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Harvard Apparatus
 
 
 Pump Selection Guide 
 
Choosing the Right Pump for Your Application & Budget
    Pump Types: Advantages & Disadvantages
    Syringe Pump Selection Guide
      Infusion Only Syringe Pumps
      Infusion/Withdrawal Syringe Pumps
      Push/Pull Syringe Pumps
    Syringe Pump Application Guide
    Peristaltic Pump Selection Guide
How to Calculate the Pressure Requirement of Your Experiment
Syringe Pump Pressure & Flow Rate
    Minimum/Maximum Flow Rates by Pump & Syringe Size
      Pump 11 Pico Plus Flow Rates
      PHD 22/2000 Hpsi Flow Rates
      Pump 11 Plus Flow Rates
      Pump 22 Flow Rates
      PHD ULTRA™ Flow Rates
      PHD 22/2000 and PHD 4400 Hpsi Flow Rates
Common Syringe Data
    How to Select the Correct Syringe for Your Application
      Syringe Specifications
      Pressure Unit Conversion Chart
      Force Conversion Table
 

Harvard Apparatus offers a broad selection of syringe, peristaltic and continuous flow pumps to suit almost
every application.

Syringe pump models have been expanded to include new innovative pumps with the widest range of flow rates and forces of any manufacturer. The following guide and tables were designed to answer most questions regarding syringe and peristaltic pumps. Please review the information then contact our technical support department for further assistance, if needed.

Harvard Apparatus has a long history of inventing, innovating and manufacturing syringe pumps. Harvard Apparatus invented the lead screw based syringe pump in the 1950’s and introduced the first microprocessor pump, the now legendary Pump 22, in the 1980’s. Our syringe pumps are so accurate, even at low flow rates, that they have become the standard for mass spectrometry calibration and anywhere accurate volumes must be delivered.

The innovations continued with the additions of the PHD 22/2000 Pumps with enormous pressure and flow capability; the 11 Plus Pumps, the standard for general laboratory experiments; the Pump 11 Pico Plus Pump for picoliter and small volume injections; an entire selection of peristaltic pumps; an expanded line of component pumping modules for the OEM and do-it-yourself markets, and much, much more.

Our newest pumps, The New PHD Ultra™ Pumps have a GUI Touchscreen and advanced programming capabilities for creating simple to complex methods. These user friendly pumps produce extremely smooth and accurate flows at a wide flow rate range.

 

 
 
Choosing the Right Pump
for Your Application & Budget
| TOP |

We are frequently asked to assist in the selection of the appropriate pump for a variety
of research applications. The following list was developed as a guide to help you quickly and easily choose the right pump for your application. Consider the Syringe Pump Questions or the Peristaltic Pump Questions listed in this guide when selecting your pump. If your specifications do not appear to be met by these pumps, please call our technical support department for further assistance.

  1. Brief overview of advantages and disadvantages of each type of pump.
  2. Syringe Pump Selection Guide.
  3. Syringe Pump Application Guide.
  4. Peristaltic Pump Selection Guide.
 
Pump Types: Advantages & Disadvantages 
Syringe Pumps

(PHD ULTRA™ Programmable and
Pump 11 Pico Plus shown)

Syringe pumps provide the most accurate
delivery of fluids. They use a syringe for the
fluid reservoir. The syringe pump motor moves the pusher block forward which depresses the syringe plunger causing the dispensing of fluid.

A motor driven (lead screw) slowly turns, moving the plunger of the syringe in, and pushing the fluid out (infuse). Reversing the direction of the motor moves the plunger back allowing you to fill the syringe (withdraw).

wAdvantages

  • Works at pressures up to 3000 psi
  • Highest precision < 0.1%
  • Pulse free flow
  • Accurately dispense very small to
    large volumes < ±.35%
  • Easily sterilizable
  • Can dispense or withdraw
  • Many easily programmable
    dispensing profiles: gradients
  • 1 to 10 channels of operation

wDisadvantages

  • Pumps finite volumes
  • Slightly more expensive

wTypical Applications

  • Pumping sample/calibrant into
    Mass Spectrometer
  • Accurate dispensing of drugs in animals
  • High pressure flow into
    reaction chamber
 
Peristaltic Pumps

(MPII Mini-Peristaltic and
66/77 Peristaltic Pumps Shown)

Peristaltic pumps dispense fluid using a rotating head mechanism. The rotating head has a number of rollers that depress the tubing driving the fluid forward. These pumps have an external reservoir and therefore can accommodate a much larger volume of fluid.

