Model BSC-PC Prechamber (for holding slices) Tissure Slice Chamber
This unique and versatile Brain/Tissue Slice Chamber System offers the investigator two in vitro methods of studying a thin slice of tissue, which is bathed by perfusates of interest. The BSC-HT (Haas Top) is an interface-type chamber in which one cut surface of the slice is wetted by a perfusing solution while the other surface is kept moist by a humidified gas mixture. The BSC-ZT (Zbicz Top) is a chamber in which solutions flow transversely to fully submerged slices. Each top unit allows for rapid changeover of fluids which are being investigated and both provide for excellent access to the slices for electrophysiological recording or other observations. Search for Product in current catalog
• Maintains brain slices for 10 hours or more on the bench-top • Modular design accommodates submersible or interface chambers • 20 years of use in leading labs • Sensitive recordings without electrical interference
The BSC-PC chamber allows several (10 to 12) brain slices to be stored in vitro for several hours while maintaining excellent viability. A mixture of 95% O2 /5% CO2 is bubbled through physiological saline. This initiates a circular flow and oxygen enriched saline which continuously permeates the slices. Slices remain viable for hours while awaiting transfer to an examining chamber. Gas flow pressure should not exceed 5 p.s.i.