individuals who wish to isolate primary cells from organs of species in the
size range of mouse to guinea pigs. The system can be configured for
either In Situ or Ex Vivo organ perfusion.
protease solution for tissue disintegration. The perfusate for blanching is
warmed and oxygenated in separate buffer reservoir and supplied via the
peristaltic pump and warming coil. The protease solution is in a separate
reservoir oxygenated and stirred. For In Situ perfusion an operating table can
be placed just below the warming coil, for Ex Vivo application (heart, liver...)
the system can be equipped with a jacketed chamber. We offer a complete
line of custom perfusion cannula (Aortic, In Situ and Ex Vivo) to
accommodate vessels from 1.0 to 3.5 mm ID. The In Situ and Ex Vivo
cannulae feature our unique tip basket to prevent vessel occlusion.
for Cell Isolation
minimizes the bench space required for the system. The wetted
components of the perfusion circuit can be flushed with a 70% ethanol
solution or autoclaved for sterilization purposes.
magnetic stirrer, reservoir, cannulae, operating table and others depending
on verison selected, eventualy transducers, monitoring system setup using
the PLUGSYS Amplifier System.