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Home PCLS - Isolated Perfused Precision Cut Lung Slice System


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PCLS - Isolated Perfused Precision Cut Lung Slice System

Lung Functions under the Microscope
Precision-cut lung slices (PCLS) offer a novel and unique way to assess lung functions under cell culture conditions. They can be prepared from nearly any species including murine, rat and human lungs. The method allows the study of the response of airways of different size (down to the terminal bronchioles) and to relate these changes in lung functions to gene expression and mediator release. Slices are viable for at least three days. They can be placed under an inverted microscope, where digital image techniques allow to quantify not only the responsiveness of single airways, but also of single vessels. In addition, it is possible to analyze the ciliary beating frequency. More than 20 slices can be obtained from one lung, thus this method is very economical in terms of experimental costs and animal use. Tissue cores are prepared from the lungs filled with agarose solution, after cooling to 4C. From the cores, slices (220 20m) are cut using a Krumdieck tissue slicer.

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739921 PCLS - Isolated Perfused Precision Cut Lung Slice System

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• Assessment of lung functions under cell culture conditions
• From any species (murine, rat, human)
• Study of airways of different sizes
• Allows to quantify responsiveness of simple airways and simple vessels
• Analyzing ciliary beating frequency

Lung Functions under the Microscope
Precision-cut lung slices (PCLS) offer a novel and unique way to assess lung functions under cell culture conditions. They can be prepared from nearly any species including murine, rat and human lungs. The method allows the study of the response of airways of different size (down to the terminal bronchioles) and to relate these changes in lung functions to gene expression and mediator release. Slices are viable for at least three days. They can be placed under an inverted microscope, where digital image techniques allow to quantify not only the responsiveness of single airways, but also of single vessels. In addition, it is possible to analyze the ciliary beating frequency. More than 20 slices can be obtained from one lung, thus this method is very economical in terms of experimental costs and animal use. Tissue cores are prepared from the lungs filled with agarose solution, after cooling to 4C. From the cores, slices (220 20m) are cut using a Krumdieck tissue slicer
The incubation chamber was developed to allow incubation and observation of slices by an inverted microscope. The chamber is made of Polycarbonate. It is connected to a water bath to maintain constant temperature conditions. Two incubation cells are positioned in the center of the chamber. The bottom of the cells is sealed by glass, the cover is made of acrylic glass. The slices are fixed in the incubation cells by positioning them under nylon strings fixed to a bent platinum wire. The incubation cells can be filled with buffer, medium or drug solutions through the filling pipe. Buffer solution can be removed from the cells over a vacuum pipe. In addition, it is possible to gas the incubation cells in order to use bicarbonate buffered media.
The incubation chamber is placed on the stage of an inverted microscope and warmed to 37C. The slices are screened for airways and transferred to the incubation chamber. Lung slices are selected for study using predefined criteria (Martin et al. 1996). Airways and vessels are focused, and finally the images are analyzed by image analysis software (e.g., Optimas or Metamorph).
Example of an Application
As an example Figure 1 shows exposure of a slice to increasing concentrations of endothelin-1. Shown is a lung slice containing an airway (B), a pulmonary artery (PA) and a pulmonary vein (PV). The pulmonary artery and the airway contracted almost completely, while the pulmonary vein area decreased to only 50% of its initial area. These responses are now easily quantified by digital imaging technique.
It is a distinct advantage of this technique that in many ways precision-cut slices can be treated like a cell culture. Thus, the slices can be incubated under various conditions and gene as well as protein expression or mediator release be determined. In contrast to cell culture models, in slices the anatomical structure of the lung is largely maintained, so that the functional consequences of gene expression and mediator release can be evaluated.


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Displaying 1 to 10 of 43results for PCLS - Isolated Perfused Precision Cut Lung Slice System   Page 1  2  3  4  5 

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100% Login to view HS14 Hyperventilation and Cytokine Release.pdf
[422 KB]
Ventilator and Amplifier use in Hyperventilation Inducing release of Cytokines from Perfused Mouse Lung
94% Login to view HS14 vent and amp.pdf
[431 KB]
Ventilator and Amplifier use in Hyperventilation Inducing release of Cytokines from Perfused Mouse Lung
92% Login to view VT01 2,3-Butanedione Monoxime Cardioplegia Advantages Over Hyperkalemia in Blood-Perfused Isolated Hearts.pdf
[199 KB]
2,3-Butanedione Monoxime Cardioplegia: Advantages Over Hyperkalemia in Blood-Perfused Isolated Hearts
86% Login to view HS15 isolated heart app.pdf
[632 KB]
Isolated Heart Apparatus use in Myoglobin facilitating oxygen diffusion
64% Login to view PP45 Syringe Pump.pdf
[764 KB]
Precision Alignment Packaging For Microsystems with Multiple Fluid Connections
54% Login to view PP93.pdf
[1.4 MB]
Gene transfer of dominant-negative RhoA increases outflow facility in perfused human anterior segment cultures
44% Login to view OT04.pdf
[609 KB]
A new planar multielectrode array for extracellular recprding: application to hippocampal acute slice
42% Login to view PP45 Precision Alignment Packaging for Microsystems with Multiple Fluid Connections.pdf
[301 KB]
Union of capillary high-performance liquid chromatography
42% Login to view VT27 683 Ventilator.pdf
[503 KB]
Critical role for CXCR2 and CXCR2 ligands during pathogenesis of ventilator-induced lung injury
35% Login to view HS15 Myoglobin facilitates oxygen diffusion.pdf
[631 KB]
Isolated Heart Apparatus use in Myoglobin facilitating oxygen diffusion
   

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Displaying 1 to 3 of 3results for PCLS - Isolated Perfused Precision Cut Lung Slice System  

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100% Login to view Isolated Preamplifier.pdf
[126 KB]
Isolated Preamplifier manual
78% Login to view 650073 76 Brain Slice Chmbr Manual.pdf
[642 KB]
650073-0076_Manual.qxd
60% Login to view VT0168 1.PDF
[229 KB]
2,3-Butanedione Monoxime Cardioplegia: Advantages Over Hyperkalemia in Blood-Perfused Isolated Hearts
   

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