Welcome to Harvard Apparatus UK! Login / Register
Search

Search for these keywords
Home Catalogs Online Technical Resources My Account
Advanced Search   
Products by Category

Featured Products
Warner Cell Electrophysiology & Cell Biology Research
Hugo Sachs Elektronic Isolated Organ and Tissue Systems
BTX Electroporation and Electrofusion Products
Panlab Behavior Research Products
Hoefer Electrophoresis Products
Coulbourn Instruments
Clark Glass Products
Navicyte Ussing/Diffusion Chambers
CMA
Harvard Apparatus
 

Home Cell Biology Products Perfusion Valve Controllers Fast-Step Valve Control Perfusion Systems


Larger Views

 

Fast-Step Valve Control Perfusion Systems

The SF-77B is a simple but highly effective stimulus solution delivery device for use in a variety of patch clamp and electrophysiology studies. Control and test solutions flow continuously through adjacent stimulus delivery tubes and a stepper mechanism selects which tube is directed at the preparation. The rapid response and nominal hysteresis of the stepper mechanism allows for very fast switching times. Complete solution changes are typically achieved within 20 msec for a large 700 mm step and times are significantly shortened as the step size is decreased.
Search for Product in current catalog
Item Listing Full Description Specifications Journal Articles Manuals
Item# Description
640114 Model SF-77B Standard System with MM Series Manifolds, 1 pkg. 3SG700-5 Glass, GH-1 Glass Holder, and 1 pkg. each PE-10 and PE-50 Tubing
640115 Model SF-77BST Theta Glass System with MM Series Manifolds, 1 pkg. TGC-200-10 Glass, GH-2T Glass Holder, and 1 pkg. each PE-10 and PE-50 Tubing
640116 Model SF-77BLT Large Tube System with ML Series Manifolds, 1 pkg. SG1000-5 Glass, GH-10 Glass Holder, and 2 pkg. of PE-50 Tubing

For local pricing, contact your nearest distributor.



Full Description Back to Top

• Solution stimulus delivery for patch clamp and other electrophysiology studies
• Solution changes in milliseconds
• Minimal flow turbulence
• No switching through intervening solutions
• Manual or automatic step control (digital or analog)
• Modest cost and easy maintenance
• Use with most chambers or culture dishes

The SF-77B is a simple but highly effective stimulus solution delivery device for use in a variety of patch clamp and electrophysiology studies. Control and test solutions flow continuously through adjacent stimulus delivery tubes and a stepper mechanism selects which tube is directed at the preparation. The rapid response and nominal hysteresis of the stepper mechanism allows for very fast switching times. Complete solution changes are typically achieved within 20 msec for a large 700 mm step and times are significantly shortened as the step size is decreased.
Multiple Solution Studies
In the standard configuration, up to six different solutions are connected to a single input manifold which in turn is connected to one of three square glass stimulus ports. The three stimulus port design is superior to a two-port design in that complex solution exchange protocols can be brought to bear on the sample under study. Since the complete system is designed to accommodate three manifolds (one for each stimulus port) and each manifold can accommodate up to 6 feed lines, it is possible to immediately select between 18 different input solutions.
• Solution changes between stimulus ports occur within milliseconds
• Changes between solutions connected to individual port occur within 5 secs.
• Entirely new solutions can be added into any port with a waiting time of no more than 30 seconds.
• Cell is never required to pass through intervening solutions to get from control to test solution
Manual or External Control
The stepper mechanism can be manually controlled via the front panel or externally directed from your data acquisition program. Manually, the system can be stepped to 8 positions in 7 equally spaced steps. These same 8 positions can also be directly selected by applying an analog signal to the external analog input BNC or by passing a 3 byte word to the TTL input on the instrument rear panel.
Square Glass Stimulus Ports
The square glass tubes used for solution delivery significantly reduces mixing turbulence between solution flows allowing the SF-77B to be used for studies with both membrane patches and whole cells, even when the cells are not fixed to a substrate.
System Versatility
The design of the SF-77B permits the use of various size glass tubing for perfusion delivery.
SF-77B:
Standard System
(0.7 mm ID tubes)
The standard system is shipped with 3SG700-5 single-walled 3-barrel glass tubing which eliminates the need to glue individual barrels together. Spacing between barrels is 0.7 mm and step speed between adjacent barrels is typically 20 msec. Single barrel SG800-5 tubes (up to 5) can be used with the same holder.
SF-77BLT:
Large Tube System
(1.0 mm ID tubes)
Larger stimulus ports are required when using the SF-77B with larger cell structures such as the Xenopus oocyte. Solutions are delivered through 1.0 mm ID square tubes (SG1000-5) with barrel-to-barrel spacing of 1.4 mm.
SF-77BST:
1 msec Stepping
With Theta Tubing
The SF-77B has been successfully tested using theta style capillary tubing with a step speed of 1 msec between adjacent barrels. Standard 2 mm diameter theta tubing is pulled to a tip diameter of 300 mm with a spacing of 100 mm between barrels. In this design, placement of the pipette tip is critical due to the smaller perfusion stream. Since mechanical artifacts can be evident when using these small step sizes the motor drive voltage is adjustable at the rear panel.
Easy Set-Up
The stepper mechanism is compact, lightweight, and free of either mechanical or electrical noise. The mechanism connects to the control box with a 2 meter shielded cable and is provided with a mounting rod for attachment to a manipulator. Manifolds can support 2,4 or 6 inputs depending on the experiment. Solutions flow from reservoirs to the manifold through PE-50 tubing and PE-10 tubing is used to connect the manifold outputs to the glass tubes. We designed the VC-6M to accompany the SF-77B stepper. It conveniently fits inside a Faraday enclosure and is remotely controlled with the VC-6 controller. When purchased with the SF-77B, the complete package is discounted, see pages M136 and M137 for details.
These Fast-Step Systems are supplies with one 2 to 1 and two 6 to 1 manifolds. Other manifolds are available separately, see page M140. Note: Model SF-77B/5M: SF-77B may also be ordered as a 5 manifold system. Consult our technical support department for details.


