate High Fidelity Pressure Volume Catheters for right and left ventricle PV Loop Analysis. Ask us about our custom configurations for your unique application requirements!



NEW Harvard Apparatus – Fluorescence-Based Detection
of pH, CO2 and O2: Rugged, Sterilizable, Sensitive




  • More accurate measurements than traditional methods
  • No electrolytes required
  • Completely Sterilizable sensors
  • USP Class VI-certified sensors are shipped pre-calibrated
  • Minimal chemical degradation means usage life and infrequent recalibration
  • Ideal for Tissue Engineering and Regenerative Medicine applications
 
This implementation of Fluorescence-based Biosensors represent a breakthrough technology in the measurement of typical blood gas parameters in tissue engineering, organ perfusion and general physiology applications.

In contrast to traditional ion-sensitive chemosensors, these optical biosensors use a controlled optical source that emits light signals at specified frequencies which excite an ion-specific sensor spot in contact with the test medium. Changes in the parameter of interest are reported as a proportional change in some characteristic (intensity, phase shift, etc.) of the fluorescence. Because they are inherently self-referencing, this detection technology eliminates the potential for measurement error arising from changes in sample properties (i.e. flow rate, viscosity, etc.) over the duration of an experiment. This ensures stable, drift-free calibration and hence reliable measurements throughout the lifespan of a sensing element. Unlike polarographic oxygen electrodes, this technology is non ion-consumptive. These easy-to-use biosensors are free of the maintenance complications inherent in traditional chemosensing: requiring only cleaning and dry storage, there is no need for electrolyte, membrane changes, or pre-polarization of the electrode amplifier.
 

Click here to download the Polestar Catalog Pages



NEW Micro-Cannulated Vessel Perfusion System
with Advanced Analysis Software




  • Single or Dual Channel
  • 5 ml or 10ml Teflon-coated Chamber Inserts
  • Use on Confocal Imaging Microscope
  • Constant Pressure or Constant Flow Perfusion
  • Add drugs/compounds to perfusate or superfusate
  • Measure intraluminal pressure changes
  • IonOptix Vesacq Vessel Diameter and FloAcq Measurement software
  • Real Time Dual Imaging Analysis
  • Full system packages include:
    • PC and Monitor
    • VESSCAM Imaging camera
    • Dissecting microscope
    • Wireless software-controlled Pumps
    • Chambers & Accessories
 
This system is available in single or dual channel configurations and is used to study physiological function of small microvessels under controlled near-physiological conditions. The mounting cannula are pre-pulled glass and are very easy to align with the three axis alignment system built-in to the chamber. The entire system is ergonomically designed for maximum comfort and ease-of-use with a unique movable platform with separate zones for dissection/mounting and recording not available on any other system.

Constriction or dilation can be measured via pressure signals or by changes in diameter and sample wall thickness via the optional digital edge-detection bundle. The IonOptix VesAcq Software package utilizes line-averaging edge detection which averages contrast over all of the lines in up to 4 regions of interest along the length of the vessel, negating the need for supersaturating the image to drown out the contrast from remnants of blood, fat, folding, etc.

The IonOptix FloAcq module is the first of its kind to perform real-time calculations of important indicators of arterial flow. Researchers can determine whether vasodilation is flow- or pressure-mediated. Additional parameters measured are vascular resistance, shear stress, and Re (Reynolds Number for characterizing laminar or turbulent flow).


