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moist chambers
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Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
Moist Chamber Type 834/8
Order # Product
73-2901 Moist Chamber Type 834/8 with Metal Tube
Heat Exchanger
73-3692 Bubble Trap for Flow Rate up to 50 ml/min
73-3094 Stainless Steel Mesh Electrode
Mesentric Bed Per-
fusion System
Benefits and Features
Excellent temperature control for perfusate and organ
Precise positioning of cannulae and measurement
probes
Straight forward operation and compact dimensions
A large choice of cannulae, bubble traps and other
acessories makes the moist chamber suitable for a
huge range of perfusion applications
Provides a complete perfusion system in combination
with the UP-100 or Perfusion Control System
Applications
For perfusion of organs from rodents like liver,
kidney, pancreas and mesenteric bed
For investigating the tone of small blood vessels
under the effect of vasoactive substances
Biochemistry, studying metabolic processes
Moist chamber equiped for isolated
rat liver perfusion under constant
flow conditions
Mini ball joint holders support
arterial and venous cannulae for
rat liver perfusion
Moist Chamber
73-2901
The popular Moist Chamber Type 834/8 is an exceptionally flexible and
useful tool for perfusion of most "simple" organs from typical rodent
models. In its most basic configuration, the Moist Chamber consists of a
suitably deep (110x40x35mm) organ chamber and tight-fitting cover. Both
components are double-walled and water-jacketed to provide a stable
temperature controlled environment within the organ chamber. Perfusate
is warmed by passage through a built-in heat exchanger and bubble trap
immediately before contact with the organ.
Inside the chamber, a flexible silicon platform acts as a rest for fixation
(usually with standard fixing pins) of the organ. Anchors for our Mini Ball
Joint positioning system and precision arterial and venous cannulae are
pre-drilled on both sides of the organ. In addition, several measurement
and sample ports are provided for easy access to the inner chamber, even
with the cover in place, making the chamber suitable for collecting a wide
range of physiological data.
The Moist Chamber Type 834/8 can be part of a simple constant flow
perfusion system. Used as such, a water-jacketed buffer reservoir, peristaltic
pump and appropriate cannulae are used to complete the perfusion circuit,
while a thermocirculator feeds the water-jacketed components to maintain
the thermostating circuit. The Moist Chamber Type 834/8 can also be used to
form the core of our UP-100 or Perfusion Control system to permit perfusion at
constant pressure as well as constant flow.
The basic Moist Chamber Type 834/8 is configured as shown above and
consists of the chamber, cover and silicon plate only. Contact Harvard
Apparatus for configuration of a complete perfusion system for specific
applications.
Additional equipment required: thermocirculator, bubble trap, cannulae,
holders, peristaltic pump, transducers, monitoring system setup using the
PLUGSYS Amplifier System. Recording and Evaluation of the signals using
BDAS software.
Special Application: The Rat Mesenteric Bed
The key part of the perfusion system for the rat mesenteric bed is the moist
chamber. The mesenteric tissue is placed into the moist chamber on a
stainless steel mesh (replaces the silicone plate) which also acts as anode
during electrical stimulation.
advanced
research
For a system description according your requirements please use:
www.hugo-sachs.de/orgmail.html
or contact our technical experts at:
physiology@harvardapparatus.com
For a custom configuration and full system quotation.