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isolated organ systems
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Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
Order # Product
73-2158 Horizontal Tissue Bath PBTO, Type 813/6
73-2044 Device for Afterload Pressure of 0-30 cmH20
73-2333 Device for Afterload Pressure of 0-300 mmHg
Benefits and Features
Perfusion of tubular organs (trachea, blood
vessels, intestines)
Individual solutions for intraluminal perfusions
and extraluminal superfusion
Controlled perfusion pressure
Applications
Intraluminal perfusion of tubular organs (trachea,
blood vessels, intestines)
Testing circular-action musculature, vascular tone
and stents in isolated vessels
PBTO
The PBTO has been designed for the studying of perfused tubular organs
such as trachea, blood vessels, intestines and vas deferens. Individual
solutions can be used for intraluminal perfusion and extraluminal
superfusion. Two peristaltic pumps are required, one for intraluminal
constant flow perfusion and the second for extraluminal superfusion. The
intraluminal perfusion pressure is generated by an adjustable afterload
control system. A differential pressure transducer is used to measure the
intraluminal pressure difference at the proximal and distal end of the organ.
The tissue bath is a jacketed Plexiglas bath. The holder for the cannulae
can be removed from the main bath for the cannulation of the segment of
tubular organs. The cannulae are fixed on sliding holders to adjust to
organs of different length, up to 50mm. The intraluminal pressure is
controlled by one of two afterload systems available. One for low pressure
Applications
Basic test in gastro-intestinal research
Peristaltic reflex in guinea pig ileum
For studying emptying phase of peristaltic reflex
IPR
The IPR (Ileum Peristaltic Reflex) is a version of the PBTO that has been
designed for studying the peristaltic reflex in the guinea-pig ileum. The circular
muscle contraction which propels the contents of the ileum from aboral to
anal direction is induced by the increase of the intraluminal pressure up to a
threshold point.
The tissue bath is a jacketed Plexiglas bath. The holder for the cannulae can
be removed from the main bath during the preparation. The oral and aboral
ends of a 4 to 5 cm long segment of guinea pig ileum is cannulated. Three
different sizes of cannulae are available. The cannulae are fixed on sliding
holders to adapt the distance between the cannulae to the individual length of
the ileum. The inflow is controlled by means of a peristaltic pump with a
flowrate of about 0.5 ml/min. The direction of the perfusion flow must meet
the physiological way. The outflow can be switched using a stopcock from a
level equivalent to the level of the bath to a level of about 3 cm above the
level of the fluid in the bath. The peristaltic reflex is induced by this procedure.
In the `peristalsis' position, the pressure increases slowly resulting in a
distention of the ileum until the pressure threshold level is reached. At this
time the emptying phase is started as a visible contraction of the circular
muscle moving from the oral to the aboral side.The intraluminal pressure is
then recorded from the oral side.
Additional equipment required: thermocirculator, reservoir, peristaltic pump,
transducer, monitoring system setup using the PLUGSYS Amplifier System.
Recording and Evaluation of the signals using BDAS
software.
Order # Product
73-3013 Horizontal Tissue Bath IPR, Type 813/2
applications (0 to 30 mmHg) and the second for high pressure applications
(0 to 300 mmHg).
The unit comes with four different interchangeable cannulae with the
following diameters: 1.5, 2.5, 3 and 4 mm. For smaller vessels customized
stainless steel cannulae can be made on request. For micro-vessels, glass
capillary pipettes pulled to the required diameter can be connected to the
adapter cannulae using a silicone tube collar.
A special sliding block for testing stent (e.g. drug diluting stent) has been
developed. An additional port allows insertion of a stent into a cannulated
isolated vessel.
Additional equipment required: thermocirculator, reservoir, peristaltic
pump, transducer, monitoring system setup using the PLUGSYS Amplifier
System. Recording and Evaluation of the signals using BDAS software.
Perfusion Bath for Tubular Organs (PBTO)
& IPR Bath for Ileum Peristalic Reflex
advanced
research
Standard PBTO Bath