pump, transducer for perfusion pressure, servo controller (SCP), monitoring
system setup using the PLUGSYS Amplifier System. Recording and Evaluation
of the signals using BDAS software.
weighing during perfusion.
can be customized for other types of organ to be perfused.
and Universitätsklinikum, Chirurgie, 24105 Kiel.
pressures and bowel weight.
system. The jacketed chamber maintains a warm and moist environment for
the organ. The chamber has been configured for studying the edema evolution
in a perfused intestine with attached mesenteric bed with two separate
perfusion lines for simultaneous vascular and intraluminal perfusion.
required heating coils and bubble traps. This block acts also as holder for the
tubing and cannulae, it can be placed near the animal for easy in-situ
preparation. After surgery, the block with the preparation is moved and fixed
on the chamber. This ensures continuous perfusion during the entire duration
of surgery and reduced risk of embolism or ischemia.
system and perfusate collection. Measurement of perfusion pressures and
flows are also available. A peristaltic pump is used for vascular perfusion. A
constant pressure is maintained by controlling the pump speed with an
electronic controller (SCP) via pressure measurement. For the intraluminal
Ileum perfusion, a syringe pump is used.
fen Bade,2 Holger Schultz,2 Peter Zabel,2 Jens Scholz,3 Norbert Weiler,3,* and Stefan Uhlig2
Hospital Schleswig-Holstein, Campus Kiel
are needed. It was our aim to develop a long term stable isolated perfused rat small bowel prepara-
tion permitting analysis of vascular, luminal, interstitial and lymphatic compartments and to demon-
strate the utility of this model by studying the effects of the pro-inflammatory mediator
platelet-activating factor (PAF). A temperature-controlled chamber with an integrated balance was
or oxygen carriers were not needed. Functional and morphological integrity of the tissue was pre-
served for several hours as confirmed by oxygen consumption, venous lactate-to-pyruvate ratio,
arterial and venous pH, lactose digestion and galactose uptake, intravascular and luminal pres-
sures, maintained fluid homeostasis, gut motility, and by quantitative light microscopic analysis.
Administration of PAF caused typical effects such as vasoconstriction, gut atony, and loss of
galactose uptake. PAF also elicited a transient loss of 20% of the perfusate liquid from the mesen-
teric vascular bed, two thirds of which were transferred to the lumen. All these responses were en-
tirely reversible. This new model provides detailed insights into the physiology of the small
intestine, and will allow to study fundamental processes such as fluid homeostasis, barrier func-
tions, transport mechanisms and immune responses in this organ. Using this model, here we
show a dramatic and yet reversible response of the rat small bowel to PAF suggesting luminal
water clearance as a novel safety factor in the intestine that may be of clinical relevance. Intes-
tine physiology; fluid balance; platelet activating factor.