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Home Microincubation / Microinjectors Recording and Imaging Chamber Accessories RC Series Chambers


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RC Series Chambers

The RC Series are open bath chambers suitable for physiological experiments performed with inverted or dissecting microscopes including patch clamping, recording from oocytes, isolated perfused tubule, and cell impalement. They have also been used for perfusion in fluorescence microscopy. RC Series chambers are made from polycarbonate. Models vary in bath diameter. A #2 thickness coverslip, 24 x 60 mm (user attached) becomes the floor of the bath. The coverslip is attached with vacuum grease or more permanently with Sylgard or RTV cement.
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Item Listing Full Description Specifications Journal Articles Manuals
Item# Description
640306 Open Bath Chamber, with10 mm Bath
640307 Open Bath Chamber with 11 mm Bath
640308 Open Bath Chamber with 13 mm Bath
640309 Open Bath Chamber with 16 mm Bath

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Full Description Back to Top


The RC Series are open bath chambers suitable for physiological experiments performed with inverted or dissecting microscopes including patch clamping, recording from oocytes, isolated perfused tubule, and cell impalement. They have also been used for perfusion in fluorescence microscopy. RC Series chambers are made from polycarbonate. Models vary in bath diameter. A #2 thickness coverslip, 24 x 60 mm (user attached) becomes the floor of the bath. The coverslip is attached with vacuum grease or more permanently with Sylgard or RTV cement.
Perfusion can be gravity-fed and removed via suction, or alternatively, input and output can be pump-controlled. The perfusion input accepts standard 1.57 mm OD [PE-160] polyethylene tubing. Solutions are removed from a separate suction reservoir to minimize noise in the recording. The suction tube is adjustable to control the bath solution level. Flow rates can exceed 10 ml/min with gravity feed. Multiple perfusion lines can be connected using one of the MP series manifolds in place of the single line connector supplied with the chamber. An isolated reservoir is provided for an agar bridge in the recording ground circuit. An additional agar bridge well (for oocyte recording) can be specified at no additional cost.
RC-10
This small bath model with 10 mm bath diameter is recommended where small volumes and fast solution exchange is important. Bath volume is 160 µl with 2 mm solution height. A single agar bridge well is supplied on this model.
RC-11, RC-13 & RC-16
These larger bath chambers are used for studies of oocytes, slices and other larger preparations. Models RC-13 and RC-16 may also be used in patch clamp experiments where cultured coverslips are placed in the bath. RC-13 accepts 12 mm diameter coverslips and RC-16 accepts 15 mm diameter coverslips. A groove is milled in the chamber underside to hold the coverslip in place.
Note: The double thickness of glass (bottom + sample coverslips) may not be compatible with some short working distance microscope optics.
Mounting the Chamber
RC series chambers are mounted on a microscope with one of the RC Series Stage Adapters.
These chambers are supplied with one Single Input Perfusion Connector, one Suction Tube and one package of 64-0710 #2 Coverslips (24 x 60 mm). Additional coverslips are also available.


Specifications Back to Top

Specifications640306640307640308640309
ModelRC-10RC-11RC-13RC-16

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Journal Articles Back to Top

Displaying 1 to 10 of 10results for RC Series Chambers  

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Document

Description

100% Login to view PP35 Peristaltic Pump.pdf
[381 KB]
Peristaltic pump use in finding Improvements to parallel plate flow chambers to reduce reagent and cellular requirements
85% Login to view 96well eq dialplate2.pdf
[40 KB]
In Vitro Assessment of Plasma Protein Binding and Metabolic Stability of PTK 0796 (BAY 73-6994)
70% Login to view 96well eq dialplate4.pdf
[1.9 MB]
Kringle 5 of Human Plasminogen Induces Apoptosis of Endothelial and Tumor Cells through Surface-Expressed Glucose-Regulated Protein 78
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[1.4 MB]
Three-dimensional Structure of Guanylyl Cyclase Activating Protein-2, a Calcium-sensitive Modulator of Photore
70% Login to view micro eq dial2.pdf
[1.1 MB]
Control of electrostatic interactions between F-actin and genetically modified lysozyme in aqueous media
51% Login to view HS13 Force Transducer.pdf
[160 KB]
Force Transducer use in Cardioselective KATP Channel Blockers Derived from a New Series of Anisamidoethylbenzenesulfonylthioureas
51% Login to view HS13 Cardioselective KATP Channel Blockers.pdf
[165 KB]
Force Transducer use in Cardioselective KATP Channel Blockers Derived from a New Series of Anisamidoethylbenzenesulfonylthioureas
35% Login to view dispo eq dial3.pdf
[868 KB]
An mRNA structure that controls gene expression by binding S-adenosylmethionine
35% Login to view 96well eq dialplate5.pdf
[317 KB]
Interspecies differences in plasma protein binding of MS-275, a novel histone deacetylase inhibitor
30% Login to view micro eq dial1.pdf
[354 KB]
Equilibrium Dialyzer for use in finding Differential ATP binding and Intrinsic ATP Hydrolysis
   

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Manuals Back to Top

Displaying 1 to 5 of 5results for RC Series Chambers  

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100% Login to view Nanoleader Pump Series NLS110P Pressure Pump Manual.pdf
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705000 NLS110P Pump Manual
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Model 44 Syringe Pump Series Manual
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Peristaltic Pump 1200 Series Manual
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Series 1400 Pulsatile Blood Pumps Manual
   

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