NEW Infrared Contrast Imaging (IRCI) for Visualizing
Physiological, Metabolic, and Morphological Features




  • Economical compared to other imaging techniques
  • Easy-to-use: no special rooms & no advanced training - operates just like a digital camera
  • Ideal for visualizing perfusion paths in organs and microfluidics chips
  • Totally non-destructive to samples
  • Add drugs/compounds to perfusate or superfusate
 
Harvard Apparatus, has developed a new way to utilize infrared imaging technology to allow clear visualization of histological, physiological and metabolic phenomena down to the cellular level. Visualize events that are difficult to see in the visible light spectrum such as:
  • Contrast of physical features with no or poor visible contrast
  • Physiological and metabolic changes in live tissue
  • Levels of hydration and vascularization in tissue and organs
  • Application of therapeutic agents through injection or aerosol
  • Hydrodynamics of mixing two liquids: perfusion paths; nutrient flows in cell culture
  • Transparent aerosols
This technology permits quantitative assessment and viewing of biological events that, until now, have relied on subjective observations.

IRCI has been employed as a high-sensitivity, relatively low-cost method in many applications where visible examination reveals little information. This is accomplished by detection of infrared emission which is influenced in a predictable way by surface composition, texture, chemical make-up, thickness, tonicity and temperature. The emissions are interpreted as distinct color patterns on the reported image, clearly identifying both physical and physiological features that cannot be seen with the naked eye or with visible spectrum imaging. Because IRCI utilizes natural ambient IR emission energies radiated by any object, it is particularly well suited to sensitive physiological and regenerative medicine preparations where cells and tissue may be damaged by exposure to contrast agents or certain light frequencies.
 

Click here to download the IRCI Catalog Pages.

Click here to download the IRCI and Stem Cell Injection Poster.



NEW In-Incubator Cell Culture Stimulation



  • Ideal for Cardiac Tissue Engineering
  • Allows treatment of cells in their native environment
  • Prevents de-differentiation of adult cardiomyocytes
  • Increase transfection efficiency when using viral vectors
  • Configuration for up to 8 Channels
  • 4-well, 6-well, and 8-well plate electrode options
  • Mimic Tachycardia
  • For stimulation fo cardiac tissue, skeletal muscle,
    stem cells, cell-lines, cardiogenic fibroblasts, neuronal fibroblasts, osteoblasts
Cells need exercise, too! Use this multiplexing in-incubator cell culture stimulator to keep your cells in shape by preventing de-differentiation or by encouraging pre-commitment. The C-Pace EP is a multi-channel stimulator designed for the chronic stimulation of bulk quantities of cells in culture incubators. This pacer emits bipolar stimuli, greatly reducing electrolysis at the electrodes.

In addition to standard pacing protocols, the EP has flexible pacing protocols for arrhythmia, exercise, and irregular pacing. Advanced TTL triggering allows switching to the next programmed pulse train. Functionality can be generalized (e.g. acute pacing) using the Gate and Aux outputs.

The primary benefit of this system is that it allows the researcher to treat the cells in a manner consistent with their native environment. Like using proper maintenance media and incubation, chronic culture pacing will help induce and/or maintain phenotype over time, a key necessity for utilization in cardiac tissue engineering.
 

Click here to download the CPace-EP Catalog Pages



Harvard Apparatus Regenerative Medicine Products
 

Advancing Regenerative & Tissue Engineering
with NEW Systems for...

 
  NEW 3D Bioreactors Systems
 

Harvard Apparatus/Hugo Sachs Elektronik has developed new systems to assist the regenerative medicine and tissue engineering researchers in advancing and accelerating 3D bioreactor experimentation. The ORGANizer™ Series solves the problems of simulating the proper physiological environment to accomplish successful tissue engineering or regenerative organ experiments. Using patented and proprietary technologies Harvard Apparatus has created a biomimic environment for specific organ or tissue generation. This provides you better research faster and at less overall cost.

The Harvard Apparatus ORGANizer™ Series has been designed in conjunction with leading regenerative researchers around the world and has been employed in successful regeneration experiments. The ORGANizer™ Series also incorporates the 109 years of experience Harvard Apparatus has in developing and manufacturing specialty physiological products and the expertise of Harvard Apparatus’ world leading Hugo Sachs Elektronik group in developing isolated organ and perfusion systems. Hugo Sachs Elektronik systems for heart and lung have been used for regenerative organ research.

