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Home Electrometer Intracellular Amplifier IE-210


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Electrometer Intracellular Amplifier IE-210

Model IE-210 is an intracellular microelectrode amplifier with low noise, low drift and fast response time for recording from high impedance fluid-filled glass electrodes. Current injection (bridge) circuitry permits simultaneous stimulation and recording through a single electrode. These features, in combination with a small lightweight headstage, a 4-pole Bessel low pass filter and optional probe breakaway make it an ideal instrument for easy, accurate and reliable intracellular recording and current injection.
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Item# Description
640039 IE-210 Intracellular Electrometer; Suppliedwith probe and rack mount hardware. Specify line operating voltage if other than 100 to 130 VAC.
640042 2100 Replacement Headstage for IE-210 Electrometer
640040 BB-15 Breakaway Box
640041 RB-1 Remote Buzz Control

For local pricing, contact your nearest distributor.



Full Description Back to Top

• High impedance microelectrode amplifier for intracellular studies
• Simultaneous stimulation and recording

Model IE-210 is an intracellular microelectrode amplifier with low noise, low drift and fast response time for recording from high impedance fluid-filled glass electrodes. Current injection (bridge) circuitry permits simultaneous stimulation and recording through a single electrode. These features, in combination with a small lightweight headstage, a 4-pole Bessel low pass filter and optional probe breakaway make it an ideal instrument for easy, accurate and reliable intracellular recording and current injection.

The Headstage
Fluid-filled microelectrodes can have resistances of over 100 megohms. To faithfully record potentials with such high resistances, the amplifier input resistance must be at least 100 times greater. The input resistance of the IE-210 is 5000 times greater (5 x 1011 ?) to assure accurate measurements with high resistance electrodes.
To maximize the bandwidth of voltage recording with high resistance electrodes, care must be taken to limit the input capacitance of the amplifier and minimize stray capacitance associated with the electrode and the cable which connects it to the amplifier. The IE-210 headstage is designed to minimize both. The headstage utilizes bootstrap circuitry and a driven shield to limit input capacitance to 0.5 pF. Stray input capacitance is reduced with two design features of the headstage: 1) the pencil-sized headstage mounts directly on the micromanipulator, therefore the electrode holder can connect directly to the headstage input and requires no connecting cable; 2) the headstage driven shield is available at the probe input to permit attachment of additional shielding. This allows the driven shield to be extended to surround the electrode holder and further reduce the stray input capacitance. This method of capacitance neutralization is preferable to the common method of capacitance neutralization since capacitance compensation circuitry can increase noise levels.

Electrometer Features
The probe/electrometer output is available at a gain of one. Amplifier gain is increased to x10 at the current injection Bridge Output. Input junction potentials can be compensated with the Input Offset control. If desired, remaining stray input capacitance, up to 50 pF, can be neutralized with Cap Comp (capacity compensation). Convenient electrode resistance measurement is available using Electrode Test which produces a voltage proportional to the electrode resistance. Cell impalement is facilitated with a Buzz voltage (variable in both frequency and amplitude) applied to the electrode. An overload condition at the input (blocked electrode or oscillations caused by excessive capacity compensation) is indicated by the Input Overload light.

Current Injection
Simultaneous stimulation and recording with a single electrode is made possible with an active bridge circuit. Stimulus currents are generated either internally with the DC Current source or by a voltage applied to the external Stimulus Input. The internal source supplies currents up to 100 nA in the Low range and up to 1000 nA in the High range. The current injection feature is often used for iontophoretic injection of dyes or drugs. External signals can be of any waveform or DC level. Internal and external stimulus currents are summed when applied simultaneously.

Voltage drops across the electrode are subtracted from the Bridge Output using the DC Balance and AC Balance controls. The remaining output represents the cell’s response to the applied current. Electrode resistance can be read directly from the DC Balance dial. Gain of the Bridge Output signal is x10 at the front panel and can be switch-selected to x20 or x50 at the rear panel. The output signal is available at either the full amplifier bandwidth (30 kHz) or filtered with the internal 4-pole Bessel low pass filter.

Additional Features
• Meter: The 3-1/2 digit LED meter provides readouts of the x1 Amplifier Output voltage and the stimulus DC Current, and is easily viewed in a darkened room.
• Probe Test: Checks of amplifier operation are quick and convenient with the front panel Probe Test facility, a real time-saver when troubleshooting set-up problems.
• Differential Amplifier: Signals from another amplifier can be applied to the Differential Input and are then summed with the IE-210 x1 output.
• Filter Telegraph: The filter frequency is encoded by a stepped DC voltage for input to a data acquisition system.

Electrode Holder
Electrode holder is not included with the IE-210. Please purchase holder separately based on your glass outer diameter. Electrical coupling between the electrode and headstage can be either a Ag wire or Ag-AgCl pellet and is easily replaceable. The holder is supplied with the silver wire installed and a pellet is included for conversion to a pellet type holder.

Optional Accessories
Remote Buzz (RB-1) control provides convenient remote operation of the Buzz voltage. It is supplied with a 3 m length cable.

