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tissue bath systems
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Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
preparation. The lower part is funnel-
shaped to allow the collection of the
efluate for reperfusing, perfusing the
next tissue or bioassay analysis. The
isometric force transducer is fixed on
the rod using a Vernier control.
The tissue holder is a separate part that
is installed in the main superfusion
chamber. The holder can easily be
removed to simplify the preparation.
The holder consists of the body, the
hook for fixing the tissue, the stainless
steel cannulas for superfusion and the
stimulation electrodes. The tissue is
attached using a thread to the tissue
hook. The thread at the other end of the
tissue must be attached to the
transducer. A slide in the holder body
allows easy positioning of the thread in
the central hole. The superfusion
cannulae can be rotated in the main
body to optimize the superfusion after the holder is in place into the chamber
and the thread fixed to the transducer. The stimulation electrodes are made of
platinum and must be buckled to be close to the tissue. The electrodes do not
touch the tissue but must be parallel and close enough to the tissue so that the
superfusing solution makes electrical contact.
The dual heat exchanger consists of Tygon tubing loops inside a jacket
tube connected to a thermocirculator.
In the Flow through mode each tissue is superfused individually with fresh
solution or solution containing the test compound. The outcoming solution is
collected and goes to waste or is stored for later bioassay analysis.
In the Recirculating mode each tissue is superfused individually. The
outcoming solution after superfusion is collected and used for superfusing
again in the same tissue.
In the Cascade mode the first tissue is superfused with fresh solution and
all the subsequent tissues are superfused with the outcoming solution of
the preceding chamber.
Additional equipement required: peristaltic pump, force transducer with
Vernier control, bridge amplifier for the force transducer, Data acquisition
ACAD, electrical stimulator, thermocirculator if heat exchangers are used.
The Coleman superfusion system is suitable for a variety of applications
involving the study of contraction and relaxation of smooth muscle
preparations, the effect of drugs that interfere with these actions, as well as
those that interfere with autonomic neurotransmission. The system consists
of a main stand that can receive up to eight single superfusion chambers.
Each chamber is equipped with electrical stimulation capability, a rod for
fixing the force transducer and an optional two channel heat exchanger. The
lower end of the tissue is held by a stainless steel hook. The thread attached
to the top of the tissue passes through the opening directly to the force
transducer. The superfusion solution is fed by a peristaltic pump through a
stainless steel cannula to the thread; it runs down the thread and superfuses
the tissue. Two separate solution paths are available for superfusion with
and without test substance. Two platinum wires are placed parallel to the
tissue to form stimulation electrodes. The solution running down provides
electrical contact between the electrodes and the tissue.
The superfusion chambers are made of Plexiglas and do not require
thermostating owing to the low thermal conductivity of the material. The
chamber is fixed on the frame in the horizontal position. All additional parts
(tissue holder, dual heat exchanger, road for mounting the transducer) are
fixed on the chamber. The upper part of the chamber receives the tissue
holder. The lower part can be swung away to simplify access to the
Vernier
Control
Force
Transducer
Superfusion
Lines
Tissue
Hook
Stimulation
Electrodes
Order # Product
73-2221 Basic Unit for Superfusion Bath according to
COLEMAN (stand for 4-Baths) Type 840
73-2222 Superfusion Bath according to COLEMAN
73-2917 Heat Exchanger to Superfusion Bath COLEMAN
Coleman
Superfusion
Bath
Benefits and Features
Suitable for virtually any tissue sample
Compact, allows the study of up to four tissue
preparations in parallel
Rapid removal of potentially toxic metabolites
Obviates the necessity of repeated washing
procedures
Simple reclaimation of perfusate for recirculation
or collection
Applications
Evaluation of the potency of labile substances
Determining the rates of onset and offset of
drug action
Evaluation of spasmogenic and spasmolitic agents
For a system description according your requirements please use:
www.hugo-sachs.de/timail.html
or contact our technical experts at:
physiology@harvardapparatus.com
For a custom configuration and full system quotation.
Coleman Superfusion Bath
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