     
  NEW Cell Engineering Products
  Cell & Drug Delivery by Hand
or Stereotaxic Mounting
 

The PHD ULTRA™ Nanomite has a foot pedal for activation of injections. It has methods that can be called up with the touch of the LCD touch screen. With high accuracy and precision this cell delivery system easily delivers low nanoliter to tens ml/min injections with unmatched performance. This is combined with our micro needles to gauge 37. The Nanomite is light weight and easy to hand Hold.

The PHD ULTRA™ design for fast bolus infusion of 1ml/sec, able to deliver low nanoliter injections as well. Flow ramping for superior volume injections into organs with minimal physiological impact.

Harvard Apparatus also has a electroporation, electrofusion, femtomole cell injectors and liposome prep systems to advance your cell and tissue engineering experimentation
     
  A Complete Family Of Cell Harvesting Products From Animal Models
 
  • Surgical Equipment
  • Surgical Instruments
  • Operating Tables & Lights
  • Ventilators from Mice to Large Animals
  • Anesthesia Circuits
  • Infusion Pumps
  • Vital Sign Monitors
  • ...and More!
     
  Physiological Assessment Analysis Tools–
How Good Is Your Engineered Product
 

Ussing Chambers, Bilayer Workstations, Patch Clamps, Tissue Baths and Organ Baths can help you assess the physiology and electrophysiology of you engineered product.

 

NEW 3D Bioreactor Systems
for Regenerative Medicine

Bioreactor for Hollow Organs, Bronchus,
Trachea & Blood Vessels
 
  ORGANizer™ Series Model 100 “In Breath”
 
 
 
  • Facilitates cell seeding procedures on both sides of a 3D tubular matrix, ensuring homogeneous plating
  • Allow seeding and culturing of different cell types on either side of the tubular scaffold
  • Enhance oxygenation of the culture medium and mass transport (oxygen, nutrients and catabolites) between the medium and the adhering cells
  • Stimulates the cells with hydrodynamic stimuli, favoring the metabolic activity and the differentiation process
  • Allows the achievement and maintenance of sterility and other criteria of Good Laboratory Practice (GLP), simplicity and convenience
  • Permits the possibility of automation and scale-up/-out
 

For the Development of Tissue-Engineered Hollow Organs

In Breath is a rotating double-chamber bioreactor designed for cell seeding and culturing on both surfaces of a tubular matrix and includes rotatory movement of the scaffold around its longitudinal axis. A polymeric culture chamber houses the biologic sample and the medium for the whole culture period. Cylindrical scaffold holders are constructed with working ends of different diameter - to house matrices of diverse dimensions - and a central portion of smaller diameter to expose the luminal surface of the matrix for cell seeding and culturing. A co-axial conduit links the inner chamber to the external environment through an appropriate interface at the chamber wall which provides access to seed and feed the luminal surface of the construct. Secondary elements moving with the scaffold holder induce continuous mixing of the culture medium to increase oxygenation and mass transport. The cell/matrix construct is moved by a DC motor (0-5 rpm adjustable) separated from the culture compartments. The connection between the motion unit and the culture chamber allows the first to remain in the incubator for the whole culture period, moving the chamber independently every time is needed (i.e. sampling, medium exchange). An external control unit regulates and monitors rotation. Autoclavability, ease of handling under sterile conditions, reliability and precision ensure the full compatibility of the device with the GLP rules (Good Laboratory Practice).

