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Home Pico-Injector Microinjection Systems


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Pico-Injector Microinjection Systems

The PLI-100A Pico-Injector reliably delivers injections from femtoliters to nanoliters through micropipettes by applying a regulated pressure for a digitally set period of time.

  • Femtoliter to microliter injections
  • Digital readouts for injection pressure, time, and count
  • Reliable optically encoded circuit for injection time set
  • 5 pressures: inject, balance, clear, fill and hold.

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Item Listing Full Description Specifications Journal Articles Manuals
Item# Description
641735 Model PLI-100A Basic Picoinjector with injection, balance, clear, filling and holding pressures. Comes with input hose, output hose, holding hose, and input hose adapter.
641736 Model PLI-100A Plus Picoinjector with injection, balance, clear, filling and holding pressures. Comes with input hose, output hose, holding hose, foot switch, pipette holder, and input hose adapter.
641737 Model PLI-100A Deluxe Picoinjector with injection, balance, clear, filling and holding pressures, comes with input hose, output hose, holding hose, two foot switches, two pipette holders, and input hose adapter.

For local pricing, contact your nearest distributor.



Full Description Back to Top

Pressure to the pipette is controlled precisely through a multi-turn regulator, and is reported digitally for reproducibility. Injection time is set using an optically encoded circuit which permits fine and coarse settings from a single knob.

The system timer can be controlled in three ways, front panel push button, foot switch, and external trigger input BNC. Whether you need to do large injections into capillaries or very small injections into mammalian nuclei, the PLI-100A is up for the task.

Pressure Capabilities
The PLI-100A features two negative and three positive pressure pneumatic capabilities. The negative or vacuum functions allow the user to:

1. Fill micropipettes from their tips, reducing waste of pipettes.
2. Provide a means to secure and manipulate a cell using holding pipette. The positive pressures allow the user to:
  • Eject precise amounts of fluids
  • Create a balance pressure which prevents backflow into the micropipette following an injection
  • Clear a micropipette of material in it

Unique Features
The PLI-100A's important 'Balance', 'Hold' and 'Clear' functions are features not found on other microinjection units.

Balance
In addition to the ejection pressure, the Pico-Injector offers a secondary balance pressure. This adjustable pressure keeps a positive pressure on the injection pipette before and after injections. This eliminates dilution caused by capillary action and aids in the prevention of clogging. Wasteful continuous injection, which often occurs when no separate balance pressure is offered by an injection device, is avoided.

Fill/Hold
There are two built-in vacuum generators to fill a micropipette from the tip and to hold suspended cells. Filling the pipette from the tip is easier than back filling. The PLI-100A features a Fill Vacuum adjustment on the front panel. Suspended cells can be held with a second (holding) pipette. The holding vacuum's range accommodates most cell types.

Clear
A high-pressure pulse can be used for clearing a pipette, should it happen to clog. This is particularly useful when working with pipettes sized for smaller volumes.

Electrical Connectors
BNC type connectors are available at the front panel to ease integrating the Pico-Injector with other equipment. Synchronization of injections to other stimulations or recordings is therefore possible.

Selection Guideline
The PLI-100A is available in three packages: Basic, Plus, and Deluxe. The Basic unit is supplied with an input hose, output hose, holding hose, and input hose adapter. The Plus unit also includes a foot switch, pipette holder, and input hose adapter. The Deluxe unit includes all the accessories included with the Plus and an additional foot switch and pipette holder.


