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Home Electrophysiology Products Oocyte Clamp Equipment OC-725C Oocyte Clamp Amplifier


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OC-725C Oocyte Clamp Amplifier

The OC-725C Oocyte Clamp is designed for two-electrode, whole-cell voltage clamping of Xenopus oocytes. The dedicated design of the OC-725C includes such features as high compliance voltage (±180 V) and unique bath clamp circuitry. These same features also make the OC-725C ideal for clamping other large cells and cell structures such as squid axons. Improvements to this version include an extended current measuring range, decreased noise level and a 4-pole Bessel filter.
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Item# Description
640028 OC-725C Oocyte Clamp Supplied with 7250V voltage headstage, 7251I bath clamp headstage, 7259C current cable, model membrane and rack mount hardware. Specify line operating voltage if other than 100-130 VAC. One straight holder and one 45° holder nee

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Full Description Back to Top


The OC-725C Oocyte Clamp is designed for two-electrode, whole-cell voltage clamping of Xenopus oocytes. The dedicated design of the OC-725C includes such features as high compliance voltage (±180 V) and unique bath clamp circuitry. These same features also make the OC-725C ideal for clamping other large cells and cell structures such as squid axons. Improvements to this version include an extended current measuring range, decreased noise level and a 4-pole Bessel filter.

Fast Stable Voltage Clamping
The OC-725C combines high AC and DC gains and a voltage compliance of ±180 volts to insure fast, nonsaturating clamp performance under nearly any condition. The AC clamp gain is variable up to 2000. An additional DC gain of 1 x 106 may be employed for high conductance cells (leaky oocytes).
Two clamp speeds are available: The Slow mode is used for screening oocytes or for applications not requiring fast response times. The Fast mode is used for accurate voltage clamp of fast whole cell currents. Clamp response time in the Fast mode is 350 µsec (10-90% rise time) when applying a 100 mV step to a model cell, see specifications on facing page.

Improved Bath Clamp Headstage
The current measuring range of the OC-725C bath clamp headstage has been extended at both ends by the addition of a 3 position range multiplier. Smaller currents are amplified to usable levels and larger currents up to 1 mA can be recorded without output saturation. The unique design of the bath clamp eliminates the need for series resistance compensation. It provides an accurate measurement of bath current by creating a virtual ground in the bath while simultaneously clamping the bath potential at zero.

Voltage Headstage Probe
The voltage measuring headstage is a single-ended, high-impedance probe. Its small size, convenient mounting rod and 2 meter cable make for easy attachment to a micropositioner. The headstage input is a 2 mm diameter pin. An electrode holder with a 2 mm jack (supplied) mounts directly on the headstage.

Voltage & Current Meters
Independent meters provide simultaneous displays of membrane voltage Vm and membrane current Im. To assure proper impalement of the current electrode, the current meter displays membrane potential Ve from the current electrode before the clamp circuit is turned on.

Clamp Commands
The internal Hold control is a digital push button control with two ranges; ±1 to 99 mV (x1) and ±2 to 198 mV (x2). Hold can be incremented in steps of 1, 2, 10 and 20 mV for I-V studies. External command signals applied to Command IN ÷10 are attenuated to reduce noise from the command source. Hold and external commands are summed.

Additional Features
• Buzz controls (1 kHz square wave) for each electrode aid in penetration of cell membranes with a minimum of leakage.
• Overload alarm (audible and visual) indicate when the compliance voltage is exceeded safeguarding the oocyte and indicating that current records are subject to saturation.
• DC Offsets for both voltage and current electrodes.
• Electrode Test for both electrodes.
• Capacity Compensation for the Vm voltage input.

Electrode Holders
Two vented electrode holders with silver wires are typically required with the clamp; a straight type for use with the voltage headstage and a 45° type with mounting handle for use with current electrodes. Vents have been added to the electrodes to prevent pressure build-up inside the electrode which can damage oocytes.
These holders are available separately. Select the holder based on your glass outer diameter. Unvented holders are available and have a “N” at the end of the model number. Vented holders have a “V” on the end of the model number. A two meter length cable assembly is provided to connect the current electrode holder to the clamp.

Dual Oocyte Studies
Some studies, such as voltage control across gap junctions, require the clamping of two oocytes in a common bath using two voltage clamps. The combined currents from the two oocytes cause problems because the bath clamp headstage cannot separate the individual currents and therefore cannot provide effective clamping.

OC-725C has two features to address these problems:
• An internal switch permits measurements of the current in series with the current electrode instead of in the bath.
• Optional differential voltage headstages (7255 DI): The differential measurement subtracts the voltage drop across the series resistance in the bath, which is normally eliminated by the bath clamp.


