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cell isolation
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Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
This perfusion system has been specifically created to meet the needs of
individuals who wish to isolate primary cells from organs of species in the
size range of mouse to guinea pigs. The system can be configured for
either In Situ or Ex Vivo organ perfusion.
The system is first used with perfusate for the blanching and than with the
protease solution for tissue disintegration. The perfusate for blanching is
warmed and oxygenated in separate buffer reservoir and supplied via the
peristaltic pump and warming coil. The protease solution is in a separate
reservoir oxygenated and stirred. For In Situ perfusion an operating table can
be placed just below the warming coil, for Ex Vivo application (heart, liver...)
the system can be equipped with a jacketed chamber. We offer a complete
line of custom perfusion cannula (Aortic, In Situ and Ex Vivo) to
accommodate vessels from 1.0 to 3.5 mm ID. The In Situ and Ex Vivo
cannulae feature our unique tip basket to prevent vessel occlusion.
Easy Set-up for Cell Extraction
Easy Setup for Cell Extraction by Constant
Flow Perfusion
Cell isolation from mouse, rat, guinea pig
organs by disintegration using protease solution
Designed for cardiomyocyte isolation
Can be used for other organs In Situ or Ex Vivo
Order # Product
73-3756 Easy Setup for Cell Extraction by Organ
Disintegration Type 803
73-3761 Heart Chamber to Easy Cell Extraction System
Perfusion System
for Cell Isolation
This system is simple to setup, operate and maintain. Its compact design
minimizes the bench space required for the system. The wetted
components of the perfusion circuit can be flushed with a 70% ethanol
solution or autoclaved for sterilization purposes.
Additional equipment required: thermocirculator, peristaltic pumps,
magnetic stirrer, reservoir, cannulae, operating table and others depending
on verison selected, eventualy transducers, monitoring system setup using
the PLUGSYS Amplifier System.
research
For a system description according your requirements please use:
www.hugo-sachs.de/ihmail.html
or contact our technical experts at:
physiology@harvardapparatus.com
For a custom configuration and full system quotation.