Gel Filtration Chromatography SpinColumns (Size Exclusion)
QuikPrep® SpinColumns™ for gel fitration chromatography can be used for protein purification, buffer exchange, desalting, or for group separation.
Gel filtration, also called size-exclusion chromatography is an easy-to-use method for separation of molecules with different molecular sizes, using mild conditions.
QuikPrep® SpinColumns™ for gel filtration chromatography can be used for protein purification, buffer exchange, desalting, or for group separation.
Gel filtration, also called size-exclusion chromatography is an easy-to-use method for separation of molecules with different molecular sizes, using mild conditions. Gel Filtration uses the size of molecules in solution to determine separation.
SpinColumns have short media packing so the samples are separated by size, the large molecules travel out of the column with the void volume the smaller molecules, salts, etc. remain in the column. Desalting columns are used not only to remove low molecular weight contaminants such as salt, but also for buffer exchange before and after different chromatography techniques and for the rapid removal of reagents to terminate a reaction.
Examples of group separations include:
- Removal of unincorporated nucleotides during DNA sequencing
- Removal of free low molecular weight labels
- Termination of reactions between macromolecules and low molecular weight reactants
- Removal of products, cofactors or inhibitors from enzymes
- Removal of unreacted radiolabels such as [α-32P]-ATP from nucleic acid labeling reactions
Available Matrices (Packing Materials)
| Matrix | Particle Diameter, µm | Fractionation Range, Da | Exclusion Limit, Da | Applications |
| G-10 | 40 to 120 (dry) | <700 | 700 | Desalting peptides |
| G-25 | 40 to 120 (dry) | 1,000 to 5,000 | 5,000 | Desalting proteins and nucleic acids |
| G-50 | 20 to 80 (dry) | 1,000 to 30,000 | 30,000 | Removal of free labels from labeled macromolecules |
| G-100 | 40 to 120 (dry) | 4,000 to 150,000 | 150,000 | Molecular weight determination |
| P-2 | <45 (wet) | 100 to 1,800 | 1,800 | Rapid carbohydrate and small peptide separations and desalting |
| P-6 | 90 to 180 (wet) |
1,000 to 8,000 | 6,000 | Purification of proteins and polypeptides |
| P-30 | 90 to 180 (wet) |
2,500 to 40,000 | 40,000 | Purification of proteins |
G-10, G-25, G-50- G-100 = Sephadex P-2, P-6, P-30 = Porous polyacrylamide beads
For additional column specifications and technical information, please download our Guide to Gel Fitration or Size Exclusion Chromatography.
Available Matrices (Packing Materials)
| Matrix | Particle Diameter, µm | Fractionation Range, Da | Exclusion Limit, Da | Applications |
| G-10 | 40 to 120 (dry) | <700 | 700 | Desalting peptides |
| G-25 | 40 to 120 (dry) | 1,000 to 5,000 | 5,000 | Desalting proteins and nucleic acids |
| G-50 | 20 to 80 (dry) | 1,000 to 30,000 | 30,000 | Removal of free labels from labeled macromolecules |
| G-100 | 40 to 120 (dry) | 4,000 to 150,000 | 150,000 | Molecular weight determination |
| P-2 | <45 (wet) | 100 to 1,800 | 1,800 | Rapid carbohydrate and small peptide separations and desalting |
| P-6 | 90 to 180 (wet) |
1,000 to 8,000 | 6,000 | Purification of proteins and polypeptides |
| P-30 | 90 to 180 (wet) |
2,500 to 40,000 | 40,000 | Purification of proteins |
G-10, G-25, G-50- G-100 = Sephadex P-2, P-6, P-30 = Porous polyacrylamide beads
SpinColumn Types
| Column Type | Sample Volume | Sample Capacity | Suggested Elution Volume |
Included | Choice of Packing Materials (Matrices) |
| Ultra-Micro | 10 µl to 25 µl | 3 to 30 µg |
28.5 µl |
Two 2 ml centrifuge tubes with top caps |
G-10, G-25, G-50, G-100, P-2, P-6 |
| Micro | 25 µl to 75 µl | 5 to 60 µg |
50 µl |
Two 2 ml centrifuge tubes with top caps | G-10, G-25, G-50, G-100, P-2, P-6 |
| Macro | 75 µl to 150 µl | 30 to 300 µg |
143 µl |
Two 2 ml centrifuge tubes with top and bottom caps | G-10, G-25, G-50, G-100, P-2, P-6, P-30 |
| 96-Well Micro | 25 µl to 75 µl | 5 to 60 µg |
50 µl |
Two 96-well collection plates (1.1 ml per well) | G-10, G-25, G-50, G-100, P-2, P-6 |
| 96-Well Macro | 25 µl to 150 µl |
30 to 300 µg |
143 µl |
Two 96-well collection plates (1.1 ml per well) | G-10, G-25, G-50, G-100, P-2, P-6 |
For additional column specifications and technical information, please download our Guide to Gel Fitration or Size Exclusion Chromatography.