So Advanced It’s Simple

Reactor container and spindle are made of Polysulphone, Teflon and 316 Stainless Steel allowing for:

  • Sterilization
  • Chemical inertness
  • Biological compatible material
  • Transparent for excellent visualization

Other Features

  • Reactor has quick fit spindle allowing the easy removal of the spindle and associated tubular organ for analysis or to take apart for sterilization
  • Rotational spindle assures proper oxygenation
  • Spindle has inner flow channels to assure inner tissues oxygenation

Easy-To-Use-Controller

  • Easily removable scaffold holder
  • Chamber fits in incubation chamber with controller outside
  • Offset cover allows for oxygenation of sample by non sheering ambient air contact
  • Quick release and disassemble of parts makes sterilization easy
   
 
Please Call for Ordering Information and Pricing
   
  Whole Heart Decellularization/Recellularization
Chamber for Regenerative Medicine
  Langendorff & Working Heart Perfusion System
 
 
 
  • Low flow resistance and dead space volume, minimize perfusion artefacts
  • Compact, does not require high water columns, suitable for hearts from hypertensive animals (perfusion pressure up to 300 mmHg is possible)
  • Constant pressure or constant flow in one unit
  • Drug injection pathway built directly into aortic perfusate stream
  • Modular system grows with your application
  • System specific electrodes and holders for precise measurements
  • All electrodes, catheters etc. are placed around the heart to have easy and direct access

APPLICATIONS

  • Cardioplegia studies
  • Heart transplantation models
  • Electrophysiology studies Monophasic Action Potential (MAP) single and multiple ECG measurements, dispersion of ventricular repolarization
  • In-depth hemodynamic applications including study of cardiac flow, LVP and pressure-volume relationships

WORKING HEART BENEFITS & FEATURES

  • Optimized atrium cannulating conditions
  • Easy to switch from the Langendorff mode (LH) to the working heart mode (WH) and back.
  • Temperature and oxygen loss minimal
  • Short atrial fill time (low flow resistance) resulting in optimal ventricle filling
  • Built in port for insertion of a catheter transducer for left ventricular pressure or pressure-volume measurement
  • Physiologic atrial and arterial pressure and flow waveforms
  • Compact aortic block
    • Exclusive patented Membrane Afterload System and compliance chamber, mimic physiological cardiac afterload and avoid damage to heart valves that occurs with water column based system
    • Integrated flow probe option for accurate real-time aortic flow measurement
  • Real constant preload system
  • Physiologic atrial pressure waveform
  • No stress on mitral valve by the pump like in constant flow mode
  • Preload pressure independent on the atrial flow
  • Integrated flow probe option for accurate real-time atrial inflow measurement (cardiac output)
 

The IH-9 utilizes the proven architecture of our smaller IH-5 to set the standard for isolated heart perfusion in minipigs and small domestic pigs. Engineered for the increased flow produced by these species, the IH-9 offers ultimate perfusion stability and real physiological conditions for longer, more relevant recordings with fewer artifacts.

Both Langendorff and Working Heart versions allow a choice of measurement capabilities with dedicated capability packages available for specialized applications.

Perfusion of such larger hearts creates the opportunity for detailed study of cardioplegia and reperfusion after cardioplegia. With dedicated transducers, electrodes, positioners, amplifiers and software for a complete cardiac electro?physiology workstation, the IH-9 allows cardiovascular studies as well as measurement and analysis of multiple ECG and MAP signals.

Additional Options Include

  • Transducers, probes and amplifiers for: realtime flow, isovolumetric LVP,
    temperature etc... (see section I)
  • Dedicated holder for measurement of MAP-potentials and ECG (see page K14)
  • Dedicated optional perfusion circuit for additional perfusion with eg. perfluorcarbon
    emulsion and with port for Cardioplegia solution application
  • Dedicated software ISOHEART for data acquisition and analysis (see pages K79 to K80)
  • Software option to ISOHEART for flow proportional drug addition using a syringe pump
  • Electrodes and stimulator for pacing (see page K70 and section I)
  • Sensor and amplifier for oxygen consumption measurement
  • Large choice of accessories: thermocirculator, jacketed reservoir, holders etc...
    (see pages K65 to K75

System Description

The functional principle of the IH-9 is the same as for the IH-SR and IH-5 The IH-9 is improved for low volume and high environment stability.