In this example three rollers on rotating arms pinch the tube against an arc and push the fluid along. There are usually three or four sets of rollers.

wAdvantages

  • Pumps continuous volumes
  • Sterilizable
  • Less expensive for multiple
    channel dispensing
  • 1 to 16 channels of operation
  • Can dispense or withdraw

wDisadvantages

  • Low pressure operation, 30 psi or less
  • Pulsing flow
  • Moderate precision 1 to 3%

wTypical Applications

  • Perfusion flow across tissue or cells
  • Pump in and out with balanced flow
  • Transfer bulk liquids e.g., controlled
    animal feeding
 
Syringe Pump Selection Guide 
 

 Syringe Pump Questions

1. What is your application?

2. Does the pump need to withdraw (fill the syringe) as well as infuse (dispense)?

3. What is the total volume to be delivered?

4. What size syringe(s) will be used?

5. What flow rate(s) are you working with?

6. How many syringes will be used simultaneously?

7. What is the viscosity of the liquid you are pumping?
7. See Pump Reference.

8. What are the pressure requirements of your experiment?
8. See Syringe Pump Pressure and Flow Rate.

9. Does the pump need to continuously infuse over a
2. 24 hour period of time?

10. Does the pump need to be programmable?
10. (Create and run up to 50 methods).

11. Does the pump need to be controlled with a computer?

12. Does the pump need to have TTL capabilities
12. (ex. external control of valves, use of footswitch, etc)?

 
Features & Specifications 
WInfusion Only Syringe Pumps
WPump Model Pump 11 Plus Pump 11 Plus Pump 22 Physio 22 PHD ULTRA™ PHD 22/2000
WStandard
WOrder #
70-2208 70-2209 55-2222 70-2222 70-3005 70-2000
WProgrammable
WOrder #
Number of Syringes 1 2 2 to 10* 2 to 10* 2 to 10* 2 to 10*
Minimum
Syringe Size
0.5 µl 0.5 µl 0.5 µl 0.5 µl 0.5 µl 0.5 µl
Maximum
Syringe Size
50/60 ml 10 ml 140 ml 140 ml 140 ml 140 ml
Minimum Flow Rate 0.0014 µl/hr 0.0014 µl/hr 0.002 µl/hr 0.002 µl/hr 1.56 pl/min 0.0001 µl/hr
Maximum Flow Rate 26.56 ml/min 7.91 ml/min 55.1 ml/min 55.1 ml/min 220.97 ml/min 220.82 ml/min
Average Linear
Force (lbs)
16 16 47 47 75 (Adjustable) 50 or 66**
RS-232
Computer Control
No No Yes Yes Yes Yes
USB
Computer Control
No No No No Yes No
TTL Connection No No Yes Yes Yes Yes
Control Box
Dimensions
(H x W x D)
13 x 22.9 x 11.4 cm
(5 x 9 x 4.5 in)
13 x 22.9 x 11.4 cm
(5 x 9 x 4.5 in)
28 x 22.2 x 14 cm
(11 x 8.75 x 5.5 in)
28 x 22.2 x 14 cm
(11 x 8.75 x 5.5 in)
18.4 x 21.6 x 30.5 cm
(7.25 x 8.5 x 12 in)
15.9 x 22.8 x 27.9 cm
(6.3 x 9 x 11 in)
Pump Head Dimensions
(H x W x D)
Weight 2.1 kg (4.6 lbs) 2.1 kg (4.6 lbs) 4.5 kg (10 lbs) 4.5 kg (10 lbs) 4.5 kg (10 lbs) 4.5 kg (10 lbs)
* Depends upon the Syringe Rack
** Available in Standard Force= 50 lbs or High Force= 66 lbs.
 
W Infusion/Withdrawal Syringe Pumps
WPump Model Pump 11 Plus Pump 11 Plus Pico Plus Nanomite Pump 22 PHD ULTRA™
WStandard
WOrder #
70-2211 70-2212 70-2213 70-2217 55-2226 70-3006
WProgrammable
WOrder #
70-3007
Number of
Syringes
1 2 2 1 2 to 10* 2 to 10*
Minimum
Syringe Size
0.5 µl 0.5 µl 0.5 µl 0.5 µl 0.5 µl 0.5 µl
Maximum
Syringe Size
50/60 ml 10 ml 10 ml 1 ml 140 ml 140 ml
Minimum Flow Rate 0.0014 µl/hr 0.0014 µl/hr 1.3 pl/min 3.3 nl/min 0.002 µl/hr 1.56 pl/min
Maximum Flow Rate 26.56 ml/min 7.91 ml/min

0.4394 ml/min

1901 µl/min 55.1 ml/min 220.97 ml/min
Average Linear
Force (
lbs)
16 16 25 12 47 75 (Adjustable)
RS-232
Computer Control
Yes Yes Yes Yes Yes Yes
USB
Computer Control
No No No No No Yes
TTL Connection No No Footswitch Footswitch Yes Yes
Control Box
Dimensions
(H x W x D)
13 x 22.9 x 11.4 cm
(5 x 9 x 4.5 in)
13 x 22.9 x 11.4 cm
(5 x 9 x 4.5 in)
11.4 x 22.9 x 11.4 cm
(4.5 x 9 x 4.5 in)
8.9 x 22.9 x 11.4 cm
(3.5 x 9 x 4.5 in)
28 x 22.2 x 14 cm
(11 x 8.75 x 5.5 in)
18.4 x 21.6 x 30.5 cm
(7.25 x 8.5 x 12.0 in)
Pump Head
Dimensions
(H x W x D)
1 x 18.5 x 5 cm
(2.5 x 7.25 x 2 in)
 
Weight 2.1 kg (4.6 lbs) 2.1 kg (4.6 lbs) 2.3 kg (5 lbs) 2.06 kg (4.6 lbs) 4.5 kg (10 lbs) 4.5 kg (10 lbs)
* Depends upon the Syringe Rack
** Available in Standard Force= 50 lbs or High Force= 66 lbs.
 
