Normal Phase Chromatography SpinColumns
QuikPrep® SpinColumns™ for normal phase chromatography are packed with a stationary polar phase. Available matrixes include: Cyano (CN), Amino (NH2) and PHEA (Polyhydroxyethyl Aspartamide) and Silica. See Description and Specifications for details.
Normal phase chromatography uses a spin column packed with a stationary polar phase, and a non-polar mobile phase is passed through the column. Molecules with similar polarities attract one another causing the least polar compounds to elute first as the more polar compounds are held by the polar matrix. These polar compounds can then be released through the addition of an elution buffer.
QuikPrep® SpinColumns™ for normal phase chromatography are packed with a stationary polar phase. Available matrixes include: Cyano (CN), Amino (NH2) and Hydrophilic PHEA (Polyhydroxyethyl Aspartamide) and Silica.
Available Matrices (Packing Materials)
| Specifications | NH2 | CN | PHEA |
| Particle Size, µm | 25 to 40 | 25 to 40 | 12 |
| Pore Size, À | 60 | 60 | 300 |
| Pore Volume, ml/gm | 0.75 | 0.75 | 0.9 |
| Surface Area, 100 m2/gm | 350 | 350 | 100 |
| % Carbon (w/w) | N/A | N/A | N/A |
| Silica Class | Irregular | Irregular | Type B |
| Acid and Alkali Stable | pH 2 to 8 | pH 2 to 8 |
pH 4 to 6.5 |
CN = Cyano NH2 = Amino PHEA = Polyhydroxyethyl Aspartamide (Hydrophilic)
About Normal Phase Chromatography
Normal phase chromatography uses a column packed with a stationary polar phase, and a non-polar mobile phase is passed through the column. Molecules with similar polarities attract one another causing the least polar compounds to elute first as the polar compounds are held by the polar matrix. These polar compounds can then be released through the addition of an elution buffer
Available Matrices (Packing Materials)
| Specifications | NH2 | CN | PHEA |
| Particle Size, µm | 25 to 40 | 25 to 40 | 12 |
| Pore Size, À | 60 | 60 | 300 |
| Pore Volume, ml/gm | 0.75 | 0.75 | 0.9 |
| Surface Area, 100 m2/gm | 350 | 350 | 100 |
| % Carbon (w/w) | N/A | N/A | N/A |
| Silica Class | Irregular | Irregular | Type B |
| Acid and Alkali Stable | pH 2 to 8 | pH 2 to 8 |
pH 4 to 6.5 |
CN = Cyano NH2 = Amino PHEA = Polyhydroxyethyl Aspartamide (Hydrophilic)
SpinColumn Types
| Column Type | Sample Volume | Sample Capacity | Suggested Elution Volume |
Included | Choice of Packing Materials (Matrices) |
| Ultra-Micro | 10 µl to 25 µl | 3 to 30 µg |
28.5 µl |
Two 2 ml centrifuge tubes with top caps |
Amino, Cyano, Hydrophilic PHEA Silica |
| Micro | 25 µl to 75 µl | 5 to 60 µg |
50 µl |
Two 2 ml centrifuge tubes with top caps | Amino, Cyano, Hydrophilic PHEA Silica |
| Macro | 75 µl to 150 µl | 30 to 300 µg |
143 µl |
Two 2 ml centrifuge tubes with top and bottom caps | Amino, Cyano, Hydrophilic PHEA Silica |
| 96-Well Micro | 25 µl to 75 µl | 5 to 60 µg |
50 µl |
One 96-well collection plate (1.1 ml per well) | Amino, Cyano, Hydrophilic PHEA Silica |
| 96-Well Macro | 25 µl to 150 µl |
30 to 300 µg |
143 µl |
One 96-well collection plates (1.1 ml per well) | Amino, Cyano, Hydrophilic PHEA Silica |