thermocirculator, and the PLUGSYS amplifier system. Special attention has
been made to ensure that all critical connecting tubing are maintained short,
thus avoiding any cooling through a temperature gradient to ambient
between the heart and the IH-5. The bubble trap, compliance chamber,
patented flow resistance membrane, infusion and measurement ports are all
integrated into this single specialized component. In addition, the aortic block
features adjustable attachment points for our proprietary Mini Ball Joint Holder
positioning system. This permits precise placement of measurement probes
for ultra-stable recordings.
research. A Langendorff system can easily be upgraded to full Working Heart;
and any IH-5 can be upgraded with special purpose capabilies like multi-
channel ECG and MAP for a complete cardiac electrophysiology workstation.
and atrial cannulae in working heart extension. The perfusate is aerated by
bubbling in the reservoir. When the perfusate contains erythrocytes or albumine,
a special membrane oxygenator to prevent foaming can equip the unit. In the
Langendorff mode, heart contractility is measured using a balloon inserted
into the left ventricle and connected to a pressure transducer.
monitoring system setup using the PLUGSYS Amplifier System. Data acquisition
and Evaluation using ISOHEART software.
on an insert placed into the heart chamber. The ECG-signals are picked up from
the heart surface using the conductivity of the perfusate. The three electrodes
are arranged on the insert to mimic an `Einthoven' derivation with the reference
electrode located beneath the heart. The additional six electrodes are mounted
around the heart on a special ring to record unipolar Wilson leads V1 to V6.
The electrodes are connected to the input box located on top of the unit
(on the same box as the MAP electrodes). The box is connected to the PLUGSYS
EGM (Einthoven Goldberger Module) and the PLUGSYS WLA (Wilson Lead
Amplifier). The twelve leads recorded are I, II, III, aVL, aVR,
aVF, V1 to V6.
mounted with ball joint holders on the aortic block. In this case the insert is
are located eight evenly spaced cantilever arms holding spring-loaded
monophasic action potential electrodes. The spring-force (pressure of the
electrodes against the epicardium) can be adjusted individually for each
electrode. Even the position of the electrode on the heart can be adjusted
individually. The electrodes are connected to the main input box type 701/1
fixed on the top of the unit. This input box is connected to the MAPM
(Monophasic action potential module) type 703 an especially designed PLUGSYS
module. One MAPM can handle six electrodes, two modules are requested.
mounted with ball joint holders on the aortic block