isolated lung systems
Harvard Apparatus · phone 508.893.8999 · toll free U.S. 800.272.2775 · fax 508.429.5732 · www.harvardapparatus.com
IPL-1 Isolated Perfused Lung for Mouse
Ventilation of the lung is possible under negative pressure as well as
under positive pressure (the latter is essential during the preparation
phase). The ventilation parameters are controlled with a special PLUGSYS
Ventilator, the "Ventilation Control Module" (VCM, Type 681).
The apparatus consists essentially of a flat jacketed Plexiglass thoracic
chamber and a jacketed cover. The size has been chosen so that a
complete mouse can be mounted inside it. The walls of the Plexiglass dish
carry all the auxiliary devices, connections and ports for perfusion and
ventilation. This arrangement offers the advantage that connecting tubing
and cannulae can be kept short, resulting in a very small dead volume and
ensuring optimum thermal stability.
Perfusion takes place at constant flow. The perfusate is passed by means
of a roller pump at constant flow through the heat exchanger, through a
bubble trap to the pulmonary artery and finally into the lung vascular bed.
The venous discharge is provided by cannulating the pulmonary vein, the
cannula is placed in the left atrium of the heart. The venous discharge
passes into the pressure balancing vessel (compensation for the vascular
transmural pressure) and is drawn off from there by the second channel of
the roller pump.
During the preparation phase the animal is ventilated at positive pressure.
After the connections of the trachea and the perfusion supply have been
made ventilation can be changed over to negative pressure. The chamber
now forms an artificial thorax.
Several adapters are available to work with standard ventilators (positive
pressure ventilation only), to supply different air mixtures to the lungs or to
connect a nebulizer for aerosol challenge.
Order # Product
73-4292 IPL-1 Core System, 115 V
73-4291 IPL-1 Core System, 230 V
VCM is a PLUGSYS module and requires a system maincase.
The Following Signals Can be Measured:
FL Respiratory Airflow
TCP Thoracal Chamber Pressure (intrapleural pressure)
TP Tracheal Pressure
PP Perfusion Pressure
VP Venous Outflow Pressure
a/e Oxygen Partial Pressure in the Affluent/Effluent
Partial Pressure in the Affluent/Effluent
pH a/e pH in the Affluent/Effluent
The Following Parameters Can be Evaluated Using
-Peak Inspiratory and Expiratory Airflow
-Tidal Volume, Minute Volume
-Dynamic Lung Resistance, Dynamic Compliance
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