Specifications Back to Top

Specifications641735641736641737
ModelPLI-100APLI-100A PlusPLI-100A Deluxe
Input Gas Pressure75 PSI Recommended, 105 PSI Maximum75 PSI Recommended, 105 PSI Maximum75 PSI Recommended, 105 PSI Maximum
Clearing PressureInput gas pressure, unregulatedInput gas pressure, unregulatedInput gas pressure, unregulated
Injection Pressure0.2 to 60 PSI (413 KPa), regulated0.2 to 60 PSI (413 KPa), regulated0.2 to 60 PSI (413 KPa), regulated
Balance Pressure0.1 to 9.9 PSI (68.9 KPa) regulated, multiturn control, other ranges available on request0.1 to 9.9 PSI (68.9 KPa) regulated, multiturn control, other ranges available on request0.1 to 9.9 PSI (68.9 KPa) regulated, multiturn control, other ranges available on request
Fill VacuumInternally produced 0 to -12 PSI (0 to-82 KPa), regulatedInternally produced 0 to -12 PSI (0 to-82 KPa), regulatedInternally produced 0 to -12 PSI (0 to-82 KPa), regulated
Holding VacuumInternally produced 0 to -0.1 PSI (0 to -0.75 KPa), regulatedInternally produced 0 to -0.1 PSI (0 to -0.75 KPa), regulatedInternally produced 0 to -0.1 PSI (0 to -0.75 KPa), regulated
Power InputExternal 9VDC @ 400mA (min) Power Supply universal input 90 to 264 VACExternal 9VDC @ 400mA (min) Power Supply universal input 90 to 264 VACExternal 9VDC @ 400mA (min) Power Supply universal input 90 to 264 VAC
Injection Time0.01 to 99.99 Seconds0.01 to 99.99 Seconds0.01 to 99.99 Seconds
Injection Count Display0 to 9999 Injections0 to 9999 Injections0 to 9999 Injections
Duration ModeInternally timed or externally gatedInternally timed or externally gatedInternally timed or externally gated
Time TriggerFront panel, foot switch, or external TTL pulse (BNC)Front panel, foot switch, or external TTL pulse (BNC)Front panel, foot switch, or external TTL pulse (BNC)
Pressure UnitsPSI /KPa switch selectablePSI /KPa switch selectablePSI /KPa switch selectable
Pressure Monitor OutputBNC, Rear Panel, 10 mV/PSI or 1 mV/KPaBNC, Rear Panel, 10 mV/PSI or 1 mV/KPaBNC, Rear Panel, 10 mV/PSI or 1 mV/KPa
Pressure ReadoutInject, balance, clear, output portInject, balance, clear, output portInject, balance, clear, output port
Weight5.44 kg (12 lb)5.44 kg (12 lb)5.44 kg (12 lb)
Injection Time Accuracy±0.01% (Crystal Time Base)±0.01% (Crystal Time Base)±0.01% (Crystal Time Base)
Pressure Display Resolution3˝ Digits, 0.1 PSI or 1 KPa3˝ Digits, 0.1 PSI or 1 KPa3˝ Digits, 0.1 PSI or 1 KPa
Dimensions (HxWxD)89 x 432 x 250 mm (H x W x D)89 x 432 x 250 mm (H x W x D)89 x 432 x 250 mm (H x W x D)
WarrantyOne year, parts & laborOne year, parts & laborOne year, parts & labor

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Journal Articles Back to Top

Displaying 1 to 10 of 24results for Pico-Injector Microinjection Systems   Page 1  2  3 

Score

Document

Description

100% Login to view PI02.pdf
[431 KB]
Pico-Injector use in Inhibition of the Inositol Trisphosphate Receptor of Mouse Eggs and A7r5 Cells
83% Login to view Inhibition of the Inositol Trisphosphate Receptor of Mouse Eggs.pdf
[440 KB]
Pico-Injector use in Inhibition of the Inositol Trisphosphate Receptor of Mouse Eggs and A7r5 Cells by KN-93 via a Mechanism Unrelated to Ca2 /Calmodulin-dependent Protein Kinase II Antagonism
78% Login to view Zebrafish mitotic kinesin-like protein 1 functions in embryonic cytokinesis.pdf
[1.1 MB]
Zebrafish mitotic kinesin-like protein 1 (Mklp1) functions in embryonic cytokinesis
58% Login to view BOR Papers in Press. Published on December 3, 2003.pdf
[289 KB]
Patterns of Intracellular Calcium Oscillations in Horse Oocytes Fertilized by Intracytoplasmic Sperm Injection
51% Login to view The Red Tide Toxin, Brevetoxin, Induces Embryo Toxicity and Developmental Abnormalities.pdf
[124 KB]
PL-100, The Red Tide Toxin, Brevetoxin, Induces Embryo Toxicity and Developmental Abnormalities
51% Login to view Intracellular Calcium Oscillations Signal Apoptosis.pdf
[826 KB]
PicoInjector use in Intracellular Calcium Oscillations Signal Apoptosis
51% Login to view PI15 plihorse.pdf
[278 KB]
Patterns of Intracellular Calcium Oscillations in Horse Oocytes Fertilized by Intracytoplasmic Sperm Injection
41% Login to view Ca21-Independent Protein Kinase C Apl II Mediates.pdf
[372 KB]
Ca2+ Independent Protein Kinase C Apl II Mediates the Serotonin-Induced Facilitation at Depressed Aplysia Sensorimotor Synapses
41% Login to view PI10 PLI 100.pdf
[364 KB]
Ca2+ Independent Protein Kinase C Apl II Mediates the Serotonin-Induced Facilitation at Depressed Aplysia Sensorimotor Synapses
41% Login to view Isoforms of the Inositol 1,4,5-Trisphosphate Receptor Are Expressed in Bovine Oocytes and Ovaries.pdf
[309 KB]
Isoforms of the Inositol 1,4,5-Triphosphate Receptor Expressed in Bovine Oocytes and Ovaries
   

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Manuals Back to Top

Displaying 1 to 2 of 2results for Pico-Injector Microinjection Systems  

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Document

Description

100% Login to view PicoInjector PLI90.pdf
[110 KB]
Model PLI-90 Pico Injector Manual
89% Login to view PicoInjector PLI100.pdf
[949 KB]
Model PLI-100 Pico Injector Manual
   

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