Julio E. Celis. This comprehensive handbook is likely to become the standard laboratory manual in cell biology. The second addition contains four volumes in a plastic-comb bound to lie flat. The first volume deals with tissue culture and associated techniques and discusses viruses. The second volume is dedicated to organelles and cellular structures, assays, antibodies, immunocytochemistry, cell staining, and internet resources. Volume three describes light microscopy and contrast generation, electron microscopy, intracellular measurements, cytogenetics and hybridization, transgenics and gene knockouts. The last volume completes the set by describing topics such as transfer of macromolecules and small molecules, expression systems, differential gene expression, and proteins. (2400 pp; 1997)