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isolated heart systems
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Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
IH-5 Isolated Heart Size 5
Rat, Guinea Pig and Rabbit (contined)
Specifications
Perfusion Kit
Body Weight Up to 2.5 kg
Heart Diameter 25 mm
Heart Length (Base - Apex) 30 mm
Aorta, ID Up to 5 mm
Aortic Flow (in WH Mode) Up to 500 ml/min
Perfusion Pressure (Afterload) Up to 300 mmHg
Order # Product
73-3604 Basic Unit for Isolated Heart Size 5 Type 843/1
73-2916 Heart Chamber for IH5 D=135/100 mm H=115 mm
73-3072 Aortic Cannulae Set to IH-5 for Rat/Guinea Pig Hearts
73-0719 Aortic Cannulae Set to IH-5 for Rabbit Hearts
73-3064 Working Heart Option to IH-5 Basic Unit
73-3066 Left Atrium Cannula for IH-5 for Rat/Guinea Pig Heart,
OD=2.3 mm
73-2880 Left Atrium Cannula for IH-5 for Rabbit Heart, OD=6.0 mm
System Description
The IH-5 is very compact, improved for low volume and high environment
stability.
All of the components are mounted on a basic Plexiglas stand except for the
thermocirculator, and the PLUGSYS amplifier system. Special attention has
been made to ensure that all critical connecting tubing are maintained short,
thus avoiding any cooling through a temperature gradient to ambient
temperature.
The key part of the system is the unique aortic block which is the interface
between the heart and the IH-5. The bubble trap, compliance chamber,
patented flow resistance membrane, infusion and measurement ports are all
integrated into this single specialized component. In addition, the aortic block
features adjustable attachment points for our proprietary Mini Ball Joint Holder
positioning system. This permits precise placement of measurement probes
for ultra-stable recordings.
The modular nature of the IH-5 allows the system to evolve along with your
research. A Langendorff system can easily be upgraded to full Working Heart;
and any IH-5 can be upgraded with special purpose capabilies like multi-
channel ECG and MAP for a complete cardiac electrophysiology workstation.
The connection to the heart is made through interchangeable aortic cannulae
and atrial cannulae in working heart extension. The perfusate is aerated by
bubbling in the reservoir. When the perfusate contains erythrocytes or albumine,
a special membrane oxygenator to prevent foaming can equip the unit. In the
Langendorff mode, heart contractility is measured using a balloon inserted
into the left ventricle and connected to a pressure transducer.
Additional equipment required: thermocirculator, peristaltic pumps, transducers,
monitoring system setup using the PLUGSYS Amplifier System. Data acquisition
and Evaluation using ISOHEART software.
advanced
research
Multi-Lead ECG Recording
The heart is immersed in the heart chamber. The ECG-electrodes are mounted
on an insert placed into the heart chamber. The ECG-signals are picked up from
the heart surface using the conductivity of the perfusate. The three electrodes
are arranged on the insert to mimic an `Einthoven' derivation with the reference
electrode located beneath the heart. The additional six electrodes are mounted
around the heart on a special ring to record unipolar Wilson leads V1 to V6.
The electrodes are connected to the input box located on top of the unit
(on the same box as the MAP electrodes). The box is connected to the PLUGSYS
EGM (Einthoven Goldberger Module) and the PLUGSYS WLA (Wilson Lead
Amplifier). The twelve leads recorded are I, II, III, aVL, aVR,
aVF, V1 to V6.
If only a single ECG derivation (II) is required two single elctrodes are simply
mounted with ball joint holders on the aortic block. In this case the insert is
not required.
Multiple MAP Recording
A special holder for up to 8 MAP-electrodes has been designed. On this holder
are located eight evenly spaced cantilever arms holding spring-loaded
monophasic action potential electrodes. The spring-force (pressure of the
electrodes against the epicardium) can be adjusted individually for each
electrode. Even the position of the electrode on the heart can be adjusted
individually. The electrodes are connected to the main input box type 701/1
fixed on the top of the unit. This input box is connected to the MAPM
(Monophasic action potential module) type 703 an especially designed PLUGSYS
module. One MAPM can handle six electrodes, two modules are requested.
A special electrode for endocardial MAP is also available. This electrode is
mounted with ball joint holders on the aortic block
Circular Holder for Placement of MAP Electrodes and Multi Lead ECG