WInfusion/Withdrawal Syringe Pumps (continued)
WPump Model PHD
22/2000
MRI PHD
22/2000
PHD
22/2000 Hpsi
PHD
ULTRA™ Hpsi
PHD
4400 Hpsi
PHD ULTRA™
4400 Hpsi
WStandard
WOrder #
70-2001 70-2130
WProgrammable
WOrder #
70-2002 70-2131 70-2023 70-3111 70-2200 70-3010
Number of Syringes 2 to 10* 2 4 4 1 2
Minimum
Syringe Size
0.5 µl 0.5 µl 20 ml 20 ml 2.5 ml 0.5 µl
Maximum
Syringe Size
140 ml 140 ml 200 ml 200 ml 100 ml 140 ml
Minimum Flow Rate 0.0001 µl/hr 0.0001 µl/hr 1.5 µl/hr 1.5 µl/hr 0.0076 µl/min 0.0004 µl/hr
Maximum Flow Rate 220.82 ml/min 220.82 ml/min

112 ml/min

112µl/min 182.40 ml/min

106.6 ml/min

Average Linear
Force (lbs)
50 or 66** 50 433 433 200 200
RS-232
Computer Control
Yes Yes Yes Yes Yes Yes
USB
Computer Control
No No No Yes No Yes
TTL Connection Yes Yes Yes Yes Yes Yes
Control Box Dimensions
(H x W x D)
15.9 x 22.8 x 27.9 cm
(6.3 x 9 x 11 in)
9.5 x 27.9 x 22.9 cm
(3.75 x 11 x 9 in)
9.5 x 27.9 x 22.9 cm (3.75 x 11 x 9 in) 9.5 x 27.9 x 22.9 cm (3.75 x 11 x 9 in)
Pump Head Dimensions
(H x W x D)
22.9 x 43.2 x 30.5 cm
(9 x 17 x 12 in)
22.9 x 43.2 x 30.5 cm
(9 x 17 x 12 in)
22.9 x 43.2 x 30.5 cm
(9 x 17 x 12 in)
Weight 4.5 kg (10 lbs) 13.6 kg (30 lbs) 18.18 kg (40 lbs) 18.18 kg (40 lbs) 6.4 kg (14 lbs) 6.4 kg (14 lbs)
* Depends upon the Syringe Rack
** Available in Standard Force= 50 lbs or High Force= 66 lbs.
 
W Push/Pull Syringe Pumps
WPump Model PHD 22/2000 PHD ULTRA™
WStandard
WOrder #
70-2020 or 71-2020 70-3008
WProgrammable
WOrder #
70-2019 or 71-2019 70-3009
Programmable
Pump Model
Available Available
Number of Syringes 4 (2 on each side
of pusher block)
4 (2 on each side
of pusher block)
Minimum Syringe Size 0.5 µl 0.5 µl
Maximum Syringe Size 140 ml+ 140 ml+
Minimum Flow Rate 0.0001µl/hr 1.56 pl/hr
Maximum Flow Rate 70.518 ml/min 220.97 ml/min
Average Linear
Force (lbs)
50 or 60** 75 (Adjustable)
RS-232 Computer Control Yes Yes
USB Computer Control No Yes
TTL Connection Yes Yes
Control Box Dimensions
(H x W x D)
15.9 x 22.8 x 27.9 cm
(6.3 x 9 x 11 in)
18.4 x 21.6 x 30.5 cm
(7.25 x 8.5 x 12.0 in)
Pump Head Dimensions
(H x W x D)
Weight 4.5 kg (10 lbs) 4.5 kg (10 lbs)
 
Syringe Pump Application Guide 
Accurate Delivery of Coatings                  
Animal Feeding                
Biomedical Dispensers                    
Bulk Fluid Transfer                      
Cell Cultures                      
Cellular Injection              
Continuous Infusions                      
Doping                

Drug Delivery (same infusion rates)

               

Drug Delivery
(different infusion rates)


(RS232)*
         
(RS232)*

(USB)*
   
Drug Delivery (time released)                
Drug Development                    
Electrospinning                
Fluid Blending
(RS232)*
     
(RS232)*
     

Fluid Blending
(2 independent channels)

                     
Fluid Sampling            
Gradients
(RS232)*
   
(RS232)*
 
(RS232)*
or USB*
*

(RS232)*
         
High Pressure Injection            
Highly Corrosive Fluids              
HPLC                

Injecting Into High
Pressure Reaction Vessels

           
Injection Pressure Calculations                
Instrument Injections                
Low Pressure Chromatography                  
Mass Spectometry              
Medical Coating Delivery                      
Microdialysis              
MRI Studies                    

Multiple Simultaneous
Feeding Stations


(RS232)*
or USB*
*
     

(RS232)*

(RS232)*
       
Nano Fluidics                  
Nutritional Studies              
OEM Modules                        
Oocyte Applications                  
Patch Clamping                    

