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Home Horizontal Ussing/Diffusion Chamber System Accessories


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Horizontal Ussing/Diffusion Chamber System Accessories

The Horizontal Diffusion Chamber System is designed for transport and toxicology studies using cells and tissues which are exposed to an air interface which occurs in the normal in vivo environment such as with nasal, pulmonary, corneal, or dermal cells. The chambers create an environment in which the apical surface of the tissue or cell monolayer is exposed to liquids, semi-solid compounds or gases while the basolateral surface is perfused with medium.
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Item Listing Full Description Journal Articles Manuals
Item# Description
660017 Heat Block for Horizontal Chambers, for 1 to 6 Chambers
660053 Closed Screws, used when perfusion is not needed, pkg. of 24
660054 Closed Caps for Horizontal Chambers
660055 Open Caps for Horizontal Chambers
660056 Male Luer Fitting - 3/32 Bar, pkg. of 24
660022 Tissue Mounting Ring for Horizontal Chamber Only
660058 Open Screw (straight hole) and O-rings, serves as an electrode holder for Horizontal Diffusion Chambers, pkg. of 24

For local pricing, contact your nearest distributor.



Full Description Back to Top

• Designed for transport studies on cells and tissues which are exposed to an air interface — nasal, corneal, pulmonary or dermal
• Option of using cultured cells or tissue samples makes the horizontal diffusion chamber system versatile and convenient for correlation studies
• Six simultaneous tests can be run, improving experimental precision
• Heat block allows system temperature control with the use of a circulating water bath
• Option of working in open or closed configuration
• Compact configuration 38.1 x 8.9 x 10.2 cm (15 x 3.5 x 4 in) conserves valuable bench space

The Horizontal Diffusion Chamber System is designed for transport and toxicology studies using cells and tissues which are exposed to an air interface which occurs in the normal in vivo environment such as with nasal, pulmonary, corneal, or dermal cells. The chambers create an environment in which the apical surface of the tissue or cell monolayer is exposed to liquids, semi-solid compounds or gases while the basolateral surface is perfused with medium.
The Horizontal Diffusion Chamber System consists of six horizontal chambers, and six closed open chamber tops into which either Snapwell™ devices or tissue mounting rings may be fitted on a horizontal plane, and a heat block. The closed perfusion system also accepts optional diffusion chamber electrodes for resistance measurements and electrophysiology. Either a peristaltic or syringe pump (not included with the system, see Section A in our Full-Line Bioscience Catalog) can be used to perfuse the lower surface of the Snapwell™ insert, usually the basolateral surface. The apical (upper) surface of the diffusion chamber can be used in either the open or closed configuration.
Open Configuration: The upper (apical) surface of the cells is directly accessible, making the system suitable for drug transport or cytotoxicity testing of liquids and semi-solid materials.
Closed Configuration
Cells or tissue can be exposed to solutions, perfused with gas or liquid, or gas pressure can be applied. The closed perfusion system also accepts Diffusion Chamber Electrodes for resistance measurements and electrophysiology.
Diffusion Chamber Electrodes
Diffusion Chamber Electrodes, see diagram on page 191, can be used in both Vertical and Horizontal Diffusion Chambers for making monolayer resistance measurements and electrophysiological determinations. The 2 mm pin connections are compatible with our electrical manifolds that connect the electrodes to various models of Current/Voltage Clamps, see pages M176 to M182. The silver/silver chloride reference electrode consists of a chlorided silver wire contained in a glass barrel that terminates in a ceramic tip. The barrel is filled with any suitable electrolyte solution, usually the buffer used for the external media or 3M KCl, and is refillable. The microporous ceramic tip limits the flux of ions into and out of the electrodes, making them a suitable replacement for bridge systems in most applications. The electrode connects to a wire 1 m in length and is terminated with a 2 mm gold plated pin. We offer a single horizontal diffusion chamber and a 6-chamber system. The system includes the following components:
Catalog No. Qty. Product
66-0017 1 Heat Block for Horizontal Chambers, for 1 to 6 Chambers
66-0016 6 Horizontal Diffusion Chambers


Journal Articles Back to Top

Displaying 21 to 30 of 30results for Horizontal Ussing/Diffusion Chamber System Accessories   Page 1  2  3 

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Description

28% Login to view LACK OF RESPONSE SUPPRESSION FOLLOWS REPEATED VENTRAL TEGMENTAL CANNABINOID ADMINISTRATION.pdf
[333 KB]
Lack of response suppression follows repeated ventral tegmental cannabinoid administration: An In Vitro Electrophysiological Study
28% Login to view MC01 Impaired Stratum Corneum Hydration in Mice Lacking Epidermal Water Channel Aquaporin-3.pdf
[339 KB]
Metabolic Cages use in finding Impaired Stratum Corneum Hydration in Mice Lacking Epidermal Water Channel Aquaporin-3
27% Login to view PP33PR 1.PDF
[393 KB]
Micro/nano spacecraft thermal control using a MEMS-based pumped liquid cooling system with use of Programmable syringe pump
23% Login to view Action of 2,3-butanedione monoxime on capacitance.PDF
[352 KB]
Pneumatic Injection System use in finding Action of 2,3-butanedione monoxime on capacitance and electromotility of guinea-pig cochlear outer hair cells
23% Login to view WR03PL 1.PDF
[344 KB]
Pneumatic Injection System use in finding Action of 2,3-butanedione monoxime on capacitance and electromotility of guinea-pig cochlear outer hair cells
21% Login to view HS09TY Sarcoplasmic Reticulum Ca2+ Uptake and release.pdf
[1.3 MB]
Sarcoplasmic Reticulim Ca2+ Uptake and Release
18% Login to view HS03F3 Potassium channal inhibition in coronary arteries.pdf
[536 KB]
Force Displacemen Transducer, 5-hydroxytryptamine is a mediator of 4-aminopyridine induced contractions in porcine and human isolated coronary arteries
15% Login to view HS15 Myoglobin facilitates oxygen diffusion.pdf
[631 KB]
Isolated Heart Apparatus use in Myoglobin facilitating oxygen diffusion
15% Login to view HS19 Soluble Guanylyl Cyclase in Aging and Hypertension.pdf
[238 KB]
hy010000043p
15% Login to view HS02IS Evaluation of Skeletonized LITA.pdf
[228 KB]
Isolated Organ Set use in Intraoperative and Laboratory Evaluation of Skeletonized Versus Pedicled Internal Thoracic Artery
   

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Manuals Back to Top

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100% Login to view Ussing Chamber Systems.pdf
[1.5 MB]
Guide to Ussing Chamber Systems
   

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