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isolated organ systems
25
Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
UP-100 Equipped
with Moist
Chamber
The UP-100 is a multi-purpose perfusion system best utilized when different
types of organs must be perfused either in situ or ex vivo. The modular design
of this system allows easy adaptation to different applications using additions
or extensions to the base unit.
System Extensions for Perfusion Ex-Vivo
Internal organs (kidney, liver, mesenteric bed) must obviously be kept under
optimal physiological conditions; moist and at defined temperature during
perfusion. For these applications the UP-100 is combined with the Moist
Chamber Type 834/8.
System Extensions for Perfusion in Situ
For in situ perfusion of organs such as liver and kidney, or for perfusion of
regional vascular systems like hindquarter, an operating table can be placed on
the main Plexiglas plate below the UP-100 mounting platform. The compact
arrangement allows the connection line between organ and heat exchanger to
remain short to ensure consistent perfusate temperature.
System Extensions for Perfusion of Mouse, Rat,
Guinea-pig or Small Rabbit Heart
For perfusion of the isolated heart according to Langendorff, the UP-100 can be
equipped with a jacketed heart chamber and additional measurement system
for isovolumetric LVP.
Additional equipment required: thermocirculator, electrical stimulator, peristaltic
pump, transducer for perfusion pressure, monitoring system setup using the
PLUGSYS Amplifier System. Recording and Evaluation of the signals using
BDAS software.
Multi-purpose system for perfusing isolated
organs in-situ or ex-vivo
Ideal for perfusing isolated organs such as:
Liver Rabbit Ear
Heart
Kidney Rat hind limb
Mesenteric bed
Perfusion can be performed at constant pressure
or constant flow without involving any
modification of apparatus
Ideal replacement for perfusion system with hydrostatic
pressure generation, low perfusate volume in use,
perfusion pressure up to 300 mmHg possible
Can be equipped with an oxygenator for optimal
aeration of perfusate containing albumin or
erythrocytes
Applications
In situ perfusion of hind limb, hindquarter
mesenteric bed, liver, kidney:
Blood vessel tone in peripheral vascular bed
Balance tests by muscle work
(glucose/lactate/pyruvate, high energy
phosphates/orthophosphate, etc.)
Test of vasodilative drugs in occlusive
diseases of legs
Test of muscle relaxants (end-plate pharmacology)
Ex-vivo perfusion of liver, kidney, mesenteric bed,
by using the additional Moist Chamber Type 834/8
Test of vasodilative drugs
Studying metabolic processes
Neural vascular tone
Organ preservation for transplant
Special version for Langendorff heart perfusion
Optimized for LVP measurement
Ideal for compound screening
Order # Product
73-2316 Universal Perfusion System Basic Unit UP-100, Type 834
The UP-100 is a universal system. It needs to be adapted to the specific application by adding
other equipment.
UP-100 Equipped with
Heated Surgery Table
For a system description according your requirements please use:
www.hugo-sachs.de/orgmail.html
or contact our technical experts at:
physiology@harvardapparatus.com
For a custom configuration and full system quotation.
Universal Perfusion System UP-100
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