All of the components are mounted on the main heart cover chamber, except for the pumps, the oxygenator, the thermostatic circulator and the PLUGSYS amplifier system. Special attention has been made to ensure that all critical connecting tubing are maintained short, thus avoiding any cooling through a temperature gradient to ambient temperature and any system given flow resistances.

The key part of the system is the unique aortic block which is the interface between the heart and the IH-9. The bubble trap, compliance chamber, patented flow resistance membrane, infusion and measurement ports are all integrated into this single specialized component.

The modular nature of the IH-9 allows the system to evolve along with your research. A Langendorff system can easily be upgraded to full Working Heart; and any IH-9 can be upgraded with special purpose capabilites like multi-channel ECG and MAP for a complete cardiac electrophysiology workstation.

The connection to the heart is made through interchangeable aortic cannulae and atrial cannulae in working heart extension. The heart chamber acts as reservoir and the perfusate containing erythrocytes is oxygenated by a special membrane oxygenator. In the Langendorff mode, heart contractility is measured using a balloon inserted into the left ventricle and connected to a pressure transducer.

Additional equipment required: thermocirculator, peristaltic pumps, transducers, monitoring system setup using the PLUGSYS Amplifier System. Data acquisition and Evaluation using ISOHEART software. See pages K79 to K80.

Cardioplegia Option

A special option allows heart transplantation and cardioplegia studies. With this option the heart can be perfused from a second perfusion circuit in retrograde mode using a different perfusion solution e.g. perfluocarbon emulsion. Cardioplegia solutions are injected or infused via a dedicated port.

 
   
 
Order # Product
PY2 73-2360 Basic Unit for Isolated Heart Size 9 Type 842
Cardioplegia Option
PY2 73-2362 Additional Perfusion Circuit to IH-9
PY2 73-2363 Cover for Heart Chamber for Cardioplegia Cooling
PY2 73-2262 Cardioplegia Cooling Chamber
PY2 73-2481 Low-Temperature Thermostatic Circulator RE 207
Please Call for Pricing
   
 

Whole Lung Decellularization/Recellularization
Chamber for Regenerative Medicine

 
 
 
  • Optimized temperature conditions for the isolated lung, unique jacketed thoracic chamber
  • Optional operating table for non-damaging in-situ preparation
  • Negative-pressure ventilation similar to in-vivo condition or positive pressure ventilation available
  • Low flow resistance and dead space volume, minimize perfusion artifacts
  • Unique built-in pneumotachometer and air humidifier with small dead volume
  • Drug injection pathway built directly into pulmonary perfusate stream
  • Unique compensation system for vascular transmural pressure changes
  • Dedicated option for continuous measurement of lung weight (Edema)
  • More measurement parameters than any other system

APPLICATIONS

  • Studies on combined respiratory and vascular effects
  • Physiology:
    • Hypoxic vasoconstriction
    • Edema formation
  • Pharmacology:
    • Action of histamine, arachinodic acid metabolites
  • Inhalation toxicology:
    • Environmental toxins (ozone, sulphur dioxine)
  • Biochemistery:
    • Metabolic activity (biogenic amines, prostacyclin, angiotensin..)
    • Surfactant biochemistry

ADDITIONAL OPTIONS
INCLUDE

  • Dedicated PLUGSYS ventilator module for negative or positive pressure ventilation VCM (see page I58)
  • Dedicated PLUGSYS module for regular induction of hyperinflation of the lung TCM (sigh, augmented breath) (see page I58)
  • Transducers and amplifiers for respiratory mechanics measurement: airflow, tracheal pressure, thoracic chamber pressure
  • Transducers and amplifiers for perfusion measurement: realtime flow, perfusion pressure, venous pressure, temperature etc... (see section I)
  • Dedicated software PULMODYN?for data acquisition and analysis of respiratory mechanics and perfusion (see pages I90 to I91)
  • Optional connection for external ventilator (see section F) for testing barotraumatic ventilation
  • Optional connection for supplying alternative gas mixtures and/or aerosols
  • Sensor and amplifier for continuous perfusate analysis (pO2, pCO2, pH)
  • Deoxygenation using membrane oxygenator of blood or erythrocyte containing perfusion
  • Large choice of accessories: thermocirculator, jacketed reservoir, holders etc... (see pages K65 to K75)
 

