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Home Dialysis / Sample Prep / SPE Liposome Products Mini LipoPrep for the Preparation of Small Volume Liposomes


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Mini LipoPrep for the Preparation of Small Volume Liposomes

Today liposomes are an important part of biological, pharmaceutical, and medical research. Because liposomes are the most effective
carriers for the introduction of many different types of agents into cells, the applications of liposome-based samples and products are extremely wide.
Mini Lipoprep
• The Mini Lipoprep is well suited for the preparation of small volumes of liposomes (0.5 ml to 1.0 ml).
• It consists of a sample chamber, which is used with a membrane, and a motor to rotate the sample chamber at constant speed.
• Liposomes are formed during dialysis of the detergent in the lipid/detergent micelles through the membrane.
• Using the Mini Lipoprep, unilamellar homogeneous liposomes can be prepared in 2 to 3 hours.

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Item Listing Full Description Specifications Journal Articles Manuals
Item# Description
742100 Membranes for Multi-Equilibrium DIALYZERS and/or Mini Lipoprep, 5000 Dalton MWCO Membrane, Qty. of 200
742102 Membranes for Multi-Equilibrium DIALYZERS and/or Mini Lipoprep, 10000 Dalton MWCO Membrane, Qty. of 200

For local pricing, contact your nearest distributor.



Full Description Back to Top

Advantages
• Fast, easy to use
• Unilamellar
• Reproducible
• Uniformly-sized (25 to 800nm)
• Very high encapsulation
• Low lipid loss (1 to 2%)
• Stable liposomes (one year or longer)
Applications
• Drug delivery
• DNA delivery and gene therapy
• Cell-cell interactions
• Cosmetics
• Diagnostics

Today liposomes are an important part of biological, pharmaceutical, and medical research. Because liposomes are the most effective
carriers for the introduction of many different types of agents into cells, the applications of liposome-based samples and products are extremely wide. A few examples are presented below:
• Liposomes are used for studying protein-liposome interactions with compounds such as serum lipoproteins, lectins, toxins and clotting components.
• They are used for the entrapment of anti-tumor agents, anti-microbial drugs, anti-inflammatory and immunomodulatory agents and CNS-active drugs. The therapeutic applications are presently include cancer therapy, arthritis, metal chelation therapy, enzyme replacement therapy, hemophilia (factor VIII), myocardial infarction, and as radiopharmaceutical markers.
• Liposomes are used for studying in vitro and in vivo liposome-cell interactions.
• They are used for reconstitution experiments especially for ion transport systems.
• In molecular biology, liposomes are used for the mediated delivery of macromolecules into eukaryotic cells.
• In immunology, antigens encapsulated in liposomes are used to generate antibodies, to mediate active and passive immunization and for many other applications.
• The use of liposomes in gene-based technology and in broader biotechnology and pharmaceutical applications is increasing day by day.
Harvard Apparatus offers two instruments, the Mini Lipoprep and the Liposomat, for fast, easy and reproducible liposome preparation. No prior experience in liposome preparation is necessary. Using these systems, liposomes can be prepared with virtually any lipid, depending on the specifications of the experiment and the types of biomolecules that need to be encapsulated. Liposome preparation with these instruments is highly reproducible and compared to other liposome preparation methods on the market, these liposomes are very stable. Under sterile conditions, liposomes prepared using the Mini Lipoprep and Liposomat can be stored for one year or longer.
Liposome Specifications
With a few exceptions, liposomes are composed of natural or synthetic phospholipids, mostly lecithins. Hence, they can be metabolized in vivo and are generally non-toxic and non-antigenic.
Agents can be entrapped in liposomes without the formation of chemical bonds and sensitive molecules can be protected within them. Because of their restricted permeability, liposomal preparations offer a controllable, time-dependent release system.
The entrapment of a drug within liposomes changes its pharmaco-kinetics and can result in a better therapeutic index and enhanced cellular uptake. Since different agents need different lipid agents to create an optimal coat, in vivo experiments following liposome preparation are often the optimal means of determining which lipid represents the best compromise between permeability and in vivo stability for any given agent.
Stability of Liposomes
No general rule for maintaining liposome stability exists since it depends on several parameters, such as the type of lipid used, the properties of the drug/agent in the liposomes, their size, lamellarity, and homogeneity, the electrolyte content and pH of the medium used, and also on the specifications of the desired application.
Size Control
The size of liposomes can be adjusted experimentally by varying several parameters: the dialysis rate, type of detergent, type of lipid(s), lipid/detergent molar ratio, lipid concentration, electrolyte content and pH.
Detergents
The detergents used are gentle in their action and are not expected to hydrolyze or peroxidize liposome components. The most frequently used detergents are sodium cholate, n-octyl-b-D-glucopyranoside and n-octyl-tetraoxyethylene (POE4). Other detergents are sodium salts of glycocholic acid, deoxycholic acid, taurocholic acid, chenoxycholic acid, n-hexyl- and n-heptyl-glucopyranoside and lauryldimethylamine oxide.