Remote Pumping
of Hazardous Material

         
Stereotaxic Devices                    
Titrations
(RS232)*
   
(RS232)*
             
Viscous Solutions            
* Can be done using RS232 or USB computer control.
Peristaltic Pump Selection Guide 
 
  • Broad selection of pumps for every application
  • Wide range of flow rates ml/hr to L/min
  • Multiple multi-channel models with up to 32 channels
  • Peristaltic and non-peristaltic pumps
  • Continuous delivery and batch mode dispensing

Harvard Apparatus now offers an extensive selection of peristaltic and other continuous flow pumps to suit the needs of a wide range of research applications. Pumps which offer features such as multi-channel pumping, computer control, analog control, low electrical noise and a wide range of fluid flow rates are now available. Pump styles include traditional roller type peristaltic pumps, our exclusive double linear sinusoidal peristaltic pump, shuttle pumps which utilize check valves, and diaphragm pumps. The following table was designed to answer most questions regarding our continuous flow pumps. Please contact our technical support department for further assistance.

Peristaltic and Continuous Flow Pumps

Traditional peristaltic pumps utilize a series of rollers (1 to 8) to push fluid through tubing held within a pump head. Peristaltic flow is typically pulsatile, but can be made smoother with the use of more rollers in the pumping head. Our Mini-Peristaltic Pump (MPII) features two speed ranges,reversability and dual channel pumping at a very reasonable price. Many pumps offer external control either through the input of an analog signal proportional to the speed or by RS-232 (serial) communication.

 Peristaltic Pump Questions

1. How many channels (tubes) will be used simultaneously?

2. What size tubing will be used (inner diameter)?

3. What flow rate(s) will be used?

4. What is the total volume to be delivered?

5. Do you need continuous flow?

6. Does the pump need to be battery operated?

7. Do you need to control the pump with a computer?

8. Do you need analog control?

9. Does the pump need to have TTL capabilities
> (external control of valves, use of footswitch etc)?

 
WPeristaltic Pump Selection Guide
WPump Model MPII Pump 66 Pump 77 Model 720 Model 720 Model 720 Shuttle Pump
WOrder # 70-2027 55-7766 55-7777 72-0002 61-0098 72-0001 61-0128,
72-0011
Number of Channels 1 to 2 1 1 1 to 2 1 to 2 1 to 2 2
Number of Rollers 4 3 3 3 3 3 1
Tube Size
(Inner Diameter)
1.6 mm (1/16 in) 1.6 mm and 3.2 mm
(1/16 in and 1/8 in)
3.2 mm and 6.4 mm
(1/8 in and 1/4 in)

0.38 mm to 2.4 mm
(0.015 in to 0.093 in)

0.38 mm to 2.4 mm
(0.015 in to 0.093 in)
0.38 mm to 2.4 mm
(0.015 in to 0.093 in)
Flow Rate (ml/min/Per Channel)
MMinimum 0.8 0.01 0.01 0.00033 0.0033 0.033 1.25
MMaximum 12.25 210 750 0.2 2.42 18.3 25
RS-232
Computer Control
No Yes Yes No No No No
TTL Control No Yes Yes No No No No
Analog Control No No No Yes Yes Yes No
Power 115/230 VAC
50/60 Hz
115/230 VAC
50/60 Hz
115/230 VAC
50/60 Hz
120 VAC, 50 Hz
230 VAC, 60 Hz
120 VAC, 50 Hz
230 VAC, 60 Hz
120 VAC, 50 Hz
230 VAC, 60 Hz
120 VAC, 50 Hz
230 VAC, 60 Hz
Battery Backup No No No 9V Lithium Battery
up to 30 hours
9V Lithium Battery
up to to 30 hours
No 9V Lithium Battery
up to 50 hours
Dimensions
(H x W x D)
189 x 114 x 105 cm
(3.5 x 4.5 x 4 in)
22.9 x 20.6 x 8.9 cm
(9 x 8.125 x 3.5 in)
24.1 x 20.6 x 12.7 cm
(9.5 x 8.125 x 5 in)
6.4 x 5.7 x 10.2 cm
(2.5 x 2.25 x 4 in)
6.4 x 5.7 x 10.2 cm
(2.5 x 2.25 x 4 in)
6.4 x 5.7 x 10.2 cm
(2.5 x 2.25 x 4 in)
3.3 x 11.4 x 7.2 cm
(1.3 x 4.5 x 2.8 in)
Weight 0.96 kg (2.1 lbs) 3.53 kg (7.85 lbs) 5.1 kg (11.25 lbs) 375 g 375 g 375 g
 
WPeristaltic Pump Selection Guide (continued)
WPump Model Ecoline
(VC Series)
REGLO Digital REGLO Analog Microprocessor
Controlled
Ecoline
(VC-MS Series)
WOrder #
Number of Channels 1 2 or 4 2 or 4 4, 8, 12, 16 or 24 4 or 8
Number of Rollers 2 or 3 6, 8 or 12 6, 8 or 12 8 6 or 12
Tube Size
(Inner Diameter)
Various Various Various