Specifications Back to Top

Specifications640114640115640116
ModelSF-77BSF-77BSTSF-77BLT
CertificationsCECECE
Fast-Step System Height Metric6.3 cm6.3 cm6.3 cm
Fast-Step System Width Metric14 cm14 cm14 cm
Fast-Step System Depth Metric23 cm23 cm23 cm
Fast-Step Weight Metric2.7 kg2.7 kg2.7 kg
Number of Steps1 to 7 (2 to 8 positions)1 to 7 (2 to 8 positions)1 to 7 (2 to 8 positions)
Step SizeAdjustable from 100 m to 1.5 mm in 100 m increments, with STEPS/POSITION switchAdjustable from 100 m to 1.5 mm in 100 m increments, with STEPS/POSITION switchAdjustable from 100 m to 1.5 mm in 100 m increments, with STEPS/POSITION switch
Step SpeedTypically 20 msec for 700 m stepTypically 20 msec for 700 m stepTypically 20 msec for 700 m step
Step Control: Manual8 positions with POSITION selector8 positions with POSITION selector8 positions with POSITION selector
Step Control: Analog Signal5 positions with voltage levels 0-4 V, 1V/step5 positions with voltage levels 0-4 V, 1V/step5 positions with voltage levels 0-4 V, 1V/step
Step Control: Digital Signal8 positions with 3 byte TTL signal8 positions with 3 byte TTL signal8 positions with 3 byte TTL signal
Max. Stepped Range12.5 mm12.5 mm12.5 mm
Mounting Handle Diameter Metric6.3 mm6.3 mm6.3 mm
Mounting Handle Length Metric10 cm10 cm10 cm
Stepper Weight (including handle) Metric110.5 g110.5 g110.5 g
Solution ManifoldsThree manifolds supplied with each system; MM series for SF-77B and SF-77BST and ML series with SF-77 BLT; Manifolds have 2, 4 or 6 inputs, specify your choice when ordering
MM Series MM-2, MM-4 and MM-6 manifolds use PE-50 tubing.
Three manifolds supplied with each system; MM series for SF-77B and SF-77BST and ML series with SF-77 BLT; Manifolds have 2, 4 or 6 inputs, specify your choice when ordering
MM Series MM-2, MM-4 and MM-6 manifolds use PE-50 tubing.
Three manifolds supplied with each system; MM series for SF-77B and SF-77BST and ML series with SF-77 BLT; Manifolds have 2, 4 or 6 inputs, specify your choice when ordering
MM Series MM-2, MM-4 and MM-6 manifolds use PE-50 tubing.
Solution Flow Rates MetricRates measured with solution reservoir height of approx. 60 cm (24 in)
With MM Series 100 l/min
With ML Series 1 ml/min
Rates measured with solution reservoir height of approx. 60 cm (24 in)
With MM Series 100 l/min
With ML Series 1 ml/min
Rates measured with solution reservoir height of approx. 60 cm (24 in)
With MM Series 100 l/min
With ML Series 1 ml/min

