• Operates with 1 or 2 oxygen electrodes
• Interfaces to respirometry/monitoring software, or use as stand alone meter
• Use any unit of PO2 or oxygen concentration
• Closed cell, flow-through respirometry and O2 monitoring software included
This versatile new Oxygen Meter acts as both a stand alone oxygen meter, displaying and storing oxygen values, or as an interface to respirometry or monitoring software running on a PC. It accepts inputs from either one or two Strathkelvin 1302 microcathode oxygen electrodes, and shows the oxygen values on a large liquid crystal display. The display will show units of either PO2 (mm Hg; torr; KPa) or concentration (µmol/l; µg/l; mg/l; µl/ml; ml/l; ppm; or % saturation). When connected to the software (supplied, which is installed on a PC, it can be used for either closed cell respirometry (using the MT200 mitocell; RC300 or RC350 respiration cell) or for flow respirometry (using the FC100 flowcell or MC100 microcell). Alternatively the software can be used to monitor dissolved oxygen, as in isolated organ preparations. Details of the software are shown on the facing page.
The software used in the 782 meter and the 928 system is essentially similar. The main difference is that the 928 displays up to 6 traces, while the 782 has a maximum of two.
The program has been written so that it is extremely user-friendly and quick to learn. It can be used for closed cell respirometry (with the MT200, RC300, RC350 or RC650), or flow respirometry (using the FC100 or MC100) or for monitoring dissolved oxygen (using the FC 100 or MC100). When used for respirometry experiments, it follows the familiar logical sequences of operations, from electrode calibration to recording and analysis. The program follows a sequence of 3 main stages: Setting up, Recording, and Analysis.
The setup screen is used for entering all of the details which the computer needs in order to record in the correct units, and to express the respiration rate, normalized to biomass units if required, in the desired units. All experimental details entered here are transferred through to the final calculated results report page. During setup, the cell volumes (for closed cell) and flow rate, electrode positioning (for flow respirometry), temperature of the experiment, scrolling speed of the traces during recording, and the instruction to print the recording screen to printer (if hard copy is required) are all entered. Many of these will not change from one experiment to the next, and it is only necessary to enter changes.
Clicking the ‘Start’ button initiates the recording process, and traces then scroll across the screen. If substrates, inhibitors or other solutions require to be added during the run, the position where this occurs can be recorded with a flag, and the nature of the addition is recorded on a drop down menu. At the termination of the experiment, the data are saved to a data file, and may be analyzed straight away if required.
The data file, recorded traces, and event marker flags are recalled to the screen. If the recording has exceeded one screen width, it can be contracted to fit the screen, if required. Alternatively it is possible to scroll along long recording traces. Selector lines may be dragged to enclose specific parts of the trace. Thus if the traces show both control respiration and changed respiration after adding a solution, the selector lines would be dragged to the control rate first and then to the changed rates.
In each case respiration rates (normalized if so designated) are calculated automatically by clicking the ‘Calculate’ button. The calculated rates are tabulated, together with the experimental details entered during Setup, on the report page. The results may be exported to a spreadsheet, word processing or statistics package. The Analysis screen and the Report page may be printed, if required.
When the software is used for dissolved oxygen monitoring, the Analysis screen which displays the recorded traces and the oxygen values at flagged points or at set times can be transferred to the Results and report page.
The program requires a computer with a minimum specification of: Pentium processor, 1 free serial port, 64 Mb RAM, Windows 95 or later and 4 MB hard disk space. A comprehensive Instruction Manual is supplied, which will be particularly valuable to anyone who is not familiar with respirometric work.
This software is supplied with the 6-Channel Oxygen System Model 928 and the 1-2 Channel Oxygen Meter. It is not available separately.