Juan C. Lacal. This detailed book presents information on the principles of microinjection. It also covers the required equipment, preparation of receiving cells and injected material, and data analysis. Detailed protocols are provided for several applications in different cell systems and including cell cycle regulation, signal transduction, transcriptional regulation, cytoskeletal functions, and cell secretion and intracellular transport. A large portion of the book is also dedicated to the very relevant area of the Xenopus laevis oocyte system. (approx. 350 pp; 1999)