Breakaway Box (BB-15) permits the application of large voltages to the electrode for iontophoretic injection of dyes or drugs. Voltages up to 200 V may be applied to the Breakaway input without damage to the probe input. Breakaway Current = applied voltage (electrode resistance + 2 M?).


Specifications Back to Top

Specifications640039640042640040640041
Amplifier: Input Impedance5 x 1011 ? shunted By 0.5 pF   
Amplifier: Noise Level(0.1 Hz to 10 kHz)*
25 V p-p input shorted
250 V p-p, 20 M? at input
* At x1 output.
   
Amplifier: Output Resistance100 ?*na ?*na ?*na ?
Amplifier: Gainsx1 0.1%   
Amplifier: Rise Time (10 to 90%)25 sec, measured through 20 M?   
Amplifier: Capacity Compensation0 to 50 pF   
Amplifier: Probe Input Voltage Range10 V   
Amplifier: Leakage Current1 pA typical, adjustable to zero   
Amplifier: Electrode Test1 mV/M? (Low Range)
10 mV/M? (High Range)
   
Amplifier: Input Offset200 mV, referred to input   
Buzz: Amplitude Variable0-15 V   
Buzz: Frequency Variable100 Hz to 10 kHz   
Current Injection: Bridge Balance Ranges0 to 100 M? & 0 to 1000 M? (Low Range)
0 to 10 M? & 0 to 100 M? (High Range)
   
Current Injection: Stimulus Input Resistance10 k?*na k?*na k?*na k?
Current Injection: Maximum Stimulus Input15 V   
Current Injection: Bridge Bal. Output Resistance100 ?*na ?*na ?*na ?
Current Injection: I Monitor Output100 mV/nA (Low Range)
10 mV/nA (High Range)
   
Current Injection: Internal DC Current Source0 to 100 nA (Low Range)
0 to 1000 nA (High Range)
   
Current Injection: External StimulusCurrent is limited by electrode resistance up to maximums shown:
10 V electrode R or 0.5 A (Low Range)
10 V electrode R or 5 A (High Range)
   
Current Injection: Bridge Output Gainx10 at front panel
x20 or x50 at rear panel
   
Current Injection: Bridge Output Filter4-pole low pass Bessel with cut-offs at 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10 and 20 kHz   
Power Requirements100 to 130 or 220 to 240 VAC, 50/60 Hz, 10 VA   
Case Height Metric8.9 cm*na cm*na cm*na cm
Case Width Metric43.2 cm*na cm*na cm*na cm
Case Depth Metric30.5 cm*na cm*na cm*na cm
Probe Diameter Metric12.5 mm*na mm*na mm*na mm
Probe Length Metric6.2 cm*na cm*na cm*na cm
Shipping Weight Metric5.5 kg*na kg*na kg*na kg
WarrantyThree years, parts & labor   
Meter3-1/2 digit LED, Full Scale Ranges: Amplifier Output: 200 mV, 2 V and 20 V Current: 200 nA and 2 A   
CertificationsCECECECE

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Journal Articles Back to Top

Displaying 1 to 10 of 28results for Electrometer Intracellular Amplifier IE-210   Page 1  2  3 

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Document

Description

100% Login to view Intracellular Calcium Oscillations Signal Apoptosis.pdf
[826 KB]
PicoInjector use in Intracellular Calcium Oscillations Signal Apoptosis
70% Login to view HB07 bsc 1.pdf
[582 KB]
Intracellular Response Properties of Units in the Dorsal Cochlear Nucleus of Unanesthetized Decerebrate Gerbil
55% Login to view WR20 OC725C.pdf
[168 KB]
Inactivation of Kv2.1 Potassium Channels with use of Voltage-Clamp Amplifier
52% Login to view HS14 vent and amp.pdf
[431 KB]
Ventilator and Amplifier use in Hyperventilation Inducing release of Cytokines from Perfused Mouse Lung
50% Login to view Inactivation of Kv2.1 Potassium Channels.pdf
[183 KB]
Inactivation of Kv2.1 Potassium Channels with use of Voltage-Clamp Amplifier
45% Login to view A Protein Kinase C Site Highly Conserved in P2X Subunits.pdf
[331 KB]
OC-725B amplifier use in finding A Protein Kinase C Site
45% Login to view WR21 OC725 Amplifier.pdf
[324 KB]
OC-725B amplifier use in finding A Protein Kinase C Site
42% Login to view Activation of calcium entry in human carcinoma.pdf
[199 KB]
Patch Clamp Amplifier use in Activation of calcium entry in human carcinoma A431 cells
42% Login to view WR15 PC501A Patch Clamp.pdf
[193 KB]
Patch Clamp Amplifier use in Activation of calcium entry in human carcinoma A431 cells
42% Login to view HS14 Hyperventilation and Cytokine Release.pdf
[422 KB]
Ventilator and Amplifier use in Hyperventilation Inducing release of Cytokines from Perfused Mouse Lung
   

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Manuals Back to Top

Displaying 1 to 1 of 1result for Electrometer Intracellular Amplifier IE-210  

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Description

100% Login to view 600123 temp amp.pdf
[75 KB]
Temperature amplifier manual
   

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