Various

Various
Flow Rate (ml/min/Per Channel)
MMinimum Depends Upon Model Depends Upon Model Depends Upon Model Depends Upon Model Depends Upon Model
MMaximum Depends Upon Model Depends Upon Model Depends Upon Model Depends Upon Model Depends Upon Model
RS-232
Computer Control
No Yes No Yes* No
TTL Control No Yes Yes Yes* No
Analog Control Yes No Yes Yes* Yes
Power 115/230 VAC
50/60 Hz
115/230 VAC
50/60 Hz
115/230 VAC
50/60 Hz
115/230 VAC
50/60 Hz
115/230 VAC
50/60 Hz
Battery Backup No No No No No
Dimensions
(H x W x D)
13.8 x 16.9 x 25.6 cm
(5.4 x 6.7 x 10.1 in)
13.5 x 10.0 x 19.0 cm
(5.3 x 3.9 x 7.5 in)
Dimension for
4-channel pump
14.3 x 10.0 x 19.0 cm
(5.6 x 3.9 x 7.6 in)
Dimension for
4-channel pump
13.8 x 16.9 x 31.3 cm
(5.4 x 6.7 x 12.3 in)
Dimension for
8-channel pump
Weight 5.3 kg (11.7 lbs) 2.1 kg (4.6 lbs) 2.1 kg (4.6 lbs) 5.5 kg (12.1 lbs)
* IP Without Dispensing has Analog interface Only
 
 
How to Calculate the Pressure Requirement
of Your Experiment
| TOP |

The following chart will help you determine the pressure requirement of your experiment. This is important in selecting the correct pump with the proper psi capability for your application. Choose the selections that are the closest to your experimental conditions or write in your actual values. Once you have filled in the chart call us for technical assistance if needed.           

  1. Nature of the sample you are flowing into (Application)
  2. The flow rate of the material
  3. The surface area of the syringe and the linear force capability
    on screen.
    Capability of the pump
  4. The tubing diameter
  5. The tubing length
  6. Viscosity of the material being pumped
  7. The temperature of the material being pumped
 
 
 
Syringe Pump Pressure & Flow Rate
| TOP |
How to Calculate the Pressure of Various Syringe Sizes
 

The pressure that a syringe pump can generate is a function of both the force of the pump (measured at the pusher block in pounds) as well as the physical characteristics of the syringe and setup used. The following table compares various syringe pumps and the pressures in PSI (pounds per square inch). Each data point was calculated by dividing the average pump force by the surface area (in square inches) of syringes with diameters from 0.1 to 50 mm. Diameters and surface areas for a variety of syringes can be found in the table on page 13. This table is intended to be a guide of total pressures generated.  Actual values may be higher or lower than the listed pressures due to the influence of other factors such as tubing diameter and length.  When using more than one syringe sharing the same pusher block, the pressure is calculated by dividing the force (lbs) by the total surface area (square inches) of all syringes on the pump. For example, nominal pressure obtained using two 25 ml Hamilton Gastight® syringes on a PHD 22/2000 standard pressure syringe pump would be: 50 lbs / (0.644 in2 X 2) = 38.81 PSI (2.68 bars).

 
WPump Average PressureA (PSI)B
Syringe Size Syringe Diameter (mm) Pump 11 Plus Pump 11 Pico Plus Pump 22 PHD 22/2000 PHD 22/2000 Hpsi PHD ULTRA™ PHD 4400 Hpsi
0.5 µl 0.1 >1000 >1000 >1000 >1000 >1000 >1000
10 µl 0.5 >1000 >1000 >1000 >1000 >1000 >1000
50 µl 1 >1000 >1000 >1000 >1000 >1000 >1000
1 ml 5 526 821 >1000 >1000 >1000 >1000
5 ml 10 131 205 386 394 616 1438
10 ml 15 58 91 172 175 273 639
50 ml 25 21 33 62 63 569 98 230
Force (lbs)   16 25C 47 48 433 75 200

A. Calculated pressure based on pump force at average speed

  • Higher pressures may be achieved at minimum speed and lower pressures at maximum speed.
  • Pump speed and force are inversely proportional.
  • Most syringes are pressure rated and may not be able to tolerate pressure generated by the syringe pump.
    Consult harvard Apparatus on your syringe manufactorer for syringe details and specifications.

B. To convert pressure from PSI to bars use the following equation: bar pressure = PSI x 0.0690

C. Actual force is higher: Use of pump with greater back pressure may result in premature wear of syringe pump halfnut.

 

Minimum/Maximum Flow Rates By Pump and Syringe Size 

 

Flow rates were calculated based on the pusher block travel rate for each pump (rate at which the syringe pump moves the syringe plunger) and the diameter of the syringe.

 
 Pump 11 Pico Plus Flow Rates
Syringe
Size
Nominal
Diameter,
mm*
Minimum Maximum
0.5 µl 0.10 1.3 pl/min 20.00 nl/min
1 µl 0.15 3.0 pl/min 46.00 nl/min
10 µl 0.46 27.0 pl/min 400.00 nl/min
100 µl 1.46 270.0 pl/min 0.004 ml/min
1000 µl 4.61 2,690.0 pl/min 0.043 ml/min
10 ml 14.57 27.0 nl/min 0.439 ml/min
* Note: These figures have been rounded and therefore may not exactly match the Syringe Diameter Chart.
 
