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Home Electrophysiology Products Patch Clamp Equipment Accessories for PC-501A Patch Clamp Amplifiers


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Accessories for PC-501A Patch Clamp Amplifiers

The PC-501A Patch Clamp is a “workhorse” equally at home in research and teaching labs. Most single channel and whole cell studies are easily handled with this modestly priced instrument. With a choice of four headstages, a wide range of patch, whole cell and bilayer applications are covered. Its value is further enhanced by a 3-year warranty.
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Item Listing Full Description Specifications Journal Articles Manuals
Item# Description
640018 MC-10G Model Cell for 5101-10G (10 G?) Headstage
640019 MC-01G Model Cell for 5101-01G (1 G?) Headstage
640020 MC-100M Model Cell for 5101-100M (100 M?) Headstage
640021 MC-10GB Model Cell for 5101-10GB (10 G?) Bilayer Headstage

For local pricing, contact your nearest distributor.



Full Description Back to Top

• Independent V Hold and I Hold
• Stability in Current Clamp with selection of three I Clamp response speeds
• 4-Pole low-pass Bessel filter
• Internal generated test signals
• Zap circuit with variable duration
• 3 year warranty

The PC-501A Patch Clamp is a “workhorse” equally at home in research and teaching labs. Most single channel and whole cell studies are easily handled with this modestly priced instrument. With a choice of four headstages, a wide range of patch, whole cell and bilayer applications are covered. Its value is further enhanced by a 3-year warranty.

Headstage Choices
Four resistive-feedback headstages are available for the PC-501A:
• 5101-10G (10 G?) headstage for recording single channel currents up to ±1 nA. Noise level measured at 1 kHz is 60 fA.
• 5101-01G (1 G?) headstage for whole cell studies with currents to ±10 nA.
• 5101-100M (100 M?) headstage for whole cell studies with currents to ±100 nA.
• 5101-10GB (10 G?) headstage modified for bilayer capacitances up to 250 pF. Maximum current is ±1 nA.

Operating Modes
• V Clamp Mode: Input is clamped to a maximum of ±1 volt. Commands are the sum of the holding voltage and any external input signals.
• Zero Current Mode: Bridges the voltage and current clamp modes. All commands are inactive except the junction zero, which functions in this case as an offset control for the electrode and tip potentials associated with the pipette.
• I Clamp Mode: In current clamp mode, active commands are the current hold and any external command input signals, summed. Clamp stability is enhanced with the selection of three clamp speeds.

Compensation Controls
• Voltage Offsets (including junction potentials): are automatically compensated with the auto zero or manually with the junction zero.
• Capacity Compensation: Paired controls; fast (0-5 µsec), medium (0-2 msec) and slow (0-20 msec) compensate the current transient caused by a step command signal. Each pair has a separate control for amplitude and time constant.
• Series Resistance: Signal errors contributed by the electrode (access) resistance in whole cell recording are subtracted from the output with the series R comp control. The compensated resistance (0-100 M?) is read from a ten turn dial.
• Leak Subtract: Used to compensate leakage current due to the voltage drop across the seal (shunt) resistance.

Outputs/Signal Conditioning
The Im signal is bandwidth limited by a 4-pole Bessel filter with six frequency cut-offs and can be bypassed to obtain the full 10 kHz bandwidth. Gain is selected with a 7 position switch and scaled to the headstage resistor.

Additional outputs are Vm x 10 (membrane voltage at x10 gain) active in current clamp mode and ?Vc x 10, the sum of all commands at x10 gain. Rear panel outputs include; gain telegraph, a dc signal which communicates to a data acquisition system the Im output gain level and the headstage in use, and sync output, a signal to synchronize an oscilloscope or computer with one of the internal test signals.

Voltammetry with PC-501A/V
Simple modifications to the PC-501A make it suitable for voltammetric measurements. They include increasing the holding voltage potential to ±1 volt and the external voltage signal level to ±2 volts maximum. Internal switches can be installed to allow switching between normal [patch] and voltammetry operation. Contact our technical support department for details.


Specifications Back to Top

Specifications640018640019640020640021
CertificationsCECECECE

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Journal Articles Back to Top

Displaying 1 to 10 of 10results for Accessories for PC-501A Patch Clamp Amplifiers  

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Document

Description

100% Login to view WR15 PC501A Patch Clamp.pdf
[193 KB]
Patch Clamp Amplifier use in Activation of calcium entry in human carcinoma A431 cells
58% Login to view Activation of calcium entry in human carcinoma.pdf
[199 KB]
Patch Clamp Amplifier use in Activation of calcium entry in human carcinoma A431 cells
51% Login to view Plasma Membrane Calcium Channels in Human Carcinoma.pdf
[177 KB]
Patch Clamp Aplifier use in finding Plasma Membrane Calcium Channels in Human Carcinoma A431 Cells
40% Login to view WR18 PC501A .pdf
[150 KB]
Patch Clamp Aplifier use in finding Plasma Membrane Calcium Channels in Human Carcinoma A431 Cells
25% Login to view WR11 Oocyte Clamp Amplifier.pdf
[453 KB]
Oocyte Clamp Amplifier use in finding 4-Methylhomoibotenic Acid Activates a Novel Metabotropic Glutamate Receptor Coupled to Phosphoinostide
20% Login to view WR20 OC725C.pdf
[168 KB]
Inactivation of Kv2.1 Potassium Channels with use of Voltage-Clamp Amplifier
19% Login to view GABAB and Trk Receptor Signaling.pdf
[139 KB]
GABAb and Trk Receptor Signaling Mediates Long-Lasting Inhibitory Synaptic Depression
18% Login to view Inactivation of Kv2.1 Potassium Channels.pdf
[183 KB]
Inactivation of Kv2.1 Potassium Channels with use of Voltage-Clamp Amplifier
10% Login to view Effect of GABA on GnRH Neurons Switches from Depolarization.pdf
[262 KB]
Effect of GABA on GnRH Neurons Switches from Depolorization to Hyperpolarization at Puberty in the Female Mouse
10% Login to view OT10 captubes 2002.pdf
[262 KB]
Effect of GABA on GnRH Neurons Switches from Depolorization to Hyperpolarization at Puberty in the Female Mouse
   

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Manuals Back to Top

Displaying 1 to 1 of 1result for Accessories for PC-501A Patch Clamp Amplifiers  

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Document

Description

100% Login to view MicroIncubator PSMI.pdf
[121 KB]
PSMI Patch Slice Micro Incubator Manual
   

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