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tissue bath systems
17
Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
TIOX Tissue Bath for O
2
Consumption
& Contraction Force Measurement
advanced
research
Benefits and Features
Unique sealed system to measure muscle
contraction and oxygen consumption.
Oxygen electrode with very low rate of oxygen
consumption
Small volume
Applications
Bioenergetics studies
Sarcopenia in senescence studies
Evaluation of metabolic - contractile caracteristics
Fatigue and muscle weakness studies
Sports physiology and biochemistry
Order # Product
73-3794 TIOX Tissue Bath
69-3006 Microcathode Oxygen Electrode
72-1975 Magnetic Mini Stirrer
72-1977 Micro Stirring Bar
73-0831 HSE Isometric Force Transducer F30
The TIOX tissue bath is intended for experiments on isolated tissue preparations
by measuring simulanously the tissue contraction and the oxygen consumption. It
is used in fundamental research in physiological and pharmacological
laboratories. It is particularly suitable for experiments in sport physiology.
The TIOX tissue bath consists of a Plexiglass baseplate with a vertical column.
It carries a movable platform holding the tissue support and the transducer
for measuring the contractile force. The tissue vessel is also mounted on the
platform and is movable, it includes the Clark pO
2
electrode for measurement
of oxygen consumption. Fresh solution which must be pre-warmed and aerated
is introduced into the vessel with a syringe from the bottom of the vessel.
The tissue support is suitable for mounting muscle strips of a maximum
length of 20mm (0.75 inch) and is equiped with two parrallel plate electrodes
made of platinum for electrical field stimulation.
The following items and equipment are required for work with the tissue bath:
Thermocirculator with approx. 3 liter capacity
Magnetic stirrer
Reservoir for perfusion solution with frit for equilibrating the
solution supply
Transducer to measure the muscle contractions (isometric or
isotonic as required) with vernier control for fine adjustment of
the pretentioning
A bridge amplifier for the transducer, for signal conditioning
OPPM amplifier for the Clark pO
2
electrode, for signal conditioning
and adaptation to a data acquisition system
Data acquisition system
1. Tissue Vessel
2. Tissue Holder
3. Clark Type pO
2
Electrode
4. Temperature Probe
5. Drug Injection Line
6. Draining and Refilling Line
7. Tissue Hook with Transmission
Wire to the Force Transducer
8. Fixing Hook for Mounting the Tissue
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