PHD 22/2000 Hpsi Flow Rates*
(High Volume and Pressure)
Syringe
Size
Diameter,
mm
Minimum Maximum
20 ml 19.13 1.5 µl/hr 20 ml/min
50 ml 28.60 3.4 µl/hr 46 ml/min
100 ml 34.90 5.0 µl/hr 68 ml/min
200 ml 44.75 8.2 µl/hr 112 ml/min
* Note: The rates listed are for single stainless steel syringe
 
W Pump 11 Plus Flow Rates
Syringe Size Diameter,
mm*
Minimum Maximum
0.5 µl 0.10 0.0014 µl/hr 0.0003 µl/min
1 µl 0.15 0.0031µl/hr 0.0008 µl/min
2 µl 0.21 0.0061 µl/hr 0.0016 µl/min
5 µl 0.33 0.0149 µl/hr 0.0040 µl/min
10 µl 0.46 0.0289 µl/hr 0.0078 µl/min
25 µl 0.73 0.0728 µl/hr 0.0198 µl/min
50 µl 1.03 0.1448 µl/hr 0.0395 µl/min
100 µl 1.46 0.2909 µl/hr 0.0794 µl/min
250 µl 2.30 0.7218µl/hr 0.1970 µl/min
1000 µl 4.61 1.451 µl/hr 0.3959 µl/min
1 ml 5.00 2.900 µl/hr 0.7918 µl/min
2.5 ml 7.28 to 9.6 7.232 µl/hr 1.974 µl/min
3 ml 8.66 to 9.0 10.24 µl/hr 2.794 µl/min
5 ml 10.3 to 13.0 14.50 µl/hr 3.952 µl/min
10 ml 14.57 to 15.9 28.97 µl/hr 7.909 µl/min
20 ml 19.13 to 20.05 54.86 µl/hr 14.97 µl/min
30 ml 21.7 to 23.2 72.81 µl/hr 19.88 µl/min
50 ml 26.7 to 32.6 97.27 µl/hr 26.56 µl/min
* Note: These figures have been rounded and therefore may not exactly match the
Syringe Diameter Chart.
 
W Pump 22 Plus Flow Rates
Syringe Size Diameter,
mm*
Minimum Maximum
0.5 µl 0.10 0.002 µl/hr 23.8 µl/hr
1 µl 0.15 0.003 µl/hr 47.8 µl/hr
2 µl 0.21 0.006 µl/hr 95.2 µl/hr
5 µl 0.33 0.015 µl/hr 238.0 µl/hr
10 µl 0.46 0.029 µl/hr 474.0 µl/hr
25 µl 0.73 0.073 µl/hr 1193.0 µl/hr
50 µl 1.03 0.002 µl/min 39.7 µl/min
100 µl 1.46 0.005 µl/min 79.7 µl/min
250 µl 2.30 0.012 µl/min 197.8 µl/min
500 µl 3.26 0.024 µl/min 397.0 µl/min
1000 µl 4.61 0.048 µl/min 795.0 µl/min
1 ml 5.00 0.049 µl/min 805.0 µl/min
2 ml 9.00 0.011 ml/hr 186.6 ml/hr
2.5 ml 7.28 to 9.6 0.10 ml/hr 168.2 ml/hr
3 ml 8.66 to 9.0 0.011 ml/hr 181.4 ml/hr
5 ml 10.3 to 13.0 0.019 ml/hr 317.0 ml/hr
10 ml 14.57 to 15.9 0.028 ml/hr 461.0 ml/hr
20 ml 19.13 to 20.05 0.050 ml/hr 821.0 ml/hr
30 ml 21.7 to 23.2 0.074 ml/hr 1208.8 ml/hr
50 ml 26.7 to 32.6 0.002 ml/min 28.4 ml/min
100 ml 34.9 to 35.7 0.003 ml/min 47.6 ml/min
140 ml 38.40 0.004 ml/min 55.1 ml/min
* Note: These figures have been rounded and therefore may not exactly match the Syringe Diameter Chart.
 
W PHD ULTRA™ Flow Rates
Syringe Size Diameter,
mm*
Minimum Maximum
0.5 µl 0.103 1.500 pl/min 1.589 µl/min
1 µl 0.146 3.000 pl/min 3.180 µl/min
2 µl 0.206 6.000 pl/min 6.358 µl/min
5 µl 0.343 16.620 pl/min 17.630 µl/min
10 µl 0.485 33.300 pl/min 35.249 µl/min
25 µl 0.729 75.240 pl/min 79.640 µl/min
50 µl 1.03 150.300 pl/min 158.984 µl/min
100 µl 1.457 300.720 pl/min 318.126 µl/min
250 µl 2.304 752.100 pl/min 795.51 µl/min
500 µl 3.256 1.502 nl/min 1.588 ml/min
1000 µl 4.608 3.008 nl/min 3.181 ml/min
1 ml 4.699 3.128 nl/min 3.308 ml/min
3 ml 8.585 10.442 nl/min 11.044 ml/min
5 ml 11.989 20.365 nl/min 21.539 ml/min
10 ml 14.427 29.490 nl/min 31.190 ml/min
20 ml 19.05 51.418 nl/min 54.383 ml/min
30 ml 21.59 66.043 nl/min 69.852 ml/min
50 ml 26.594 100.205 nl/min 105.985 ml/min
100 ml 35.7 180.575 nl/min 190.992 ml/min
140 ml 38.4 208.922 nl/min 220.974 ml/min
 
