such as trachea, blood vessels, intestines and vas deferens. Individual
solutions can be used for intraluminal perfusion and extraluminal
superfusion. Two peristaltic pumps are required, one for intraluminal
constant flow perfusion and the second for extraluminal superfusion. The
intraluminal perfusion pressure is generated by an adjustable afterload
control system. A differential pressure transducer is used to measure the
intraluminal pressure difference at the proximal and distal end of the organ.
can be removed from the main bath for the cannulation of the segment of
tubular organs. The cannulae are fixed on sliding holders to adjust to
organs of different length, up to 50mm. The intraluminal pressure is
controlled by one of two afterload systems available. One for low pressure
designed for studying the peristaltic reflex in the guinea-pig ileum. The circular
muscle contraction which propels the contents of the ileum from aboral to
anal direction is induced by the increase of the intraluminal pressure up to a
be removed from the main bath during the preparation. The oral and aboral
ends of a 4 to 5 cm long segment of guinea pig ileum is cannulated. Three
different sizes of cannulae are available. The cannulae are fixed on sliding
holders to adapt the distance between the cannulae to the individual length of
the ileum. The inflow is controlled by means of a peristaltic pump with a
flowrate of about 0.5 ml/min. The direction of the perfusion flow must meet
the physiological way. The outflow can be switched using a stopcock from a
level equivalent to the level of the bath to a level of about 3 cm above the
level of the fluid in the bath. The peristaltic reflex is induced by this procedure.
In the `peristalsis' position, the pressure increases slowly resulting in a
distention of the ileum until the pressure threshold level is reached. At this
time the emptying phase is started as a visible contraction of the circular
muscle moving from the oral to the aboral side.The intraluminal pressure is
then recorded from the oral side.
transducer, monitoring system setup using the PLUGSYS Amplifier System.
Recording and Evaluation of the signals using BDAS
(0 to 300 mmHg).
following diameters: 1.5, 2.5, 3 and 4 mm. For smaller vessels customized
stainless steel cannulae can be made on request. For micro-vessels, glass
capillary pipettes pulled to the required diameter can be connected to the
adapter cannulae using a silicone tube collar.
developed. An additional port allows insertion of a stent into a cannulated
pump, transducer, monitoring system setup using the PLUGSYS Amplifier
System. Recording and Evaluation of the signals using BDAS software.
& IPR Bath for Ileum Peristalic Reflex