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tissue bath systems
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Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
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The Mayflower tissue bath is a horizontal tissue chamber with an integral
contraction force measurement. It is completely open at the top and has a
small bath volume of 3 - 5 ml. The compact and modular construction pro-
vides ideal conditions for investigations on small muscle preparations
(urethra, papillary muscle, cavernous body), vascular rings, or tracheal
rings.
Basic Equipment:
Acrylate support with a movable mounting platform for preload
adjustment through a micrometer screw. This platform receive
the tissue chamber and acts as holder for the force transducer.
The acrylate support also includes a glass heat exchanger for
the pre-heating of the perfusate solution.
The tissue chamber differentiates the two versions.
Flow Through System (73-2155)
The tissue chamber is carved out of an acrylate block and placed on the
support stand. This chamber includes the tissue holder, the connections for
electrical stimulation electrodes, a frit for aeration and a draw-off tube
with height adjustment to set the bath volume. As it is not jacketed,
continuous flow through the chamber is required.
Horizontal tissue bath for isometric contraction
measurements using a F10 or F30 force transducer
Two versions are available:
Flow-through superfusion system
Incubation system
Possibility of electrical stimulation
Exchangeable holders depending on tissue used
(for vascular or tracheal rings, for strips)
Incubation System (73-3600)
The tissue chamber is jacketed. The bottom of the chamber is made of a
teflon coated stainless steel plate to optimize the temperature stability of
the solution. A additional small stainless steel heat exchanger is also built
in to avoid temperature fluctuation.
The incubation chamber can also be used as a flow-through superfusion
chamber. The superfusion chamber cannot be used for incubation.
Additional Equipment:
A multi-channel roller pump for the perfusate circuit
A thermocirculator for keeping the perfusate solution
at constant temperature
The modular concept of this apparatus offers a wide range of different
arrangements to meet individual requirements regarding bath geometry,
tissue holders and stimulation electrodes. The horizontal arrangement of
the tissue and the open top provide ideal conditions during preparation
and experiment.
The various parts of the apparatus are made mainly from Plexiglas or
stainless steel and are therefore inert and corrosion-resistant. The solution
flows in from a roller pump and passes through a heat exchanger, the
outflow is under suction through a suction tube with height adjustment by
the same pump. A frit for aeration is located in the tissue chamber. Two
connection sockets for the stimulation electrodes are located on either
side of the tissue chamber.
Mayflower Tissue Bath System
73-2155 Mayflower Tissue
Bath Configured as a flow-
through system
Mayflower Tissue Bath System
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