 
 
W PHD 22/2000 and PHD 4400 Flow Rates
Syringe Size Diameter,
mm*
Minimum Maximum
0.5 µl 0.10 0.0001 µl/hr 95.330 µl/hr
1 µl 0.15 0.0002 µl/hr 190.70 µl/hr
2 µl 0.21 0.0004 µl/hr 381.30 µl/hr
5 µl 0.33 0.0010 µl/hr 953.17 µl/hr
10 µl 0.46 0.0019 µl/hr 1.901 ml/hr
25 µl 0.73 0.0046 µl/hr 4.775 ml/hr
50 µl 1.03 0.0092 µl/hr 9.551 ml/hr
100 µl 1.46 0.0183 µl/hr 19.153 ml/hr
250 µl 2.30 0.0454 µl/hr 47.532 ml/hr
500 µl 3.26 0.0911 µl/hr 95.492 ml/hr
1000 µlk 4.61 0.0031 µl/min 190.950 ml/hr
1 ml 5.00 0.0033 µl/min 205.30 ml/hr
2 ml 9.0 0.0119 µl/min 747.35 ml/hr
2.5 ml 7.28 to 9.6 0.0076 µl/min 476.21 ml/hr
3 ml 8.66 to 9.0 0.0100 µl/min 11.231 ml/min
5 ml 10.3 to 13.0 0.0208 µl/min 21.781 ml/min
10 ml 14.57 to 15.9 0.0301 µl/min 31.486 ml/min
20 ml 19.13 to 20.05 0.0523 µl/min 54.804 ml/min
30 ml 21.7 to 23.2 0.0673 µl/min 70.518 ml/min
50 ml 26.7 to 32.6 0.1019 µl/min 106.76 ml/min
100 ml 34.9 to 35.7 0.1740 µl/min 182.40 ml/min
140 ml 38.40 0.2106 µl/min 220.82 ml/min
* Note: These figures have been rounded and therefore may not exactly match the Syringe Diameter Chart.
 
 
Common Syringe Data
| TOP |
Diameter & Surface Area
  The following list is a guide to common syringes and their associated diameters and surface area. Syringe diameter, in mm, is listed below for each syringe. All Harvard Apparatus microprocessor syringe pumps require the user to input syringe diameter information. The pump uses this diameter data to set flow rates. The PHD 22/2000 series of syringe pumps also has this information built into the pump memory in a handy Syringe Look Up Table.† Surface area information was used to calculate PSI (pounds per square inch). Average pressures for any syringe pump and syringe combination can be calculated by dividing the average (nominal) syringe pump force by the syringe diameter (in square inches) to obtain PSI. Example, nominal pressure obtained using a 25 ml Hamilton GastightÆ Syringe on a PHD 22/2000 standard pressure syringe pump would be: 50 lbs / 0.644 in2 = 77.6 PSI (5.35 bars).
 
Harvard Apparatus Stainless Steel
Volume Dia. (mm) Surface
Area (in2)
2.5 ml 4.851 0.028647
8 ml 9.525 0.110447
20 ml 19.13 0.445505
50 ml 28.6 0.995760
100 ml 34.9 1.482768
Air-Tite HSW Norm-Ject
Volume Dia. (mm) Surface
Area (in2)
1 ml 4.69 0.026777
2.5 ml 9.65 0.113364
5 ml 12.45 0.188695
10 ml 15.9 0.307763
20 ml 20.05 0.489386
30 ml 22.9 0.638401
50 ml 29.2 1.037978
Becton Dickinson
‘Plasti-pak’
Volume Dia. (mm) Surface
Area (in2)
1 ml 4.78 0.026895
3 ml 8.58 0.097514
5 ml 11.99 0.205735
10 ml 14.43 0.303904
20 ml 19.05 0.494279
30 ml 21.59 0.649601
50 ml 26.59 1.030881
60 ml 26.59 1.030881
Terumo Plastic
Volume Dia. (mm) Surface
Area (in2)
1 ml 4.7 0.026892
3 ml 8.95 0.097514
5 ml 13 0.205735
10 ml 15.8 0.303904
20 ml 20.15 0.494279
30 ml 23.1 0.649601
60 ml 29.1 1.030881
 
