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Home Cell Biology Products Tissue/Cell Culture Equipment Horizontal Ussing/Diffusion Chamber System Accessories


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Horizontal Ussing/Diffusion Chamber System Accessories

The Horizontal Diffusion Chamber System is designed for transport and toxicology studies using cells and tissues which are exposed to an air interface which occurs in the normal in vivo environment such as with nasal, pulmonary, corneal, or dermal cells. The chambers create an environment in which the apical surface of the tissue or cell monolayer is exposed to liquids, semi-solid compounds or gases while the basolateral surface is perfused with medium.
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Item Listing Full Description Journal Articles Manuals
Item# Description
660017 Heat Block for Horizontal Chambers, for 1 to 6 Chambers
660053 Closed Screws, used when perfusion is not needed, pkg. of 24
660054 Closed Caps for Horizontal Chambers
660055 Open Caps for Horizontal Chambers
660056 Male Luer Fitting - 3/32 Bar, pkg. of 24
660022 Tissue Mounting Ring for Horizontal Chamber Only
660058 Open Screw (straight hole) and O-rings, serves as an electrode holder for Horizontal Diffusion Chambers, pkg. of 24

For local pricing, contact your nearest distributor.



Full Description Back to Top

• Designed for transport studies on cells and tissues which are exposed to an air interface — nasal, corneal, pulmonary or dermal
• Option of using cultured cells or tissue samples makes the horizontal diffusion chamber system versatile and convenient for correlation studies
• Six simultaneous tests can be run, improving experimental precision
• Heat block allows system temperature control with the use of a circulating water bath
• Option of working in open or closed configuration
• Compact configuration 38.1 x 8.9 x 10.2 cm (15 x 3.5 x 4 in) conserves valuable bench space

The Horizontal Diffusion Chamber System is designed for transport and toxicology studies using cells and tissues which are exposed to an air interface which occurs in the normal in vivo environment such as with nasal, pulmonary, corneal, or dermal cells. The chambers create an environment in which the apical surface of the tissue or cell monolayer is exposed to liquids, semi-solid compounds or gases while the basolateral surface is perfused with medium.
The Horizontal Diffusion Chamber System consists of six horizontal chambers, and six closed open chamber tops into which either Snapwell™ devices or tissue mounting rings may be fitted on a horizontal plane, and a heat block. The closed perfusion system also accepts optional diffusion chamber electrodes for resistance measurements and electrophysiology. Either a peristaltic or syringe pump (not included with the system, see Section A in our Full-Line Bioscience Catalog) can be used to perfuse the lower surface of the Snapwell™ insert, usually the basolateral surface. The apical (upper) surface of the diffusion chamber can be used in either the open or closed configuration.
Open Configuration: The upper (apical) surface of the cells is directly accessible, making the system suitable for drug transport or cytotoxicity testing of liquids and semi-solid materials.
Closed Configuration
Cells or tissue can be exposed to solutions, perfused with gas or liquid, or gas pressure can be applied. The closed perfusion system also accepts Diffusion Chamber Electrodes for resistance measurements and electrophysiology.
Diffusion Chamber Electrodes
Diffusion Chamber Electrodes, see diagram on page 191, can be used in both Vertical and Horizontal Diffusion Chambers for making monolayer resistance measurements and electrophysiological determinations. The 2 mm pin connections are compatible with our electrical manifolds that connect the electrodes to various models of Current/Voltage Clamps, see pages M176 to M182. The silver/silver chloride reference electrode consists of a chlorided silver wire contained in a glass barrel that terminates in a ceramic tip. The barrel is filled with any suitable electrolyte solution, usually the buffer used for the external media or 3M KCl, and is refillable. The microporous ceramic tip limits the flux of ions into and out of the electrodes, making them a suitable replacement for bridge systems in most applications. The electrode connects to a wire 1 m in length and is terminated with a 2 mm gold plated pin. We offer a single horizontal diffusion chamber and a 6-chamber system. The system includes the following components:
Catalog No. Qty. Product
66-0017 1 Heat Block for Horizontal Chambers, for 1 to 6 Chambers
66-0016 6 Horizontal Diffusion Chambers


Journal Articles Back to Top

Displaying 1 to 10 of 30results for Horizontal Ussing/Diffusion Chamber System Accessories   Page 1  2  3 

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Description

100% Login to view PP124.pdf
[441 KB]
Imaging cocaine-induced changes in the mesocorticolimbic dopaminergic system of conscious rats
78% Login to view WR16 RC25.pdf
[275 KB]
Recording Chamber use in finding Hypoxic Augmentation of Fast-Inactivating and Persistent Sodium Currents in Rat Caudal Hypothalamic Neurons
73% Login to view OT31.pdf
[673 KB]
Development of a multiplexed microbioreactor system for high throughput bioprocessing
70% Login to view Hypoxic Augmentation of Fast-Inactivating and Persistent Sodium.pdf
[281 KB]
Recording Chamber use in finding Hypoxic Augmentation of Fast-Inactivating and Persistent Sodium Currents in Rat Caudal Hypothalamic Neurons
70% Login to view OT01 Chambers.pdf
[963 KB]
Primary Uroepithelial Cultures
70% Login to view Primary Uroepithelial Cultures.pdf
[971 KB]
Primary Uroepithelial Cultures
67% Login to view PP192.pdf
[451 KB]
Multiple sprayer system for high-throughput electrospray ionization mass spectrometry
61% Login to view Permeation and Activation of the M2 Ion Channel.PDF
[248 KB]
Permeation and Activation of the M2 Ion Channel of Influenza A Virus with use of Fast Step Perfusion System
59% Login to view electroprep1.pdf
[232 KB]
Isolation and purification of two major serum amyloid A isotypes SAA1 and SAA2 from the acute phase plasma of mice
57% Login to view TD0160 1.PDF
[348 KB]
Isotomic Transducer use in Stabilization of Temporally Summated Muscle Contractions in the Lobster (Panulirus) Pyloric System
   

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Manuals Back to Top

Displaying 1 to 1 of 1result for Horizontal Ussing/Diffusion Chamber System Accessories  

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100% Login to view Ussing Chamber Systems.pdf
[1.5 MB]
Guide to Ussing Chamber Systems
   

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