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Home Microincubation / Microinjectors Microincubators Open Perfusion Microincubators


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Open Perfusion Microincubators

The PDMI-2 operates equally well under static or perfused conditions. When operated with perfusion, the micro-incubator’s unique design places flexible tubes carrying inflowing perfusate in contact with a temperature regulated Peltier heat pump driven plate, thus heating or cooling the incoming fluid to a command temperature. This eliminates the need for auxiliary preheating or cooling. The Peltier plate heats or cools the cell carrying central chamber uniformly when no perfusate is flowing.
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Item Listing Full Description Specifications Journal Articles Manuals
Item# Description
650043 PDMI-2 Open Perfusion Micro-Incubator, comes with LU-PD and LU-ASP (Adapter not included, see page M93)
650058 Copper Cooling Tube for PDMI-2

For local pricing, contact your nearest distributor.



Full Description Back to Top

• Heats and cools
• ~5 to 50C
• Maintains required cell environment for hours:
- pH and O2
- temperature
• Peltier heat pump
• Flexible chamber option
• Easily change perfusion fluid
• Makes temperature cycle experiments easy

The PDMI-2 operates equally well under static or perfused conditions. When operated with perfusion, the micro-incubator’s unique design places flexible tubes carrying inflowing perfusate in contact with a temperature regulated Peltier heat pump driven plate, thus heating or cooling the incoming fluid to a command temperature. This eliminates the need for auxiliary preheating or cooling. The Peltier plate heats or cools the cell carrying central chamber uniformly when no perfusate is flowing.

Flexible Temperature Range
The operating temperature range of the PDMI-2 Open Perfusion Micro-Incubator is nominally ~5 to 50C when used with the TC-202A Temperature Controller. Lower temperatures can be achieved (approximately 10 below ambient) by using an optional cool tube water-carrying coil which attaches to the perimeter of the PDMI-2. Temperatures stabilize quickly throughout the operating range; temperature uniformity across the chamber is excellent.

Optimal and Flexible Perfusion
Two temperature controlled perfusion lines deliver perfusates through the micro-incubator into the chamber a nominal rate of 3 ml per minute each. Fluid level is maintained by placing an aspirator (model LU-ASP) in the chamber. Fluid height is easily adjusted via a simple screw mechanism; a cleverly designed suction port eliminates fluid flutter thus making aspiration quiet; a magnetic base secures the aspirator to the micro-incubator at a user selectable convenient location.

Excellent pH Control
The PDMI-2 also provides gas flow over the top of the chamber for pH control and improved temperature uniformity. The open design provides excellent mechanical and optical access to the media.

Low Noise Conditions
A PTFE well is provided with an Ag/AgCl electrode at its base to allow for the formation of a salt bridge when making low noise electrophysiological recordings.


Specifications Back to Top

Specifications650043650058
ModelPDMI-2 
CertificationsCE 
Height Metric17 mm*na mm
Height English0.67 in*na in
Weight Metric0.5 kg*na kg
Weight English17.9 oz*na oz
Diameter Metric152 mm*na mm
Diameter English6 in*na in
Chamber OptionsCorning 35 mm Petri dishes, LU-CSD, MSC-TD or MSC-PTD 
Recommeded Gas Flow0.5 to 2.0 
Temperature Range Metric~5 to 10C below ambient to 50C without water cooling 
Temperature Stability Metric0.2C with TC-202A, at 37C with 1 ml/min perfusion 
Temperature Gradient Across Chamber2C with 1.5 ml media volume, 1 ml/min perfusion 
Built-in Temperature SensorThermistor, 100 k? at 25C - YSI 44011 
Peltier Device Current Rating6 A maximum 
Media Perfusion RatesUp to 3.0 ml/min*na ml/min
Microscope Stage MountingCall Harvard Apparatus for options 

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Journal Articles Back to Top

Displaying 1 to 10 of 10results for Open Perfusion Microincubators  

Score

Document

Description

100% Login to view LACK OF RESPONSE SUPPRESSION FOLLOWS REPEATED VENTRAL TEGMENTAL CANNABINOID ADMINISTRATION.pdf
[333 KB]
Lack of response suppression follows repeated ventral tegmental cannabinoid administration: An In Vitro Electrophysiological Study
100% Login to view Permeation and Activation of the M2 Ion Channel.PDF
[248 KB]
Permeation and Activation of the M2 Ion Channel of Influenza A Virus with use of Fast Step Perfusion System
93% Login to view WR19FA 1.PDF
[220 KB]
Permeation and Activation of the M2 Ion Channel of Influenza A Virus with use of Fast Step Perfusion System
91% Login to view PP57 Quantification of Cerebral Perfusion With Real-Time Contrast-Enhanced Ultrasound.pdf
[210 KB]
Respirator pump use in finding Quantification of Cerebral Perfusion With "Real Time" Contrast-Enhanced Ultrasound
54% Login to view HS15 Myoglobin facilitates oxygen diffusion.pdf
[631 KB]
Isolated Heart Apparatus use in Myoglobin facilitating oxygen diffusion
46% Login to view PP172.pdf
[133 KB]
Peak muscle perfusion and oxygen uptake in humans: importance of precise estimates of muscle mass
46% Login to view PP57 Respiratory Pump 57.pdf
[209 KB]
Respirator pump use in finding Quantification of Cerebral Perfusion With "Real Time" Contrast-Enhanced Ultrasound
43% Login to view Noninvasive measurement of the pH of the endoplasmic reticulum.PDF
[372 KB]
Noninvasive measurement of the pH of the endoplasmic reticulum at rest and during calcium release
35% Login to view HS04 latex balloon.pdf
[76 KB]
Latex Balloon use in finding Benefical Effect of Glucose on Short-Term, Perfusion-Pressure Induced Changes of Contractile Efficiency in Isolated Rabbit Hearts
14% Login to view HS04 Contractile Efficiency in Hibernating Myocardium.pdf
[79 KB]
Latex Balloon use in finding Benefical Effect of Glucose on Short-Term, Perfusion-Pressure Induced Changes of Contractile Efficiency in Isolated Rabbit Hearts
   

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Manuals Back to Top

Displaying 1 to 3 of 3results for Open Perfusion Microincubators  

Score

Document

Description

100% Login to view MicroIncubator PDMI-2.pdf
[193 KB]
Model PDMI-2 Micro Incubator Manual
80% Login to view Perfusion Tissue Systems Guide.pdf
[105 KB]
New BS4_K_Isolated Organ.qxd
53% Login to view IH-SR Brochure.pdf
[7 MB]
Isolated Heart Perfusion System for Small Rodents IHSR IH-SR
   

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