 
 

Basic System

The basic setup consists of a Plexiglass base with two stable vertical steel columns. The main system is mounted on a Plexiglass panel which is fixed on the two steel columns. This allows the system to be modified or extended to suit different versions or for special applications.

The essential part of the system is the artificial thorax. It consists of a water-jacketed glass chamber sealed by a special cover. The cover is mounted on the Plexiglass panel, the glass chamber is fixed underneath it with two metal clips. The inner part of the cover is the organ holder with the connections for cannulating the trachea (ventilation), the pulmonary artery and the left atrium (perfusion).

Ventilation of the lung is possible under negative pressure as well as under positive pressure (the latter is essential during the preparation phase). The ventilation parameters are controlled with a special PLUGSYS Ventilator, the "Ventilation Control Module" (VCM, Type 681).The ventilation medium (air or gas mixture) is constantly humidified by the built-in humidifier.

An optional operating table with trough is mounted on the base plate in front of the artificial thoracic chamber. The organ holder with the connections for the trachea (ventilation) and for the pulmonary artery and pulmonary vein (perfusion) can be removed from the main cover to allow an easier preparation and optimal non traumatic lung transfer from the animal to the system. The lung is continuously ventilated at positive pressure until it is placed into the artificial thorax. Then a simple valve permits instantaneous changeover to negative pressure ventilation.

Perfusion

Lungs can be perfused by either constant flow or constant pressure. Although constant flow perfusion may mimic the in vivo situation more closely, it has the disadvantage that hydrostatic edema becomes inevitable during vasoconstriction. Constant pressure perfusion permits higher perfusate flow rates since vasoconstriction decreases perfusate flow and hydrostatic edema is less likely to occur.
Can be configured in one of three different perfusion modes:

  1. Constant Flow Perfusion
  2. Constant Pressure Perfusion
  3. Dual System to Switch from Constant Flow to Constant Pressure and Vice Versa

Lung Weight Changes

Continuous measurement of weight changes of the lung is required for monitoring edema formation. This is possible during negative pressure ventilation inside the thoracic chamber by using the HSE-Edema balance LS 30 (weight transducer) (PY2 73-0593). Weight changes induced by raising or lowering the perfusion pressure can be used to assess vascular permeability by determining the filtration coefficient.

Extensions

Several adapters are available to work with ventilators, to supply different air mixtures to the lungs or to connect aerosol nebulizer for aerosol challenge.

 
 
Order # Product
PY2 73-2266 Basic Unit for Isolated Perfused Rat
and Guinea pig Lung Size 2 Type 829 (IL-2)
PY2 73-1741 VCM-P PLUGSYS Ventilation Control Module
Please Call for Pricing
 

 
 

The PHD ULTRA™ Nanomite for Cell & Drug
Delivery When You Have Limited Samples
for Cell Therapy

 
 
 
  • Light weight makes ideal hand-held or stereotaxic injection
  • Easy-to-use LCD color touch screen with GUI interface
  • Multiple methods make running simple or complex methods easy
  • High performance in a small package
  • 1000 times better than manual syringes
 

Foot pedal activation of injections for hands off injections. Methods that can be called up with the touch of a button and completely re-load a new methods parameters.

With high accuracy and precision this cell delivery system easily delivers 1 to 100µl injections with unmatched performance from up to a 1ml syringe.

This is combined with our micro needles down to gauge 37. Light weight and easy to mount or hand Hold.

