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Home Horizontal Ussing/Diffusion Chamber System Accessories


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Horizontal Ussing/Diffusion Chamber System Accessories

The Horizontal Diffusion Chamber System is designed for transport and toxicology studies using cells and tissues which are exposed to an air interface which occurs in the normal in vivo environment such as with nasal, pulmonary, corneal, or dermal cells. The chambers create an environment in which the apical surface of the tissue or cell monolayer is exposed to liquids, semi-solid compounds or gases while the basolateral surface is perfused with medium.
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Item Listing Full Description Journal Articles Manuals
Item# Description
660017 Heat Block for Horizontal Chambers, for 1 to 6 Chambers
660053 Closed Screws, used when perfusion is not needed, pkg. of 24
660054 Closed Caps for Horizontal Chambers
660055 Open Caps for Horizontal Chambers
660056 Male Luer Fitting - 3/32 Bar, pkg. of 24
660022 Tissue Mounting Ring for Horizontal Chamber Only
660058 Open Screw (straight hole) and O-rings, serves as an electrode holder for Horizontal Diffusion Chambers, pkg. of 24

For local pricing, contact your nearest distributor.



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• Designed for transport studies on cells and tissues which are exposed to an air interface — nasal, corneal, pulmonary or dermal
• Option of using cultured cells or tissue samples makes the horizontal diffusion chamber system versatile and convenient for correlation studies
• Six simultaneous tests can be run, improving experimental precision
• Heat block allows system temperature control with the use of a circulating water bath
• Option of working in open or closed configuration
• Compact configuration 38.1 x 8.9 x 10.2 cm (15 x 3.5 x 4 in) conserves valuable bench space

The Horizontal Diffusion Chamber System is designed for transport and toxicology studies using cells and tissues which are exposed to an air interface which occurs in the normal in vivo environment such as with nasal, pulmonary, corneal, or dermal cells. The chambers create an environment in which the apical surface of the tissue or cell monolayer is exposed to liquids, semi-solid compounds or gases while the basolateral surface is perfused with medium.
The Horizontal Diffusion Chamber System consists of six horizontal chambers, and six closed open chamber tops into which either Snapwell™ devices or tissue mounting rings may be fitted on a horizontal plane, and a heat block. The closed perfusion system also accepts optional diffusion chamber electrodes for resistance measurements and electrophysiology. Either a peristaltic or syringe pump (not included with the system, see Section A in our Full-Line Bioscience Catalog) can be used to perfuse the lower surface of the Snapwell™ insert, usually the basolateral surface. The apical (upper) surface of the diffusion chamber can be used in either the open or closed configuration.
Open Configuration: The upper (apical) surface of the cells is directly accessible, making the system suitable for drug transport or cytotoxicity testing of liquids and semi-solid materials.
Closed Configuration
Cells or tissue can be exposed to solutions, perfused with gas or liquid, or gas pressure can be applied. The closed perfusion system also accepts Diffusion Chamber Electrodes for resistance measurements and electrophysiology.
Diffusion Chamber Electrodes
Diffusion Chamber Electrodes, see diagram on page 191, can be used in both Vertical and Horizontal Diffusion Chambers for making monolayer resistance measurements and electrophysiological determinations. The 2 mm pin connections are compatible with our electrical manifolds that connect the electrodes to various models of Current/Voltage Clamps, see pages M176 to M182. The silver/silver chloride reference electrode consists of a chlorided silver wire contained in a glass barrel that terminates in a ceramic tip. The barrel is filled with any suitable electrolyte solution, usually the buffer used for the external media or 3M KCl, and is refillable. The microporous ceramic tip limits the flux of ions into and out of the electrodes, making them a suitable replacement for bridge systems in most applications. The electrode connects to a wire 1 m in length and is terminated with a 2 mm gold plated pin. We offer a single horizontal diffusion chamber and a 6-chamber system. The system includes the following components:
Catalog No. Qty. Product
66-0017 1 Heat Block for Horizontal Chambers, for 1 to 6 Chambers
66-0016 6 Horizontal Diffusion Chambers


Journal Articles Back to Top

Displaying 11 to 20 of 30results for Horizontal Ussing/Diffusion Chamber System Accessories   Page 1  2  3 

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57% Login to view WR19FA 1.PDF
[220 KB]
Permeation and Activation of the M2 Ion Channel of Influenza A Virus with use of Fast Step Perfusion System
53% Login to view WR04PL 1.PDF
[495 KB]
Two Distinct Ca2+ - Dependent Signaling Pathways regulate the Motor Output of Cochlear Outer Hair Cells with Pneumatic Injection System
47% Login to view Regulate the Motor Output of Cochlear Outer Hair Cells.PDF
[504 KB]
Two Distinct Ca2+ - Dependent Signaling Pathways regulate the Motor Output of Cochlear Outer Hair Cells with Pneumatic Injection System
39% Login to view macro fast dispo dial.pdf
[241 KB]
Cisplatin delivery from poly(acrylic acid-co-methyl methacrylate) microparticles
36% Login to view HS14 Hyperventilation and Cytokine Release.pdf
[422 KB]
Ventilator and Amplifier use in Hyperventilation Inducing release of Cytokines from Perfused Mouse Lung
33% Login to view ET04.pdf
[366 KB]
Energy Integration Describes Sound-Intensity Coding in an Insect Auditory System
31% Login to view flow thru dial.pdf
[387 KB]
A Novel Approach for the Extraction and Concentration of Compounds in Biological Fluids via On-Line Dialysis and Enrichment
31% Login to view Kainate Receptor Activation.pdf
[878 KB]
AMPA Kainate Receptor Activation Mediates Hypoxic Oligodendrocyte Death and Axonal Injury in Cerebral White Matter
31% Login to view electroprep3.pdf
[375 KB]
Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2,and TGF-B 1
30% Login to view HS01 Opioids and Coronary Vascular Tone.pdf
[138 KB]
Force Transducer influence of opioids on the vascular tone of isolated porcine coronary artery segments
   

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Manuals Back to Top

Displaying 1 to 1 of 1result for Horizontal Ussing/Diffusion Chamber System Accessories  

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100% Login to view Ussing Chamber Systems.pdf
[1.5 MB]
Guide to Ussing Chamber Systems
   

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