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Standard Vertical Ussing/Diffusion Chamber Systems

The vertical diffusion chamber system is used for transport studies on both filter grown cell monolayers and surgically excised tissue sections. The vertical diffusion chamber system consists of six diffusion chambers, a 12-channel gas manifold and a heat block. 14 general types of vertical chambers are currently available: one for use with cells grown on Snapwell™ membrane supports and the others for use with tissue.
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Item Listing Full Description Specifications Journal Articles Manuals
Item# Description
660003 Standard Vertical Chamber System with Six 66-0014 - 9mm Round Chambers
660037 Standard Vertical Chamber System with Six 66-0036 - 4mm Round Chambers
660002 Standard Vertical Chamber System with Six 66-0013 - 8 x 24mm Oblong Chambers
660033 Standard Vertical Chamber System with Six 66-0032 - 4 x 8mm Oblong Chambers
660039 Standard Vertical Chamber System with Six 66-0038 - 6 x 9mm Oblong Chambers
660047 Standard Vertical Chamber System with Six 66-0046 - 5 x 24mm Oblong Chambers

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Full Description Back to Top

• The standard assay for oral bioavailability
• Applications include:
- Drug delivery
- Toxicology
- Pharmacokinetics
- Metabolic studies
- Transport
• US Patented technology numbers: 5, 183, 760 and 5,591,636
• Available in vertical and horizontal system configurations
• Options for using cultured cells or tissue samples
• Manufactured entirely of acrylic for reduced drug absorption/easy cleaning
• Compact configurations
• Up to 24 chambers can be run simultaneously
• Systems are temperature controlled
• Electrode ports connect optional electrodes for monolayer resistance measurements and electrophysiological determinations • Designed for easy placement of Snapwell™ (grown cells) or tissue sections
• All Snapwell™ and tissue diffusion chamber config-urations are compatible with the vertical system, providing versatility and convenience for correlation studies
• Side-by-side orientation prevents a hydrostatic pressure gradient across cell layer
• Distributes gas (user supplied) to the diffusion chambers where media circulation is generated by gas lift, reducing unstirred fluid layers adjacent to cells
• Individual control valves on gas manifold allow independent adjustment of gas flow into each diffusion chamber half-cell
• Closed perfusion capable

The vertical diffusion chamber system is used for transport studies on both filter grown cell monolayers and surgically excised tissue sections. The vertical diffusion chamber system consists of six diffusion chambers, a 12-channel gas manifold and a heat block. 14 general types of vertical chambers are currently available: one for use with cells grown on Snapwell™ membrane supports and the others for use with tissue.
Gas Manifold
The 12-channel gas manifold distributes gas (user supplied) to the Diffusion Chambers where media circulation is generated by gas lift, reducing unstirred fluid layers adjacent to cells.
Heat Block
The dual heating blocks of the Vertical Chamber System provide efficient and precise heating or cooling of the Diffusion Chamber System. Temperature is controlled by use of a circulating water bath. The Heat Block and Gas Manifold allows six simultaneous tests to be run, improving experimental precision.
Electrode Caps
Electrode caps permit proper positioning of electrodes within the vertical perfusion chambers. They are required for the Snapwell™
BS4 66-0008, 8 x 24 mm Tissue 66-0013 and 9 mm Round
BS4 66-0014 chambers. They are not required for the low volume chambers or the horizontal tissue chambers.
Perfusion Caps
The BS4 66-0019 perfusion cap allow the tissue chambers to be perfused with media using a peristaltic or syringe pump. They are required for use with the Snapwell™ 66-0008, 8 x 24 mm Tissue 66-0013, and 9 mm Round 66-0014 chambers. They are not required for the low volume chambers or the horizontal tissue chambers. We offer 14 different vertical diffusion chambers. We also offer a complete system for each chamber that includes the following components:
Catalog No. Qty. Product
66-0009 1 6-Chamber Heating Block
66-0010 1 Clear Front Panel
66-0048 1 12-Channel Gas Manifold
66-0012 1 Retaining Ring Clamp Tool
Specified 6 Vertical Diffusion Chambers


Specifications Back to Top

Specifications660003660037660002660033660039660047
660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg660013_gr_il_150.jpg
Exposed Tissue Area0.64 cm20.12 cm21.78 cm20.29 cm20.46 cm21.15 cm2
Working Reservoir Volume5 to 7 ml5 to 7 ml5 to 7 ml5 to 7 ml5 to 7 ml5 to 7 ml
Chamber Type9 mm Round4 mm Round8 x 24 mm Oblong4 x 8 mm Oblong6 x 9 mm Oblong5 x 24 mm Oblong

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Journal Articles Back to Top

Displaying 21 to 30 of 69results for Standard Vertical Ussing/Diffusion Chamber Systems   Page 1  2  3  4  5  6  7 

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Document

Description

26% Login to view PP45 Precision Alignment Packaging for Microsystems with Multiple Fluid Connections.pdf
[301 KB]
Union of capillary high-performance liquid chromatography
26% Login to view PP36 Surface Micromachine Microfluidics.pdf
[168 KB]
Differential Involvement of NMDA, AMPA/Kainate, and Dopamine Receptors
26% Login to view PP40IM~1.PDF
[977 KB]
Microdrive Infusion Pump use in finding Impairment in a Discrimination Reversal Task After D1 Receptor Blockade in the Pigeon "Prefrontal Cortex"
25% Login to view HB03 Leukotriene B4 Promotes Reactive Oxidant Generation and Leukocyte Adherence During Acute Hypoxia.pdf
[198 KB]
Homeothermic Blanket Leukotrience B4 promotes reactive oxidant generation and leukocyte adherence during acute hypoxia
23% Login to view PP94 Aquaporin Deletion in Mice Reduces Intraocular Pressure and Aqueous Fluid Production.pdf
[722 KB]
Aquaporin Deletion in Mice Reduces Intraocular Pressure and Aqueous Fluid Production
23% Login to view PP07 Mapping the Glycoprotein Ib-Binding Site in the Von Willebrand Factor A1 Domain.pdf
[312 KB]
Syringe Pump use in Mapping the Glycoprotein Ib-binding Site in the von Willebrand Factor A1 Domain
23% Login to view PP66CH~1.PDF
[208 KB]
Characterization of a novel Sepia officinalis neuropeptide with use of a Syringe Pump
23% Login to view PP67 Muscimol Inactivation of the Dorsal Hippocampus Impairs Contextual Retrieval of Fear Memory.pdf
[296 KB]
Infusion Pump use in finding Muscimol Inactivation of the Dorsal Hippocampus Impairs Contextual Retrieval of Fear Memory
23% Login to view PP108 Effects of Chemotherapy.pdf
[122 KB]
Infusion Pump use in finding Effects of Chemotherapy by 1,3-Bis(2-chloroethyl)-1-nitrosourea
23% Login to view PP92.pdf
[1.4 MB]
Gene transfer of dominant-negative RhoA increases outflow facility in perfused human anterior segment cultures
   

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Manuals Back to Top

Displaying 1 to 1 of 1result for Standard Vertical Ussing/Diffusion Chamber Systems  

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Document

Description

100% Login to view Ussing Chamber Systems.pdf
[1.5 MB]
Guide to Ussing Chamber Systems
   

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