 
 
Order # Product
PY2 70-3601 PHD Ultra™ Nanomite
Please Call for Pricing
 
  The PHD ULTRA™ for Cell & Drug Delivery
for Small to Large Fast Bolus Injections
for Cell Therapy
 
 
 
  • Inject fast bolus of large or small volumes in a second
  • The highest accuracy and precision available
  • Programmable with multiple methods
  • Can be controlled by foot pedal or computer
  • Easy-to-use icon interface
  • CE, ETL, CSA, UL and CB scheme Global compliance
  • Delivery from picoliter to hundreds of Ml/min
  PHD ULTRA™ can deliver compositional variations automatically, % mixing of 2 or 3 liquids Concentration mode can automatically adjust the flow to dose animals of different weights with the same % dose. Hundreds of methods can be stores for one button recall, making PHD ULTRA™ the ultimate in easy to use. Methods can be e-mailed and download from your PC into the pump. Share a method with a collegue or duplicate a complex experimental set-up with an e-mailed method. PHD ULTRA™ Series is ideal for electrospinning and dosing reactors (micro and macro).
 
 
Order # Product
PY2 70-3005 Infuse Only PHD ULTRA™
PY2 70-3006 Infuse/Withdraw PHD ULTRA™
PY2 70-3007 Programable Infuse/Withdraw PHD ULTRA™
Please Call for Pricing
 
  Advanced Cell Engineering
& Regenerative Medicine Solutions
  SWS-10, SWS-60 & SWS-140 Syringe Warmers
 
 
Order # Model Product
PY2 64-1584 SWS-10 Syringe Heater for 10 cc Syringes
PY2 64-1560 SWS-60 Syringe Heater for 60 cc Syringes
PY2 64-1585 SWS-140 Syringe Heater for 140 cc Syringes
PY2 64-1545 TC-124A Temperature Controller, 240 VAC US
PY2 64-1545E TC-124AE Temperature Controller, 240 VAC Europe
PY2 64-1655 TC-144 Temperature Controller
PY2 64-1606 BAC-1 Battery Adapter Cable
Please Call for Pricing
 
  SC-20 Dual In-line Solution Heater/Cooler
 
 
 
  • Heats and cools from 0° to 50°C
  • Compatible with Warner Series 20 Chambers
  • Optimized for use with the CL-100 Bipolar Temperature Controller
 

In-line solution heating has proven to be one of the most effective methods of maintaining the temperature of perfusion solutions. The SC-20 Dual In-line Solution Heater/Cooler utilizes Peltier thermoelectric devices to regulate temperature both above and below ambient levels.

The SC-20 is designed to thermally regulate one or two solutions at the same temperature. Solution temperature can be maintained at 0°C at flow rates of 2 ml/min., 5°C at 5 ml/min., or as high as 50°C at 5 ml/min.

An integral water jacket is used to remove excess heat from the SC-20 peltier device. Running water either from a tap or a large reservoir can be used. Flow rates as low as 4 liters per hour are sufficient to maintain cooling efficiency.

The SC-20 can be used with either one or two discrete perfusate solutions, or with a solution/gas combination. When coupled with a PHC Series Imaging Chamber Heater/Cooler Jacket, the SC-20 provides an effective means of temperature control in a Warner chamber, even in the absence of solution flow.

Each SC-20 is supplied with a TA-29 Thermistor Cable Assembly for monitoring the bath temperature during use, 10 feet of PE-160 tubing and 10 feet of 1/8" I.D. x 1/4" O.D. Tygon tubing.