Specifications Back to Top

Specifications742100742102
Level 4Membranes for Mini LipoPrepMembranes for Mini LipoPrep
MWCO5,00010,000
MWCO UOMDaltonDalton

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Journal Articles Back to Top

Displaying 1 to 10 of 43results for Mini LipoPrep for the Preparation of Small Volume Liposomes   Page 1  2  3  4  5 

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Document

Description

100% Login to view lipomat.pdf
[66 KB]
LipoPrep Instrument used in Engineering Porphyrin Encapsulated Vesicles for Metal Removal from Wastewater
93% Login to view HS20 Mini Ventilator.pdf
[681 KB]
Mini Ventilator used in Nitric oxide prevention of cardiovascular disease
91% Login to view VT06 Ventilator use in finding load as an acute determinant of end-diastolic pressure-volume relation.pdf
[200 KB]
h4Ventilator use in finding load as an acute determinant of end-diastolic pressure-volume relation010100051p
78% Login to view VT12 mini 2002hs.pdf
[177 KB]
Small rodent ventilator use in finding chronic measurement of cardiac output in conscious mice
70% Login to view Airway Surface Liquid Osmolality Measured using Fluorophore-encapsulated Liposomes.pdf
[258 KB]
Airway Surface Liquid Osmolality Measured during Fluorophore-encapsulated Liposomes
70% Login to view PDMI03 2003microinc.pdf
[251 KB]
Airway Surface Liquid Osmolality Measured during Fluorophore-encapsulated Liposomes
62% Login to view VT23 Inhibition Shapes Frequency Response Areas with Small Animal Ventilator.pdf
[673 KB]
Inhibition Shapes Frequency Response Areas with Small Animal Ventilator
58% Login to view VT11 Effects of Concentrated Ambient Particles in rats and Hamsters with Small Animal Respirator.pdf
[874 KB]
Effects of Concentrated Ambient Particles in rats and Hamsters with Small Animal Respirator
53% Login to view VT26 Mini Ventilator 845.pdf
[1.2 MB]
Mini Ventilator use in finding Heterozygous deficiency of hypoxia-inducible factor 2a
38% Login to view PP169.pdf
[186 KB]
New Method of Embolus Preparation for Standardized Embolic Stroke in Rabbits
   

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Manuals Back to Top

Displaying 1 to 4 of 4results for Mini LipoPrep for the Preparation of Small Volume Liposomes  

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100% Login to view Mini Peristaltic Pump.pdf
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Mini Peristaltic Pump Manual
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[1.8 MB]
Guide to Cell Modification and Re-Engineering Solutions
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Model 680 Small Animal Ventilator Manual
56% Login to view 550000 Ventilator 683 Manual.pdf
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Model 683 Small Animal Ventilator User's Manual
   

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