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Standard Vertical Ussing/Diffusion Chamber Systems

The vertical diffusion chamber system is used for transport studies on both filter grown cell monolayers and surgically excised tissue sections. The vertical diffusion chamber system consists of six diffusion chambers, a 12-channel gas manifold and a heat block. 14 general types of vertical chambers are currently available: one for use with cells grown on Snapwell™ membrane supports and the others for use with tissue.
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Item Listing Full Description Specifications Journal Articles Manuals
Item# Description
660003 Standard Vertical Chamber System with Six 66-0014 - 9mm Round Chambers
660037 Standard Vertical Chamber System with Six 66-0036 - 4mm Round Chambers
660002 Standard Vertical Chamber System with Six 66-0013 - 8 x 24mm Oblong Chambers
660033 Standard Vertical Chamber System with Six 66-0032 - 4 x 8mm Oblong Chambers
660039 Standard Vertical Chamber System with Six 66-0038 - 6 x 9mm Oblong Chambers
660047 Standard Vertical Chamber System with Six 66-0046 - 5 x 24mm Oblong Chambers

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Full Description Back to Top

• The standard assay for oral bioavailability
• Applications include:
- Drug delivery
- Toxicology
- Pharmacokinetics
- Metabolic studies
- Transport
• US Patented technology numbers: 5, 183, 760 and 5,591,636
• Available in vertical and horizontal system configurations
• Options for using cultured cells or tissue samples
• Manufactured entirely of acrylic for reduced drug absorption/easy cleaning
• Compact configurations
• Up to 24 chambers can be run simultaneously
• Systems are temperature controlled
• Electrode ports connect optional electrodes for monolayer resistance measurements and electrophysiological determinations • Designed for easy placement of Snapwell™ (grown cells) or tissue sections
• All Snapwell™ and tissue diffusion chamber config-urations are compatible with the vertical system, providing versatility and convenience for correlation studies
• Side-by-side orientation prevents a hydrostatic pressure gradient across cell layer
• Distributes gas (user supplied) to the diffusion chambers where media circulation is generated by gas lift, reducing unstirred fluid layers adjacent to cells
• Individual control valves on gas manifold allow independent adjustment of gas flow into each diffusion chamber half-cell
• Closed perfusion capable

The vertical diffusion chamber system is used for transport studies on both filter grown cell monolayers and surgically excised tissue sections. The vertical diffusion chamber system consists of six diffusion chambers, a 12-channel gas manifold and a heat block. 14 general types of vertical chambers are currently available: one for use with cells grown on Snapwell™ membrane supports and the others for use with tissue.
Gas Manifold
The 12-channel gas manifold distributes gas (user supplied) to the Diffusion Chambers where media circulation is generated by gas lift, reducing unstirred fluid layers adjacent to cells.
Heat Block
The dual heating blocks of the Vertical Chamber System provide efficient and precise heating or cooling of the Diffusion Chamber System. Temperature is controlled by use of a circulating water bath. The Heat Block and Gas Manifold allows six simultaneous tests to be run, improving experimental precision.
Electrode Caps
Electrode caps permit proper positioning of electrodes within the vertical perfusion chambers. They are required for the Snapwell™
BS4 66-0008, 8 x 24 mm Tissue 66-0013 and 9 mm Round
BS4 66-0014 chambers. They are not required for the low volume chambers or the horizontal tissue chambers.
Perfusion Caps
The BS4 66-0019 perfusion cap allow the tissue chambers to be perfused with media using a peristaltic or syringe pump. They are required for use with the Snapwell™ 66-0008, 8 x 24 mm Tissue 66-0013, and 9 mm Round 66-0014 chambers. They are not required for the low volume chambers or the horizontal tissue chambers. We offer 14 different vertical diffusion chambers. We also offer a complete system for each chamber that includes the following components:
Catalog No. Qty. Product
66-0009 1 6-Chamber Heating Block
66-0010 1 Clear Front Panel
66-0048 1 12-Channel Gas Manifold
66-0012 1 Retaining Ring Clamp Tool
Specified 6 Vertical Diffusion Chambers


Specifications Back to Top

Specifications660003660037660002660033660039660047
660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg
Exposed Tissue Area0.64 cm20.12 cm21.78 cm20.29 cm20.46 cm21.15 cm2
Working Reservoir Volume5 to 7 ml5 to 7 ml5 to 7 ml5 to 7 ml5 to 7 ml5 to 7 ml
Chamber Type9 mm Round4 mm Round8 x 24 mm Oblong4 x 8 mm Oblong6 x 9 mm Oblong5 x 24 mm Oblong

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Journal Articles Back to Top

Displaying 11 to 20 of 69results for Standard Vertical Ussing/Diffusion Chamber Systems   Page 1  2  3  4  5  6  7 

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Document

Description

42% Login to view Primary Uroepithelial Cultures.pdf
[971 KB]
Primary Uroepithelial Cultures
42% Login to view macro fast dispo dial.pdf
[241 KB]
Cisplatin delivery from poly(acrylic acid-co-methyl methacrylate) microparticles
33% Login to view electroprep3.pdf
[375 KB]
Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2,and TGF-B 1
30% Login to view PP100 Nonstriatal Dopamine D1 Receptors Regulate Striatal Acetylcholine Release In Vivo.pdf
[215 KB]
Double Syringe Pump use in finding Effects of hyperglycemia
29% Login to view LACK OF RESPONSE SUPPRESSION FOLLOWS REPEATED VENTRAL TEGMENTAL CANNABINOID ADMINISTRATION.pdf
[333 KB]
Lack of response suppression follows repeated ventral tegmental cannabinoid administration: An In Vitro Electrophysiological Study
28% Login to view electroprep1.pdf
[232 KB]
Isolation and purification of two major serum amyloid A isotypes SAA1 and SAA2 from the acute phase plasma of mice
27% Login to view PP06 Molecular Clocks.pdf
[108 KB]
PHD 22/2000 Syringe Pump used in finding Molecular Clocks
26% Login to view PP61 Temporal Gradients in Shear, but Not Spatial Gradients, Stimulate Endothelial Cell Proliferation.pdf
[514 KB]
Temporal Gradients in Shear, but Not Spatial Gradients, Stimulate Endothelial Cell Proliferation
26% Login to view PP15 An Investigation of Three-Phase Flow.pdf
[222 KB]
Syringe Infusion Pump use for An Investigation of Three-Phase Flow
26% Login to view PP114 Microchannel Devices for Efficient Contacting of Liquids in Solvent Extraction.pdf
[62 KB]
Syringe Infusion Pump use in finding Microchannel Devices for Efficient Contacting of Liquids in Solvent Extraction
   

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Manuals Back to Top

Displaying 1 to 1 of 1result for Standard Vertical Ussing/Diffusion Chamber Systems  

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Document

Description

100% Login to view Ussing Chamber Systems.pdf
[1.5 MB]
Guide to Ussing Chamber Systems
   

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