 
 
Order # Model Product
PY2 64-0353 SC-20 Solution Heater/Cooler Two Line
PY2 64-0352 CL-100 Bipolar Temperature Controller
Replacement Parts
PY2 64-1545 TC-124A Temperature Controller, 240 VAC US
PY2 64-1545E TC-124AE Temperature Controller, 240 VAC Europe
PY2 64-1655 TC-144 Temperature Controller
PY2 64-1606 BAC-1 Battery Adapter Cable
Please Call for Pricing
 
  Valves/Controllers (Fluids & Pressure)
& Fluidic Chips
 
 
  Cell Injection System
 
 

Microinjection is a well established technique used routinely for injection of animal cells, tissues and embryos to insert genetic material such as DNA, RNA, proteins and macromolecules directly into animal cells or embryos a cell cytoplasm or nucleus. Genetic material is inserted via a needle varying in size from 0.5uM to 20uM in diameter. The needle penetrates the cell membrane and/or the nuclear envelope. The process is performed, observed and controlled by using a micromanipulator specialized microscope set up. This technique is widely used by researchers for genetic engineering of cells by modifying, silencing or creating knockouts to study gene targeting and function. Microinjection and electroporation can be combined for the transfection of mammalian cells and tissues for gene therapy applications. This highly effective method is routinely used to insert genes into early stage pronuclear embryos for transgenic development applications for therapeutics. Microinjection is routinely used for nuclear transfer applications, cell biology and viral studies.

 
  Manifold/Connectors, Needles & Tubing
 
 

Tubing, Connectors and Manifolds

Harvard Apparatus has a wide range of tubing, connectors and manifolds so you can create the fluid pathway to meet your specific requirements. We offer many types of tubing of different materials and sizes. Our fluid manifolds start at 2 channels and go all the way up to 24 channels. We have a multitude of connectors that allow you to complete the fluid connections and complete your system. Please call our technical support staff for additional information.

 
  Sensors (Direct & Side Stream)
 
 
  • Air flow
  • Bio-Electric signals
  • Electrodes
  • Flow
  • Force
  • Hygrometers
  • Ion Sensors
  • Pressure
  • Respiration
  • Spill
  • Sound
  • Time
  • Temperature
  • Special Nibp, Heart Rate, O2,
    Co2, Ekg, Map
 
  Battery Powered Spill Sensor System
 
 
 
  • Protect your microscope
  • Audible alarm
  • TTL cutoff circuitry
  • Compatible with upright and inverted microscopes
  • Easy set up and installation
  • Complete system
 

Warner Instruments is pleased to provide a system to detect solution leaks before they cause a problem. This system is ideal for all environments where leak detection is critical such as on a microscope or in a chemical cabinet.

The heart of the system is comprised of a special moisture sensitive mat that has been specifically developed for detecting the presence of fluids. As little 3 drops of liquid are sufficient to trigger the system.

A simple connection between the moisture sensitive mats and the spill sensor controller arms the system. When as little as three drops of liquid come in contact with the moisture sensitive mat an audible alarm is sounded. In addition, an alarm condition switches the state of a regulated TTL output on the Spill Sensor allowing you to dynamically control attached devices.

The Spill Sensor System is supplied with four 30 x 30 cm mats that can be cut to any size or shape using sharp scissors or a straight edge.

 
 
Order # Model Product
PY2 64-1546   Spill Sensor System, Battery Powered
Accessories and Replacement Parts
PY2 64-1522 MAT-1 Overflow Sensor Replacement Mats, set of 4
PY2 64-1523 MAT-2 Overflow Sensor Mats and Cable (4 Mats)
PY2 64-1589 JC-1 Jumper Cable Used to Connect Two Overflow Sensor Mats
Please Call for Pricing

Breakthrough Products for Regenerative Medicine
2011 Regenerative Medicine Brochure
100's of Innovative products developed with leading scientists & surgeons!
  • Injection & Perfusion
  • Bioreactors
  • Biosensing
  • Total Solutions
  • Total Support


Announcing the new
PHD ULTRA™ Syringe Pumps

Click here for more information about PHD ULTRA™ Syringe Pumps and to download a brochure.

  • Patent pending mechanical design provides highest accuracy/precision & smoothest flow
  • Easy operation with advanced touch screen
  • Advanced EZ PRO™ software for PC functions without a PC.


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