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isolated lung systems
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Harvard Apparatus phone 508.893.8999 toll free U.S. 800.272.2775 fax 508.429.5732 www.harvardapparatus.com
IPL-16 Isolated Perfused Lung for
Pigs and Large Animals
Description
The apparatus consists essentially of a jacketed Plexiglass thoracic
chamber (inside dimensions 400 x 500 x 300 mm) and a jacketed cover.
The size has been chosen so that a complete pig lung (both lobes) can be
mounted inside it. The lung is placed on a platform. The platform can be
moved out of the chamber to facilitate the surgery. All connections for
perfusion and ventilation are mounted on the plattform. This arrangement
offers the advantage that connecting tubing and cannulae can be kept
short, resulting in a very small dead volume and ensuring optimum thermal
stability.
Perfusion
Constant flow perfusion may mimic the in vivo situation more closely,
however, it has the disadvantage that hydrostatic edema becomes
inevitable during vasoconstriction. Constant pressure perfusion permits
higher perfusate flow rates since vasoconstriction decreases perfusate
flow and hydrostatic edema is less likely to occur. The chamber is the
perfusate reservoir (whole blood or erythrocyte containing perfusate).
Perfusion takes place at constant flow. The perfusate is taken from the
reservoir and is passed by means of a peristaltic pump at constant flow
through the deoxygenator and primary heat exchanger, through a second
heat exchanger and a bubble trap to the pulmonary artery and finally into
the lung vascular bed. The venous discharge returns to the reservoir. For
ventilation, a large animal ventilator Model 665 or 663 or a human
ventilator is used.
The chamber also includes our exclusive lung weight measurement system
for continuous monitoring of edema formation.
Extensions
Aerosol nebulizer for inhaled compound challenge Controller for constant
pressure perfusion
Additional Options Include
Transducers and amplifiers for respiratory mechanics
measurement: airflow, tracheal pressure, thoracic
chamber pressure
Transducers and amplifiers for perfusion measure-
ment: realtime flow, perfusion pressure, venous
pressure, temperature etc.
Dedicated software PULMODYN for data acquisi-
tion and analysis of respiratory mechanics and
perfusion
Sensor and amplifier for continuous perfusate
analysis (pO
2
, pH)
Deoxygenation using membrane oxygenator of
whole blood or erythrocyte containing perfusion
Large choice of accessories: ventilators, thermocir-
culator, peristaltic pumps, holders etc...
Benefits and Features
Optimized temperature and humidity conditions for
the isolated lung, unique jacketed thoracic chamber
Continuous measurement of lung weight to
monitor edema formation
Very low flow resistance and minimal dead space
volume virtually eliminate perfusion artifacts
Drug injection pathway built directly into
pulmonary perfusate stream for precision
compound dosing and screening
Removable platform to facilitate surgery
Standard connection for ventilator
More measurement parameters with greater
precision than any other system
Applications
Respiratory mechanics
Airway Flow, Tidal Volume, dynamic
resistance and compliance
Vascular permeability studies
Arterial and venous resistance calculations
Inflammation (?) and Tissue Edema
monitoring
Pharmacology
Compound screening, e.g. action of
histamine, arachinodic acid metabolites
Inhalation toxicology
Environmental toxins, e.g. Ultrafine
particles, ozone, sulphur dioxin...
Biochemistery
Metabolic activity, e.g of biogenic amines,
prostacyclin, angiotensin..
Surfactant bioactivity
Therapy studies
Study of chemotherapy applications using a
human lung lobe after lobectomy of the
lung is performed in lung cancer patient
Order # Product
73-4302 IPL-16 Core System, 115 V
73-4301 IPL-16 Core System, 230 V
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