SGE Scientific
Glass Engineering
Volume Dia. (mm) Surface
Area (in2)
5µl 0.34 0.000143
10 µl 0.49 0.000286
25 µl 0.73 0.000645
50 µl 1.03 0.001292
100 µl 1.46 0.002584
250 µl 2.30 0.006457
500 µl 3.26 0.012914
1 ml 4.61 0.025827
2.5 ml 7.28 0.064590
5 ml 10.31 0.129176
10 ml 14.57 0.258323
25 ml 23.00 0.643989
50 ml 27.50 0.920636
100 ml 35.00 1.491277
Hamilton Glass -
All Types
Volume Dia. (mm) Surface
Area (in2)
0.5µl 0.103 1.29151E-05
1 µl 0.146 2.59495E-05
2 µl 0.206 5.16603E-05
5 µl 0.326 0.000129
10 µl 0.485 0.000286
25 µl 0.729 0.000647
50 µl 1.03 0.001292
100 µl 1.457 0.002584
250 µl 2.304 0.006462
500 µl 3.256 0.012906
1 ml 4.608 0.025849
1.25 ml 5.151 0.032300
2.5 ml 7.285 0.064607
5 ml 10.3 0.129151
10 ml 14.57 0.258323
25 ml 23.03 0.645838
50 ml 32.57 1.291630
100 ml 32.57 1.291630
 

Cadence Science, Inc.
Formly Popper &
MICRO-MATE Glass

Volume Dia. (mm) Surface
Area (in2)
.25 ml 3.47 0.014658
0.5 ml 3.62 0.015953
1 ml 4.82 0.028282
2 ml 8.91 0.096645
3 ml 8.91 0.096645
5 ml 11.71 0.166931
10 ml 14.65 0.261275
20 ml 19.56 0.465758
30 ml 22.7 0.627298
50 ml 28.02 0.955782
100 ml 35.7 1.551525
Becton Dickinson Glass All-Types
Volume Dia. (mm) Surface
Area (in2)
0.5 ml 4.64 0.026209
1 ml 4.64 0.026209
2.5 ml 8.66 0.091297
5 ml 11.86 0.171235
10 ml 14.34 0.250335
20 ml 19.13 0.445505
30 ml 22.7 0.627298
50 ml 28.6 0.995760
100 ml 34.9 1.482768
Sherwood-Monoject
Plastic
Volume Dia. (mm) Surface
Area (in2)
1 ml 4.65 0.026323
3 ml 8.94 0.097297
6 ml 12.7 0.196350
12 ml 15.9 0.307763
20 ml 20.4 0.506621
35 ml 23.8 0.689567
60 ml 26.6 0.861362
140 ml 38.4 1.795085
 
How to Select the Correct Syringe for Your Application 
W Syringe Specifications
Syringe
Type/Size
Swage
Lock
Luer
Lock
RN Threaded 1/4 • 28 Luer
Slip Fit
Pressure Maximum p.s.i. Compatibility
with
Substance
in Syringe
Accuracy 1% Accuracy 5% Materials
Stainless Steel Syringes
2.5 ml         7,500 Maximum   316 / St. Steel
8 ml         1,500 Maximum   316 / Perfluoroelastomer
20 ml       750 Maximum   316 / Viton or Perfluoroelastomer
50 ml       750 Maximum   316 / Viton or Perfluoroelastomer
100 ml       750 Maximum   316 / Viton or Perfluoroelastomer
200 ml       750 Maximum   316 / Viton or Perfluoroelastomer
Glass GasTight Syringes
1 to 100 µl   1,000 Maximum   Glass and Teflon
250 to 500 µl   500 Maximum   Glass and Teflon
1 to 10 ml     100 Maximum   Glass and Teflon
25 to 100 ml     100 Maximum   Glass and Teflon
Glass Multifit Syringes
2 to 50 ml         100 Maximum   Glass Only
Plastic Syringes
1 ml       125 Minimum   Polypropylene and Natural Rubber
5 ml       125 Minimum   Polypropylene and Natural Rubber
10 ml       125 Minimum   Polypropylene and Natural Rubber
20 ml       125 Minimum   Polypropylene and Natural Rubber
30 ml       125 Minimum   Polypropylene and Natural Rubber
50/60 ml       125 Minimum   Polypropylene and Natural Rubber
140 ml       125 Minimum   Polypropylene and Natural Rubber
 
W Pressure Unit Cross Reference Chart
  atm psi cm H2O mm Hg kPa inch H20 inch Hg mbar
1 atm= 1 14.696 1033.228 760 101.325 406.783 29.921 1013.25
1 psi= 0.068 1 70.307 51.715 6.895 27.68 2.036 68.948
1 cm H2O 0.001 0.0142 1 0.7356 0.0981 0.3937 0.0291 0.9807
1 mm Hg= 0.0013 0.0193 1.36 1 0.133 0.5352 0.039 1.333
1 kPa= 0.0099 0.145 10.197 7500.616 1 4.015 0.295 10
1 inch H2O= 0.0025 0.036 2.54 1.868 0.2491 1 0.0736 2.491
1 inch Hg= 0.03354 0.4912 34.532 25399 3.386 13.595 1 33.864
1 mbar= 0.001 0.015 1.02 0.7501 0.1 0.4015 0.0295 1
 

 Conversion Table
for Force Units

mN mg-force mp
0.1 10 10.2
0.2 20 20.39
0.3 30 30.59
0.4 40 40.79
0.5 50 50.99
0.6 60 61.18
0.7 70 71.38
0.8 80 81.58
0.9 90 91.77
1 100 101.97
2 200 203.94
3 300 305.91
4 400 407.89
5 500 509.86
6 600 611.83
7 700 713.8
8 800 815.77
9 900 917.74
1N= 1 Newton= 1 kg m/s2
1p= 1 Pond
 
 

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