Panlab Harvard Apparatus NIPB system provides an easy and reliable technique to measure systemic blood pressure and cardiovascular parameters in rodents without any invasive catheterization.

The pulse signal level (heart rate) is permanently monitored on the LCD display while an internal pressure transducer is constantly measuring the current pressure applied on the cuff. Measurement process involves several steps which are performed automatically; systolic and diastolic blood pressure and calculated mean blood pressure are visualized in real time on the digital display of the control unit. With regards to the systolic pressure, it can be achieved either once the heart pulse disappears or when the first pulse reappears (user selectable) after the occlusion process.

Single and Multiple Animals Systems are available. The Multiple Animal Systems allows a sequential measurement of 6 to 12 animals without having to change the pulse transducers and tails cuffs. A built-in air pump ensures automated cuff inflation/deflation at a constant and even rate. In order to avoid deterioration of the animal?s tail after submitting animals to unnecessary high occlusion pressures, the automated deflation is carried out for each animal and separately, once their own systolic value is reached. Real values (without linear regression calculation) are thus achieved while remaining easily traceable: visualization of the signal through data acquisition software NIBPChart. All units can be used for rats, mice or dogs, using specific pulse transducers and cuffs. All accessories are supplied separately. Once you've chosen the control unit, you will need to choose the appropriate transducer/cuff and animal holder (restrainer). These accessories need to be ordered separately. Restrainers are not required for anesthetized animals. An electronic simulator, heater and scanner are also available if needed.

The requirement of a warm environment for the animals is critical for accurate blood pressure measurements. To provide adequate conditions for NIBP measurement, the system can be used with a warming chamber which provides a comfortable and heated environment necessary to produce peripheral vasodilatation and isolate the animal from external noise.

Parameters Measured
• Systolic blood pressure
• Diastolic blood pressure
• Mean blood pressure
• Heart rate

Components included
• Control Unit with RS-232 serial port (except LE 5001)
• SeDaCom software (except LE 5001)
• Instructions manual
• Cables, connectors
• Set of two spare fuses
• 2 year warranty

Options
• PC interface and USB connection for monitoring of pressure and pulse wave signals. Chart™ software included simple or multiple animals heating units

Citations
Ambuhl PA et al. (2007) A vaccine for hypertension based on virus-like particles: preclinical efficacy and phase I safety and immunogenicity. J. Hypertencion. 25(1): 63-72. (mouse, rat, Switzerland)
Amadeu TP et al. (2007) S-nitrosoglutathione-containing hydrogel accelerates rat cutaneous wound repair. J. Eur. Aca. Dermatol. Venereol. 21(5): 629. (LE5001, rat, Brazil)
Moreira-Rodrigues M et al. (2007) Renal Dopaminergic System Activity in Uninephrectomized Rats up to 26 Weeks after Surgery. Am. J. Nephrol. 27(3): 232-239. (rat, Portugal)
Potenza MA et al. (2007) Epigallocatechin Gallate, a Green Tea polyphenol, improves endothelial function and insulin sensitivity, reduces blood pressure, and protects against myocardial ischemia/reperfusion injury in spontaneously hypertensive rats (SHR). Am. J. Physiol. Endocrinol Metab. In process. (LE5001, rat, Italy, USA)
Trevenzoli IH et al. (2007) Neonatal hyperleptinaemia programmes adrenal medullary function in adult rats: effects on cardiovascular parameters. J. Physiol. 580(2): 629. (rat, Brazil)