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moist chambers
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Harvard Apparatus · phone 508.893.8999 · toll free U.S. 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com
Additional equipment required: thermocirculator, peristaltic pump, syringe
pump, transducer for perfusion pressure, servo controller (SCP), monitoring
system setup using the PLUGSYS Amplifier System. Recording and Evaluation
of the signals using BDAS software.
The chamber can be used for any other organ that requires continuous
weighing during perfusion.
The interchangeable adapter block holding the perfusion lines and cannulae
can be customized for other types of organ to be perfused.
Developed in cooperation with the Forschungszentrum Borstel, 23845, Borstel
and Universitätsklinikum, Chirurgie, 24105 Kiel.
· Compact arrangement
· Dual Perfusion System,
vascular & intraluminal
intestinal
· Built-in balance for edema
evaluation/organ
weight measurement
· Vascular bed is in warm
and moist environment
during perfusion
· Optimized temperature
control of perfusate and
organ
· Controlled perfusion
conditions
· Cannulation block simplifies surgery
Applications
· To study simultanuously vascular, luminal and
lymphatic flows, arterial, venous and intraluminal
pressures and bowel weight.
· Septic multi-organ failure study in gastro intestinal area
Moist Chamber with Edema Balance
Moist Chamber for Microvascular Permeability Studies
The system is based on a moist chamber with a built-in organ weighing
system. The jacketed chamber maintains a warm and moist environment for
the organ. The chamber has been configured for studying the edema evolution
in a perfused intestine with attached mesenteric bed with two separate
perfusion lines for simultaneous vascular and intraluminal perfusion.
The chamber is supplied with a movable cannulation block including all the
required heating coils and bubble traps. This block acts also as holder for the
tubing and cannulae, it can be placed near the animal for easy in-situ
preparation. After surgery, the block with the preparation is moved and fixed
on the chamber. This ensures continuous perfusion during the entire duration
of surgery and reduced risk of embolism or ischemia.
The chamber provides a number of ports for connecting the measurement
system and perfusate collection. Measurement of perfusion pressures and
flows are also available. A peristaltic pump is used for vascular perfusion. A
constant pressure is maintained by controlling the pump speed with an
electronic controller (SCP) via pressure measurement. For the intraluminal
Ileum perfusion, a syringe pump is used.
Order # Product
73-3685 Moist Chamber with Edema Balance Type 802 (MCWEB)
Cannulating block
Weighing
Transducer
Vascular
Cannulae
Intraluminal
Cannulae
Cannulation block removed
from chamber for surgery
73-3685
advanced
research
Citations
A Model of the Isolated Perfused Rat Small Intestine
Ingmar Lautenschläger,1 Heike Dombrowsky,2 Inez Frerichs,3 Solveig-Carolin Kuchenbecker,3 Stef-
fen Bade,2 Holger Schultz,2 Peter Zabel,2 Jens Scholz,3 Norbert Weiler,3,* and Stefan Uhlig2
1University Medical Centre Schleswig-Holstein, Campus Kiel 2Research Center Borstel 3University
Hospital Schleswig-Holstein, Campus Kiel
Submitted 31 July 2009 ; revision received 8 October 2009 ; accepted in final form 8 November 2009
ABSTRACT
Intestinal edema remains a serious clinical problem and novel approaches to study its pathophysiology
are needed. It was our aim to develop a long term stable isolated perfused rat small bowel prepara-
tion permitting analysis of vascular, luminal, interstitial and lymphatic compartments and to demon-
strate the utility of this model by studying the effects of the pro-inflammatory mediator
platelet-activating factor (PAF). A temperature-controlled chamber with an integrated balance was
designed to perfuse isolated intestines through the mesenteric artery and the gut lumen. Steroids
or oxygen carriers were not needed. Functional and morphological integrity of the tissue was pre-
served for several hours as confirmed by oxygen consumption, venous lactate-to-pyruvate ratio,
arterial and venous pH, lactose digestion and galactose uptake, intravascular and luminal pres-
sures, maintained fluid homeostasis, gut motility, and by quantitative light microscopic analysis.
Administration of PAF caused typical effects such as vasoconstriction, gut atony, and loss of
galactose uptake. PAF also elicited a transient loss of 20% of the perfusate liquid from the mesen-
teric vascular bed, two thirds of which were transferred to the lumen. All these responses were en-
tirely reversible. This new model provides detailed insights into the physiology of the small
intestine, and will allow to study fundamental processes such as fluid homeostasis, barrier func-
tions, transport mechanisms and immune responses in this organ. Using this model, here we
show a dramatic and yet reversible response of the rat small bowel to PAF suggesting luminal
water clearance as a novel safety factor in the intestine that may be of clinical relevance. Intes-
tine physiology; fluid balance; platelet activating factor.