Back to Top



Journal Articles Back to Top

Displaying 1 to 7 of 7results for Fast-Step Valve Control Perfusion Systems  

Score

Document

Description

100% Login to view Permeation and Activation of the M2 Ion Channel.PDF
[248 KB]
Permeation and Activation of the M2 Ion Channel of Influenza A Virus with use of Fast Step Perfusion System
93% Login to view WR19FA 1.PDF
[220 KB]
Permeation and Activation of the M2 Ion Channel of Influenza A Virus with use of Fast Step Perfusion System
16% Login to view PP69 PHD 2000 Syringe Pump.pdf
[598 KB]
Passive mixing in a three-dimensional serpentine microchannel - Microelectromechanical Systems, Journal of
13% Login to view PP33PR 1.PDF
[393 KB]
Micro/nano spacecraft thermal control using a MEMS-based pumped liquid cooling system with use of Programmable syringe pump
12% Login to view HS04 latex balloon.pdf
[76 KB]
Latex Balloon use in finding Benefical Effect of Glucose on Short-Term, Perfusion-Pressure Induced Changes of Contractile Efficiency in Isolated Rabbit Hearts
11% Login to view HB03 Leukotriene B4 Promotes Reactive Oxidant Generation and Leukocyte Adherence During Acute Hypoxia.pdf
[198 KB]
Homeothermic Blanket Leukotrience B4 promotes reactive oxidant generation and leukocyte adherence during acute hypoxia
11% Login to view HB08 mri bkt 3.pdf
[272 KB]
VEGF antagonism reduces edema formation and tissue damage after ischemia/reperfusion injury in the mouse brain
   

Back to Top



Manuals Back to Top

Displaying 1 to 1 of 1result for Fast-Step Valve Control Perfusion Systems  

Score

Document

Description

100% Login to view Perfusion Tissue Systems Guide.pdf
[105 KB]
New BS4_K_Isolated Organ.qxd
   

Back to Top

Breakthrough Products for Regenerative Medicine
2011 Regenerative Medicine Brochure
100's of Innovative products developed with leading scientists & surgeons!
  • Injection & Perfusion
  • Bioreactors
  • Biosensing
  • Total Solutions
  • Total Support


Announcing the new
PHD ULTRA™ Syringe Pumps

Click here for more information about PHD ULTRA™ Syringe Pumps and to download a brochure.

  • Patent pending mechanical design provides highest accuracy/precision & smoothest flow
  • Easy operation with advanced touch screen
  • Advanced EZ PRO™ software for PC functions without a PC.


Sign-up for a FREE Full Line Bioresearch Catalog
CLICK HERE!

  • Over 11,000 specialty products to enhance your bioresearch
  • Enhanced solutions for small animal research
  • 100's of pages of specialized information on instrumentation, applications, uses of products

 

Products on this website are for Research Use Only. Not for use in humans unless
proper investigational device regulations have been followed.


Privacy Policy   |   Terms and Conditions   
Copyright© Harvard Apparatus Ltd. - All rights reserved.
(44) 1223-423723
Website-related comments or concerns can be emailed to our webmaster.

Anesthesia Equipment Animal Handling Products Behavior Research Products Biological Measurement Products Cardiovascular Products Cell Biology Products Microincubation / Microinjectors CPK Atomic Models Delivery & Sampling Products Dialysis / Sample Prep / SPE Electroporation / Transfection Electrophysiology Products General Laboratory Products Homeothermic/Temperature Control Isolated Organ & Isolated Tissue Molecular Biology Products Neuroscience Products Non-Invasive Blood Pressure Physiology Products Respiratory Products Stereotaxic Instruments Surgical Equipment Surgical Instruments Syringe Pumps/Peristaltic Pumps Tubing & Connectors Ventilators