Specifications Back to Top

Specifications640028
Test Conditions1. Model cell used to obtain specifications: model membrane, 1 M? in parallel with 220 nF, 1 M? current and voltage electrodes.
2. Noise measurements made with an 8-pole Bessel filter.
Voltage Recording Channel (Vm): V Probe Input Impedance0.5 x 1012 ?, 1 pF
Voltage Recording Channel (Vm): Output Resistance100 ?
Voltage Recording Channel (Vm): DC Offset±200 mV at input, var. from zero with 10 turn control, (20 mV/turn)
Voltage Recording Channel (Vm): Noise (0-10 kHz)3 µV RMS with input grounded 20 µV RMS with model cell
Voltage Recording Channel (Vm): Electrode Test10 mV/M? read on meter 100 mV/M? at Vm x10 output
Voltage Recording Channel (Vm): Meter Range±199.9 mV full scale
Voltage Recording Channel (Vm): Capacity Compensation0 - 90 pF
Current Sensing (Bath Clamp) Channel (Im): Noise (0-1 kHz)4.5 nA RMS with bath clamp
10 nA RMS in output leg
Current Sensing (Bath Clamp) Channel (Im): I Monitor Output1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10
Current Sensing (Bath Clamp) Channel (Im): Gain Telegraph Output0.2 to 2.6 V in 7 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software* *Axon pClamp and Instrutech Pulse
Current Sensing (Bath Clamp) Channel (Im): Meter Range, full scaleClamp Current: ±199.9 µA
Current Sensing (Bath Clamp) Channel (Im): Electrode Voltage Ve±199.9 mV (Current meter reads Ve when clamp mode switch is off)
Current Electrode Channel: Compliance Voltage±180 V
Current Electrode Channel: Clamp Speed350 µsec. (10-90%) with 100 mV square wave command applied to model cell
Current Electrode Channel: GainVariable AC/DC: 0 to 2000
Current Electrode Channel: Fixed DC GainSwitch selected: 1 x 106
Current Electrode Channel: Ve DC Offset±200 mV at input (20 mV/turn)
Current Electrode Channel: Electrode Test10 mV/M? read on current meter
100 mV/M? at Ve x10 output, rear panel
Commands: HoldManually set with digital potentiometer, 2 digit resolution and 2 ranges:
x1 range: ±1 to 99 mV in 1 mV steps
x2 range: ±2 to 198 mV in 2 mV steps
Commands: ExternalSignals applied to COMMAND IN÷10 are attenuated by a factor of 10, 1 V applied = 100 mV command
Power Requirements100-130 V or 220-240 VAC, 50/60 Hz, 20 VA
Case Height Metric8.9 cm
Case Width Metric43.2 cm
Case Depth Metric30.5 cm
Bath Headstage Height Metric2.3 cm
Bath Headstage Width Metric3.5 cm
Bath Headstage Length Metric4.2 cm
Voltage Headstage Depth Metric12.5
Voltage Headstage Length Metric5
Mounting Handle Depth Metric4.8
Mounting Handle Length Metric6.3
Shipping Weight Metric6.8 kg
WarrantyThree years, parts and labor

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Journal Articles Back to Top

Displaying 1 to 10 of 11results for OC-725C Oocyte Clamp Amplifier   Page 1  2 

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Document

Description

100% Login to view WR11 Oocyte Clamp Amplifier.pdf
[453 KB]
Oocyte Clamp Amplifier use in finding 4-Methylhomoibotenic Acid Activates a Novel Metabotropic Glutamate Receptor Coupled to Phosphoinostide
36% Login to view 4-Methylhomoibotenic Acid Activates a Novel Metabotropic.pdf
[453 KB]
Oocyte Clamp Amplifier use in finding 4-Methylhomoibotenic Acid Activates a Novel Metabotropic Glutamate Receptor Coupled to Phosphoinostide
29% Login to view WR20 OC725C.pdf
[168 KB]
Inactivation of Kv2.1 Potassium Channels with use of Voltage-Clamp Amplifier
28% Login to view Cation Selectivity of Gastric H,K-ATPase.pdf
[199 KB]
Oocyte Clamp use in finding Cation Selectivity of Gastric H,K-ATPase and Na,K-ATPase Chimeras
26% Login to view Inactivation of Kv2.1 Potassium Channels.pdf
[183 KB]
Inactivation of Kv2.1 Potassium Channels with use of Voltage-Clamp Amplifier
18% Login to view WR08 OC725 Amplifier.pdf
[192 KB]
Oocyte Clamp use in finding Cation Selectivity of Gastric H,K-ATPase and Na,K-ATPase Chimeras
13% Login to view Activation of calcium entry in human carcinoma.pdf
[199 KB]
Patch Clamp Amplifier use in Activation of calcium entry in human carcinoma A431 cells
13% Login to view WR15 PC501A Patch Clamp.pdf
[193 KB]
Patch Clamp Amplifier use in Activation of calcium entry in human carcinoma A431 cells
10% Login to view Functional and Biochemical Evidence for Heteromeric.pdf
[208 KB]
OC-725B Amplifier use in finding Functional and Biochemail Evidence for Heteromeric ATP-gated Channels Composed on P2X1 and P2x5 Subunits
10% Login to view A Protein Kinase C Site Highly Conserved in P2X Subunits.pdf
[331 KB]
OC-725B amplifier use in finding A Protein Kinase C Site
   

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Manuals Back to Top

Displaying 1 to 1 of 1result for OC-725C Oocyte Clamp Amplifier  

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100% Login to view 600123 temp amp.pdf
[75 KB]
Temperature amplifier manual
   

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