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Low Volume Vertical Ussing/Diffusion Chambers
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Low Volume Vertical Ussing/Diffusion Chambers
These chambers are designed in three formats for work with excised tissue segments. Tissue is affixed between the opposing faces of the half-chambers using a series of pins surrounding the opening.
Tissue diffusion chambers are constructed as two half-cells that are held together by a high tension spring retaining ring that insures leak free operation. They are available with both circular and oblong openings, depending on the tissue type under study. For example, for intestinal tissue studies, oblong openings can increase the effective surface area of the sample.
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• 14 Different chambers available in 3 configurations:
– Standard Chambers
– Low Volume Chambers
– Specialty Chambers
- Snapwell™
- Millicell®
These chambers are designed in three formats for work with excised tissue segments. Tissue is affixed between the opposing faces of the half-chambers using a series of pins surrounding the opening.
Tissue diffusion chambers are constructed as two half-cells that are held together by a high tension spring retaining ring that insures leak free operation. They are available with both circular and oblong openings, depending on the tissue type under study. For example, for intestinal tissue studies, oblong openings can increase the effective surface area of the sample.
Several low volume tissue chambers that reduce the amount of compound required to conduct permeability studies and custom chambers are also available. Up to six vertical chambers of any type can be placed in the heat block at one time, allowing convenient execution of six simultaneous transport studies under precise experimental conditions. The compact configuration, 38.1 x 10.2 x 12.7 cm (15 x 4 x 5 in), conserves valuable bench space. An optional heat block that can hold up to 24 chambers is also available. An optional adapter (perfusion cap) can be inserted into each half cell for closed perfusion applications.
Snapwell™ Diffusion Chambers
This chamber is specifically designed for use with the Snapwell™ culture insert (Corning Costar Corp.). The chamber accepts the lower section of the Snapwell, which contains the tissue culture treated membrane with cell monolayer. These Snapwell™ chambers give better results than other chambers. Their low profile reduces the unstirred water layer thus more closely approaching the real conditions in the intestine.
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